Wnt5a (NM_001256224) Mouse Tagged ORF Clone

CAT#: MR229485

Wnt5a (myc-DDK-tagged) - Mouse wingless-type MMTV integration site family, member 5A (Wnt5a), transcript variant 2

Product Images

Circular map for MR229485

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt5a
• Synonyms: 8030457G12Rik; Wnt-5a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229485 representing NM_001256224
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAAGTTCTTCCTAATGGCTTTGGCCACGTTTTTCTCCTTCGCCCAGGTTGTTATAGAAGCTA
  ATTCTTGGTGGTCTCTAGGTATGAATAACCCTGTTCAGATGTCAGAAGTATATATCATAGGTGCACAGCC
  TCTCTGCAGCCAACTGGCAGGACTTTCTCAAGGACAGAAGAAACTCTGCCACTTGTATCAGGACCACATG
  CAGTACATTGGAGAAGGTGCGAAGACAGGCATCAAGGAATGCCAGTACCAGTTCCGGCATCGGAGATGGA
  ACTGCAGCACAGTGGACAATACTTCTGTCTTTGGCAGGGTGATGCAAATAGGCAGCCGAGAGACGGCCTT
  CACGTACGCGGTGAGCGCAGCTGGGGTGGTGAACGCCATGAGCCGAGCATGCCGGGAGGGCGAGCTGTCT
  ACCTGTGGCTGCAGCCGCGCTGCGCGCCCCAAGGACCTGCCTCGGGACTGGTTGTGGGGCGGCTGCGGAG
  ACAACATCGACTATGGCTACCGCTTCGCCAAGGAGTTCGTGGACGCTAGAGAAAGGGAACGAATCCACGC
  TAAGGGTTCCTATGAGAGCGCACGCATCCTCATGAACTTACACAACAATGAAGCAGGCCGTAGGACAGTA
  TACAACCTGGCAGATGTAGCCTGTAAGTGTCATGGAGTGTCTGGCTCCTGTAGCCTCAAGACGTGCTGGC
  TGCAGCTGGCGGACTTCCGGAAGGTGGGCGATGCCCTCAAGGAGAAGTATGATAGCGCGGCGGCCATGAG
  GCTCAACAGCCGGGGCAAGCTGGTGCAGGTCAACAGCCGCTTCAACTCCCCGACCACGCAGGACCTGGTC
  TACATCGACCCCAGTCCGGACTACTGTGTGCGCAACGAGAGCACTGGCTCGCTGGGCACGCAGGGACGCC
  TGTGCAACAAGACCTCAGAGGGGATGGACGGCTGCGAGCTCATGTGCTGTGGGCGTGGCTATGACCAGTT
  TAAGACAGTGCAGACCGAACGCTGTCATTGCAAGTTTCACTGGTGCTGCTATGTCAAATGCAAGAAGTGC
  ACGGAGATTGTGGATCAGTTCGTGTGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229485 representing NM_001256224
  Red=Cloning site Green=Tags(s)
  
  MSSKFFLMALATFFSFAQVVIEANSWWSLGMNNPVQMSEVYIIGAQPLCSQLAGLSQGQKKLCHLYQDHM
  QYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELS
  TCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTV
  YNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLV
  YIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKC
  TEIVDQFVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256224
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256224.2
• RefSeq Size: 3731 bp
• RefSeq ORF: 1083 bp
• Locus ID: 22418
• UniProt ID: P22725
• Cytogenetics: 14 16.8 cM
• MW: 40.9 kDa
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors (PubMed:17117926). Can activate or inhibit canonical Wnt signaling, depending on receptor context (PubMed:16602827). In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (PubMed:16602827). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration (PubMed:17117926). Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells (By similarity). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle (PubMed:10021340). Inhibits type II collagen expression in chondrocytes (By similarity).[UniProtKB/Swiss-Prot Function]