Fen1 (NM_001271614) Mouse Tagged ORF Clone

CAT#: MR229610

Fen1 (myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), transcript variant 2

Product Images

Circular map for MR229610

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fen1
• Synonyms: AW538437; FEN-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229610 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATTCACGGCCTTGCCAAACTAATTGCTGATGTGGCCCCCAGTGCCATCCGTGAGAATGACATCA
  AGAGCTACTTTGGTCGCAAAGTGGCCATCGATGCCTCCATGAGCATCTACCAGTTCCTGATTGCTGTTCG
  TCAGGGTGGGGATGTGCTGCAGAACGAGGAGGGTGAGACCACCAGCCACCTGATGGGCATGTTCTACCGT
  ACCATCCGCATGATGGAGAATGGCATCAAGCCTGTGTACGTCTTTGATGGCAAACCACCACAGCTGAAGT
  CAGGCGAGCTGGCCAAGCGCAGTGAGAGGCGCGCCGAGGCTGAGAAGCAACTGCAGCAGGCTCAGGAGGC
  TGGGATGGAGGAGGAGGTGGAGAAGTTCACCAAGAGGCTCGTGAAGGTCACCAAGCAACACAATGATGAG
  TGCAAACACCTGCTGAGCCTCATGGGCATCCCTTACCTTGATGCACCCAGCGAGGCAGAGGCCAGCTGTG
  CTGCCCTGGCAAAGGCTGGCAAAGTCTATGCTGCGGCCACGGAGGACATGGACTGCCTCACTTTTGGCAG
  CCCCGTGCTAATGCGACACTTAACTGCCAGTGAGGCCAAGAAGCTGCCCATCCAAGAGTTCCATCTGAGC
  CGCGTCCTGCAGGAGCTGGGTCTGAACCAGGAGCAGTTTGTGGATCTGTGCATCCTGCTGGGTAGCGACT
  ACTGCGAGAGCATCCGTGGCATTGGGCCCAAGCGGGCTGTGGATCTCATCCAGAAACATAAGAGCATCGA
  GGAGATCGTGAGGCGGCTGGACCCCAGCAAGTACCCCGTTCCAGAGAACTGGCTCCACAAGGAAGCCCAG
  CAGCTCTTCCTGGAGCCAGAAGTACTGGACCCAGAGTCTGTGGAGCTGAAGTGGAGCGAGCCAAATGAAG
  AAGAGTTGGTCAAATTTATGTGTGGTGAAAAGCAGTTTTCTGAAGAGCGAATTCGCAGTGGGGTCAAGCG
  GCTGAGTAAGAGCCGCCAGGGCAGCACCCAGGGACGCCTCGATGATTTCTTCAAGGTGACAGGCTCACTC
  TCCTCAGCTAAGCGCAAGGAGCCAGAACCCAAGGGGCCTGCTAAGAAGAAAGCAAAGACTGGGGGAGCGG
  GGAAGTTCCGAAGGGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229610 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGIHGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYR
  TIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQEAGMEEEVEKFTKRLVKVTKQHNDE
  CKHLLSLMGIPYLDAPSEAEASCAALAKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLS
  RVLQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPSKYPVPENWLHKEAQ
  QLFLEPEVLDPESVELKWSEPNEEELVKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSL
  SSAKRKEPEPKGPAKKKAKTGGAGKFRRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271614
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271614.2
• RefSeq Size: 2211 bp
• RefSeq ORF: 1143 bp
• Locus ID: 14156
• Cytogenetics: 19 A
• MW: 42.6 kDa