Vps25 (NM_001284414) Mouse Tagged ORF Clone

CAT#: MR228135

Vps25 (myc-DDK-tagged) - Mouse vacuolar protein sorting 25 (yeast) (Vps25), transcript variant 4

Product Images

Circular map for MR228135

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vps25
• Synonyms: 1110020N13Rik; AW107467; D11Wsu68e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228135 representing NM_001284414
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTACCGCTTCCCGCCCTTCTTTACGTTACAGCCGAACGTGG
  ACACCCGGCAGAAGCAGCTGGCCGCCTGGTGCTCTCTGGTTCTGTCCTTCTGCCGCCTGCACAAACAGTC
  CAGCATGACGGTGATGGAAGCCCAGGAGAGCCCGCTTTTCAACAACGTCAAGCTACAGCGGAAACTTCCT
  GTGGAGTCAATTCAGATTGTATTAGAAGAACTGAGAAAGAAAGGGAACCTCGAGTGGTTGGATAAGAATA
  AGTCTAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATTTACCAATGGGTTTCCAG
  GAGTGGCCAGAATAACTCTGTGTTTACTCTGTATGAACTGACGAGTGGGGAAGACACAGAGGATGAAGCG
  AGCTCAAGATTTGTGGGTGTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228135 representing NM_001284414
  Red=Cloning site Green=Tags(s)
  
  MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP
  VESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTSGEDTEDEA
  SSRFVGVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284414
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284414.1, NP_001271343.1
• RefSeq Size: 613 bp
• RefSeq ORF: 447 bp
• Locus ID: 28084
• UniProt ID: Q9CQ80
• Cytogenetics: 11 64.48 cM
• MW: 17.9 kDa
• Gene Summary: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL (By similarity).[UniProtKB/Swiss-Prot Function]