Gba (NM_001077411) Mouse Tagged ORF Clone

CAT#: MR230333

  • TrueORF®

Gba (myc-DDK-tagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 2

AAV Particle: DDK


  "NM_001077411" in other vectors (1)


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Reconstitution Protocol

USD 479.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Gba
Synonyms betaGC; GBA1; GC; GCase; GLUC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR230333 representing NM_001077411
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGCA
TCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGACCC
CGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAGCTG
AGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAGAAGT
TCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTGTCCCC
ACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATCCGGGTA
CCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTCCAGTTAT
CCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTGAAGATGTC
CTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAAGAGTG
AATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAATTACTTTGTCA
AGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAATGAACCTACGGC
AGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGAGACTTCATTTCC
CGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTAGATGACCAACGCT
TGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTACGTTCATGGCATTGC
TGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
AACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAGAGTGTTCGGTTGGGCT
CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
GGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTTGTCGATAGCCCCATT
ATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
TCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACTGACTTGGAAACAGTAGCACT
GTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCGGAGGATGTCCCTCTTACCATC
AGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCCATTCACACTTACCTGTGGCGTC
GCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR230333 representing NM_001077411
Red=Cloning site Green=Tags(s)

MAARLIGFFLFQAVSWAYGAQPCIPKSFGYSSVVCVCNASYCDSLDPVTLPALGTFSRYESTRRGRRMEL
SVGAIQANRTGTGLLLTLQPEKKFQKVKGFGGAMTDATALNILALSPPTQKLLLRSYFSTNGIEYNIIRV
PMASCDFSIRVYTYADTPNDFQLSNFSLPEEDTKLKIPLIHQALKMSSRPISLFASPWTSPTWLKTNGRV
NGKGSLKGQPGDIFHQTWANYFVKFLDAYAKYGLRFWAVTAENEPTAGLFTGYPFQCLGFTPEHQRDFIS
RDLGPALANSSHDVKLLMLDDQRLLLPRWAEVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFP
NTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPI
IVDIPKDAFYKQPMFYHLGHFSKFIPEGSQRVALVASESTDLETVALLRPDGSAVVVVLNRSSEDVPLTI
SDPDLGFLETVSPGYSIHTYLWRRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001077411
ORF Size 1545 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001077411.1, NM_001077411.2, NP_001070879.1
RefSeq Size 2069 bp
RefSeq ORF 1548 bp
Locus ID 14466
UniProt ID P17439
Cytogenetics 3 39.01 cM
MW 57.6 kDa
Gene Summary Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity). Also plays a role in cholesterol metabolism (PubMed:24211208). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity).[UniProtKB/Swiss-Prot Function]

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