Camk2d (NM_001293663) Mouse Tagged ORF Clone

CAT#: MR230321

Camk2d (myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, delta (Camk2d), transcript variant 4

Product Images

Circular map for MR230321

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camk2d
• Synonyms: 2810011D23Rik; 8030469K03Rik; CaMK II; [d]-CaMKII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230321 representing NM_001293663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGACCACCACCTGCACCCGGTTCACCGACGAGTATCAGCTCTTTGAGGAGCTCGGAAAGGGGG
  CGTTCTCAGTGGTGAGAAGATGTATGAAAATCCCTACTGGACAAGAGTATGCTGCCAAAATTATCAACAC
  CAAAAAGCTTTCTGCTAGGGACCATCAGAAACTGGAAAGGGAAGCTAGAATCTGCCGTCTCTTGAAGCAC
  CCCAATATTGTGAGACTTCACGACAGTATATCGGAGGAGGGCTTCCATTACTTGGTGTTTGACTTAGTGA
  CTGGTGGCGAACTGTTTGAAGACATAGTGGCAAGAGAATATTACAGTGAAGCTGATGCCAGTCATTGTAT
  ACAACAGATTCTAGAGAGTGTAAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAG
  AATTTGCTTTTAGCTAGCAAGTCCAAAGGAGCAGCTGTGAAGCTGGCAGACTTCGGCTTAGCCATAGAAG
  TTCAAGGCGACCAGCAGGCATGGTTTGGTTTTGCTGGCACACCTGGGTATCTTTCTCCAGAAGTCCTGCG
  TAAAGATCCTTATGGAAAACCAGTGGATATGTGGGCATGCGGTGTCATCCTCTACATCTTGCTGGTGGGA
  TACCCACCCTTCTGGGATGAAGATCAGCATAGACTGTATCAGCAGATCAAGGCCGGAGCTTACGATTTTC
  CGTCACCAGAATGGGATACAGTGACACCTGAAGCCAAAGACCTCATCAACAAAATGCTGACCATCAACCC
  TGCCAAACGTATCACAGCCTCTGAGGCCCTGAAACACCCATGGATCTGTCAACGCTCTACTGTTGCCTCC
  ATGATGCACAGGCAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGACGGAAACTGAAGGGCGCCA
  TCTTGACAACTATGCTGGCTACGAGAAATTTTTCAGCAGCCAAGAGTTTATTGAAGAAACCAGATGGGGT
  AAAGATAAACAACAAAGCCAACGTGGTAACCAGCCCCAAAGAAAATATTCCTACCCCGGCGCTGGAGCCC
  CAAACTACTGTAATCCACAACCCTGACGGAAACAAGGAGTCAACTGAGAGCTCAAACACCACCATTGAGG
  ATGAAGACGTGAAAGCACGAAAACAGGAGATCATCAAAGTCACTGAGCAACTGATTGAAGCTATCAACAA
  TGGGGACTTTGAGGCTTACACAAAAATCTGTGATCCAGGCCTCACTGCCTTTGAACCTGAAGCATTGGGC
  AACTTAGTGGAAGGGATGGACTTTCACAGATTCTACTTTGAAAATGCTTTGTCCAAAAGCAATAAACCAA
  TCCACACGATCATCCTCAACCCACACGTTCACCTGGTAGGGGATGACGCAGCCTGCATCGCATACATTCG
  GCTCACACAGTACATGGACGGAAGCGGGATGCCAAAGACCATGCAGTCAGAAGAGACGCGCGTGTGGCAC
  CGCCGTGATGGGAAGTGGCAGAATGTTCACTTTCACCGTTCGGGGTCCCCCACAGTACCCATCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230321 representing NM_001293663
  Red=Cloning site Green=Tags(s)
  
  MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKH
  PNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPE
  NLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVG
  YPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVAS
  MMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKINNKANVVTSPKENIPTPALEP
  QTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALG
  NLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWH
  RRDGKWQNVHFHRSGSPTVPIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293663
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293663.1, NP_001280592.1
• RefSeq Size: 5615 bp
• RefSeq ORF: 1539 bp
• Locus ID: 108058
• UniProt ID: Q6PHZ2
• Cytogenetics: 3 G1
• MW: 58.2 kDa
• Gene Summary: Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.[UniProtKB/Swiss-Prot Function]