Fbxw27 (NM_001303003) Mouse Tagged ORF Clone

CAT#: MR230053

Fbxw27 (myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 27 (Fbxw27), transcript variant 1

Product Images

Circular map for MR230053

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxw27
• Synonyms: 1700124P09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230053 representing NM_001303003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCATTTGCCTAGTTTGCCGTTGATGAAAATCTTCTCCTATCTGGATGCCTACAGTTTGCTAC
  AGGTTGCCCAAGTGAACAAGAACTGGAATGCACTTGCAAGCAGTGATGTCCTGTGGAGGAAGTTGTGTCA
  GAAGAGATGGCTTTTCTGTGACATGGTCACCCTACAGCTCCAGGACAAAGAGACATGGAAGCAGTTCTTC
  GTTTACCGAACGTGGCAAGAACACTCCAAGACCCGGGCAAATCCACGAGATTTCACTTACAAGGAAATTC
  CTTTGCAATATGGACCTTGTGGCTATGCATGTTATATCTCAGGGTGTGGCATAACAAGAAAGGGACAAGA
  CAAGTCTGTTGTCTGTATGGTGAATTCCAAGAACACGATTTCCACCTGGGATGTTCACAAGAGCGTTATA
  ACCTGGGTAAGCCCAGAACAGCCGGCAAGTATCAAGCTGTTGACTACCCTCCCTGAGATGCACATTGCAG
  TCACTGTAGATATTCAATCAACTATCAAACTGTGGGACTGTCACAACAGGGAAGCTCTGGCGACTAACAT
  CCTGGAATCTCCATGCAAATCACTGAAAGCTGTCATTACCAAGGATGACCCGATTGTCTTGGCAGGCGAT
  ATCTTGGGTAACCTGTACATCTTTAGAATTCCAGATTTACACCTCATTTCCACAGTCAATGTATTGCCAT
  GTGATATTGATGAAATCTACTGCTCTCCTCAGAAGAAATGGGTCTTACTGAGTAAGAAACAGCCATATAT
  TTTGCCAAAGGTGTTTTACATGAGCAGCCTACTGAGGACATCAGAATTCTCTGCCCCTGTGTCTACCCTT
  CTGGAAATTTCCTTATGTCAAGAAGCCTTCTGGACCCCAAGAAGGGAAGACAGGCTAACCCTGATGTCCA
  GAAGTGGTTGCTCCAAAATCACAAAGTTTGAAACATATGATATGAAGCTGGAAGAGTTTGGGAACAAAAT
  TATTGTTAAAGGAAATTCGATAGCAGGCTTTTTGTTGCCATATTACACGGAAAGCCTGAACTGGTTTGGA
  GTCAGTGATAAGGACACGATTGTTTGTTCAACTGAATTCTCTCTCTTGCTCTTTACCAATAATGGTCACC
  GTCTGCAGACCTTTCAGTACTTCTCAGAAACGATCGTGAGACTCTCTGTGGACCCCGTTCATGTCATCGT
  CACCTGTAATGATGGCTCTTTGGATGTATATGCATGGGAGGAGAGAAGCCTGCTGCTCAGGAAGTGTTAT
  CGACTGAAAAACAGGAGACATCTGTCACCGAGAGGCTTCATTAACAAAACGCTATGTGATGATGTGAGCA
  TAATTCAGTTGATGATAGATGGTCATGACACCTGGTTTCTGATGGCATATGCCTTGAATATCTGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230053 representing NM_001303003
  Red=Cloning site Green=Tags(s)
  
  METHLPSLPLMKIFSYLDAYSLLQVAQVNKNWNALASSDVLWRKLCQKRWLFCDMVTLQLQDKETWKQFF
  VYRTWQEHSKTRANPRDFTYKEIPLQYGPCGYACYISGCGITRKGQDKSVVCMVNSKNTISTWDVHKSVI
  TWVSPEQPASIKLLTTLPEMHIAVTVDIQSTIKLWDCHNREALATNILESPCKSLKAVITKDDPIVLAGD
  ILGNLYIFRIPDLHLISTVNVLPCDIDEIYCSPQKKWVLLSKKQPYILPKVFYMSSLLRTSEFSAPVSTL
  LEISLCQEAFWTPRREDRLTLMSRSGCSKITKFETYDMKLEEFGNKIIVKGNSIAGFLLPYYTESLNWFG
  VSDKDTIVCSTEFSLLLFTNNGHRLQTFQYFSETIVRLSVDPVHVIVTCNDGSLDVYAWEERSLLLRKCY
  RLKNRRHLSPRGFINKTLCDDVSIIQLMIDGHDTWFLMAYALNICS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303003
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303003.1, NP_001289932.1
• RefSeq Size: 1497 bp
• RefSeq ORF: 1401 bp
• Locus ID: 76998
• Cytogenetics: 9 F2
• MW: 54.1 kDa