Npr3 (NM_001286395) Mouse Tagged ORF Clone

CAT#: MR228970

Npr3 (myc-DDK-tagged) - Mouse natriuretic peptide receptor 3 (Npr3), transcript variant 3

Product Images

Circular map for MR228970

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Npr3
• Synonyms: ANP-C; ANPR-C; EF-2; lgj; NPR-C; stri
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228970 representing NM_001286395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGCCAGTGGTGACACCATTCGGAGAATCATGTTGGCGGTGCACAGACACGGCATGACCAGTGGAG
  ACTACGCTTTCTTCAACATTGAACTCTTCAACAGTTCTTCCTACGGAGATGGCTCGTGGAGGAGAGGAGA
  CAAACACGACTCTGAAGCTAAACAAGCATACTCGTCCCTCCAAACAGTCACCCTACTGAGGACCGTGAAA
  CCTGAGTTTGAGAAGTTTTCCATGGAGGTGAAAAGTTCTGTTGAGAAACAAGGGCTCAATGAGGAGGATT
  ACGTGAACATGTTTGTTGAAGGGTTCCATGACGCCATCCTCCTCTACGTTCTGGCTTTACATGAAGTACT
  CAGAGCTGGCTACAGCAAGAAGGATGGGGGGAAAATCATCCAGCAGACTTGGAACAGGACATTTGAAGGT
  ATCGCCGGGCAGGTGTCCATAGATGCCAACGGGGACCGGTATGGGGACTTCTCTGTGGTTGCCATGACTG
  ACACTGAAGCAGGCACCCAAGAGGTCATTGGTGATTACTTTGGGAAAGAAGGCCGGTTCCAAATGCGATC
  GAATGTCAAATATCCTTGGGGCCCTTTGAAACTGAGACTAGATGAGACCAGAATCGTGGAGCATACCAAC
  AGCTCTCCTTGCAAATCATGTGGCCTAGAAGAATCTGCAGTGACAGGAATCGTTGTGGGGGCCCTACTAG
  GTGCTGGCTTGCTAATGGCCTTCTACTTTTTCAGGAAGAAATACAGAATAACCATTGAGAGGCGAAATCA
  GCAAGAGGAAAGCAACATCGGGAAGCATCGAGAGCTGCGAGAAGATTCCATCAGATCACATTTTTCGGTG
  GCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228970 representing NM_001286395
  Red=Cloning site Green=Tags(s)
  
  MCASGDTIRRIMLAVHRHGMTSGDYAFFNIELFNSSSYGDGSWRRGDKHDSEAKQAYSSLQTVTLLRTVK
  PEFEKFSMEVKSSVEKQGLNEEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEG
  IAGQVSIDANGDRYGDFSVVAMTDTEAGTQEVIGDYFGKEGRFQMRSNVKYPWGPLKLRLDETRIVEHTN
  SSPCKSCGLEESAVTGIVVGALLGAGLLMAFYFFRKKYRITIERRNQQEESNIGKHRELREDSIRSHFSV
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286395
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286395.1, NP_001273324.1
• RefSeq Size: 6540 bp
• RefSeq ORF: 846 bp
• Locus ID: 18162
• Cytogenetics: 15 5.83 cM
• MW: 32.2 kDa
• Gene Summary: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP (PubMed:10377427, PubMed:17951249). May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects (PubMed:10377427, PubMed:17951249). May regulate diuresis, blood pressure and skeletal development (PubMed:10377427). Does not have guanylate cyclase activity (PubMed:10377427).[UniProtKB/Swiss-Prot Function]