Lox (NM_001286182) Mouse Tagged ORF Clone

CAT#: MR229764

Lox (myc-DDK-tagged) - Mouse lysyl oxidase (Lox), transcript variant 3

Product Images

Circular map for MR229764

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lox
• Synonyms: AI893619; rrg; TSC-16; TSC-160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229764 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTTCGCCTGGGCTGTGCTCCTTCTGGGGCCACTGCAGCTTTGTCCCCTTCTCCGCTGCGCCCCGC
  AGACCCCGCGCGAGCCGCCCGCCGCCCCTGGTGCCTGGCGCCAGACAATCCAATGGGAGAACAACGGGCA
  GGTGTTCAGTCTGCTGAGCCTCGGGGCGCAGTACCAGCCTCAGCGACGCCGCGACCCCAGTGCCACTGCC
  CGGAGACCCGACGGCGACGCAGCCTCGCAGCCGCGCACGCCCATTCTTCTGCTGCGTGACAACCGCACCG
  CCTCTACCCGTGCGAGGACGCCAAGCCCGTCTGGGGTCGCCGCGGGTCGTCCCCGGCCCGCCGCCCGCCA
  CTGGTTCCAAGCTGGTTTCTCGCCGTCGGGGGCTCGCGATGGAGCCTCACGGCGCGCGGCGAACCGGACT
  GCATCGCCACAGCCTCCGCAGCTCAGTAATCTGAGGCCACCCAGCCACATAGATCGCATGGTGGGCGACG
  ACCCCTACAATCCCTACAAGTACTCCGACGACAACCCCTATTATAACTACTATGACACGTATGAGAGGCC
  CCGGCCCGGGAGCAGGAACCGACCTGGATACGGCACCGGTTACTTCCAGTACGGTCTCCCGGACCTGGTG
  CCCGACCCCTACTACATCCAGGCTTCCACGTACGTCCAGAAGATGTCTATGTACAACCTGAGATGCGCTG
  CGGAAGAAAACTGCCTGGCCAGTTCAGCATATAGGGCGGATGTCAGAGACTATGACCACAGGGTACTGCT
  ACGATTTCCGCAAAGAGTGAAGAACCAAGGGACATCGGACTTCTTACCAAGCCGCCCTCGGTACTCCTGG
  GAGTGGCACAGCTGTCACCAACATTACCACAGCATGGACGAATTCAGCCACTATGACCTGCTTGATGCCA
  ACACACAGAGGAGAGTGGCTGAAGGCCACAAAGCAAGCTTCTGTCTGGAGGACACGTCCTGTGACTATGG
  GTACCACAGGCGCTTTGCGTGCACTGCACACACACAGGGATTGAGTCCTGGATGTTATGACACCTATGCG
  GCAGACATAGACTGCCAGTGGATTGATATTACAGATGTACAACCTGGAAACTACATTCTAAAGGTCAGTG
  TAAACCCCAGCTACCTGGTGCCTGAATCAGACTACACTAACAATGTTGTACGCTGTGACATTCGCTACAC
  AGGACATCATGCCTATGCCTCAGGCTGCACAATTTCACCGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229764 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRFAWAVLLLGPLQLCPLLRCAPQTPREPPAAPGAWRQTIQWENNGQVFSLLSLGAQYQPQRRRDPSATA
  RRPDGDAASQPRTPILLLRDNRTASTRARTPSPSGVAAGRPRPAARHWFQAGFSPSGARDGASRRAANRT
  ASPQPPQLSNLRPPSHIDRMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGSRNRPGYGTGYFQYGLPDLV
  PDPYYIQASTYVQKMSMYNLRCAAEENCLASSAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSW
  EWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYA
  ADIDCQWIDITDVQPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286182
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286182.1, NP_001273111.1
• RefSeq Size: 4494 bp
• RefSeq ORF: 1236 bp
• Locus ID: 16948
• UniProt ID: P28301
• Cytogenetics: 18 28.22 cM
• MW: 46.7 kDa
• Gene Summary: This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]