Arhgdib (NM_001301309) Mouse Tagged ORF Clone

CAT#: MR227931

Arhgdib (myc-DDK-tagged) - Mouse Rho, GDP dissociation inhibitor (GDI) beta (Arhgdib), transcript variant 6

Product Images

Circular map for MR227931

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgdib
• Synonyms: D4; Gdid; Gdid4; Ly-G; Ly-GDI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227931 representing NM_001301309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTTACTGGCGATCTCGAGGCCCTCAAAAAGGATACATTTGTGCTAAAGGAAGGCATTGAATACA
  GGGTGAAAATTAACTTCAAAGTGAATAAGGATATTGTGTCTGGCCTGAAGTATGTTCAACACACATACCG
  GACTGGCATGAGAGTGGATAAAGCCACATTCATGGTTGGCAGCTATGGGCCCCGACCAGAGGAGTACGAA
  TTCCTCACTCCAGTAGAGGAAGCTCCCAAGGGCATGCTGGCCCGAGGCACTTACCACAACAAGTCCTTCT
  TCACGGATGACGACAAACAGGACCACCTCACCTGGGAATGGAACCTGGCCATTAAGAAGGATTGGACAGA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227931 representing NM_001301309
  Red=Cloning site Green=Tags(s)
  
  MDLTGDLEALKKDTFVLKEGIEYRVKINFKVNKDIVSGLKYVQHTYRTGMRVDKATFMVGSYGPRPEEYE
  FLTPVEEAPKGMLARGTYHNKSFFTDDDKQDHLTWEWNLAIKKDWTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301309
• ORF Size: 351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301309.1, NP_001288238.1
• RefSeq Size: 993 bp
• RefSeq ORF: 354 bp
• Locus ID: 11857
• UniProt ID: Q61599
• Cytogenetics: 6 G1
• MW: 14.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the Rho guanine nucleotide dissociation inhibitor (GDI) family. This gene is expressed at high levels in hematopoietic cells. This protein is cytosolic, and dissociation of Rho from this protein is required for membrane association and activation of Rho by Guanine Nucleotide Exchange Factors (GEFs). C-terminal truncations of this gene product have been reported to promote metastasis. Multiple transcript variants and protein isoforms exist. [provided by RefSeq, Aug 2014]