Rgr (NM_001301694) Mouse Tagged ORF Clone

CAT#: MR228343

Rgr (myc-DDK-tagged) - Mouse retinal G protein coupled receptor (Rgr), transcript variant 3

Product Images

Circular map for MR228343

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rgr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228343 representing NM_001301694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTGTTGCAGCGGTATCCAGCCTCCTCCGGCAGACAGTTGGCATGGGACACGGCCATCCCTCTGG
  TGCTGTTTGTGTGGATGTCATCTGCCTTCTGGGCATCCCTGCCCCTGATGGGCTGGGGCCACTATGACTA
  TGAGCCTGTGGGGACATGCTGTACACTGGACTATTCGAGGGGTGACAGAAACTTCATCAGTTTTCTCTTC
  ACCATGGCTTTTTTCAACTTCCTCGTACCCCTGTTCATCACACACACTTCATACCGGTTCATGGAGCAGA
  AATTCTCCAGGAGTGGCCATCTCCCGGTGAATACTACTCTTCCAGGCAGAATGCTGCTGCTTGGCTGGGG
  CCCCTATGCCCTCCTGTACCTATACGCAGCCATCGCAGATGTGAGCTTCATCTCTCCTAAACTACAGATG
  GTGCCTGCTCTCATCGCCAAAACCATGCCCACAATCAACGCCATCAACTATGCGTTGCACAGGGAGATGG
  TCTGTAGAGGAACCTGGCAGTGTCTGTCTCCACAAAAAAGCAAGAAGGACCGAACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228343 representing NM_001301694
  Red=Cloning site Green=Tags(s)
  
  MPLLQRYPASSGRQLAWDTAIPLVLFVWMSSAFWASLPLMGWGHYDYEPVGTCCTLDYSRGDRNFISFLF
  TMAFFNFLVPLFITHTSYRFMEQKFSRSGHLPVNTTLPGRMLLLGWGPYALLYLYAAIADVSFISPKLQM
  VPALIAKTMPTINAINYALHREMVCRGTWQCLSPQKSKKDRTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301694
• ORF Size: 549 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301694.1, NP_001288623.1
• RefSeq Size: 4135 bp
• RefSeq ORF: 552 bp
• Locus ID: 57811
• Cytogenetics: 14 B
• MW: 21.4 kDa
• Gene Summary: The gene is a member of the opsin family of G-protein coupled receptors. The encoded protein is expressed in the retina, and acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. Disruption of a similar gene in human is associated with autosomal recessive (arRP) and autosomal dominant retinitis pigmentosa (adRP). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]