Phf23 (NM_001291126) Mouse Tagged ORF Clone

CAT#: MR228922

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  • TrueORF®

Phf23 (myc-DDK-tagged) - Mouse PHD finger protein 23 (Phf23), transcript variant 3



  "NM_001291126" in other vectors (1)

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USD 317.00


Availability*
2 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Phf23 (NM_001291126) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Phf23
Synonyms JUNE-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR228922 representing NM_001291126
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTCAAGGACTCTCTTTTTGATCTGGATGGGCCCAAAGTGGCATCTCCACTCTCTCCCACATCAT
TGACGCATACTTCCCGGCCCCCCGCTGCTCTCGCCCCAGTGCCGCTGTCCCAGGGGGACCTCTCCCAGCC
TCGAAAGAAGGACCGAAAGAATAGAAAGTTGGGACCAGGAGGTGGGGCTGGCTTTGGGGTGCTTCGGAGA
CCTCGACCAGCTCCTGGGGATGGGGAAAAGCGGTCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTGAAAA
AGGCAGATCGGGGAGATAGACTCCCACCTCCTGGCCCTCCTAGGGCTCCTCCCAGTGATACAGACTCTGA
AGAGGAGGAGGAAGAGGAAGAAGAGGAAGATGATGAGGAAGAGATGACAGTGGGGGGTGGAGTCCCAGCC
CCTGTGCTCCCAACACCCCCTGAGGCCCCCAGGCCCCCTGTTACAGTGCACTCTGAAGGTGCTCCCCCTA
CTGACAGTGAAGGCAAAGATGTGGGCAGCACAGAAACAAGCCAAGATGGAGATGCTAGCTCCAGTGAAGG
CGAGATGCGGGTCATGGATGAGGACATCATGGTAGAATCAGGTGACGACTCCTGGGATCTGATCACGTGT
TACTGCAGAAAGCCCTTTGCGGGGCGGCCCATGATTGAGTGCAGCCTCTGTGGGACGTGGATCCACCTCT
CCTGTGCTAAGATTAAGAAGACCAACGTTCCCGACTTCTTTTATTGCCAGAAATGCAAGGAACTTCGGCC
AGAGGCCCGGCGGTTAGGGGGTCTCCCCAAATCTGGAGAACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR228922 representing NM_001291126
Red=Cloning site Green=Tags(s)

MKLKDSLFDLDGPKVASPLSPTSLTHTSRPPAALAPVPLSQGDLSQPRKKDRKNRKLGPGGGAGFGVLRR
PRPAPGDGEKRSRIKKSKKRKLKKADRGDRLPPPGPPRAPPSDTDSEEEEEEEEEEDDEEEMTVGGGVPA
PVLPTPPEAPRPPVTVHSEGAPPTDSEGKDVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITC
YCRKPFAGRPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGLPKSGEP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001291126
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001291126.1, NP_001278055.1
RefSeq Size 1850 bp
RefSeq ORF 816 bp
Locus ID 78246
UniProt ID Q8BSN5
Cytogenetics 11
MW 29.8 kDa
Gene Summary Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function]

Other Versions

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Frequently bought together (4)
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Customer Reviews 
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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