Abcb1a (NM_011076) Mouse Tagged ORF Clone

CAT#: MR227524

Abcb1a (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb1a
• Synonyms: Abcb4; Evi32; mdr-3; Mdr1a; Mdr3; P-gp; Pgp; Pgy-3; Pgy3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227524 representing NM_011076, codon optimized.
  Due to the complexity of NM_011076, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGGAGGAGGACCTCAAGGGCCGAGCGGACAAAAATTTTAGTAAGATGGGCAAGAAATCCAAGA
  AAGAGAAGAAGGAAAAAAAGCCAGCCGTGTCTGTGCTCACAATGTTCCGATACGCAGGGTGGCTTGATCG
  GCTCTACATGCTCGTCGGAACGTTGGCTGCCATCATCCACGGAGTAGCGCTGCCTTTGATGATGTTGATT
  TTCGGTGACATGACCGACTCATTCGCATCCGTTGGGAACGTGTCTAAGAACAGCACAAACATGAGTGAGG
  CTGACAAAAGGGCAATGTTCGCAAAGCTTGAAGAGGAGATGACGACCTACGCATACTACTACACAGGTAT
  CGGCGCAGGGGTCCTCATCGTGGCCTACATCCAGGTGAGTTTCTGGTGTCTGGCCGCTGGCAGACAAATT
  CACAAGATCAGACAAAAATTCTTTCACGCTATTATGAATCAGGAGATTGGATGGTTTGATGTCCACGATG
  TCGGAGAGCTGAACACCCGGCTGACCGACGATGTTAGCAAGATTAATGAAGGTATCGGAGACAAGATCGG
  CATGTTTTTCCAGGCTATGGCGACCTTCTTCGGCGGTTTTATTATAGGATTCACCCGGGGTTGGAAACTC
  ACTCTGGTGATACTTGCTATCAGTCCAGTGCTCGGACTGTCTGCTGGCATATGGGCCAAAATTCTCAGCT
  CATTCACTGACAAAGAACTGCACGCATATGCCAAAGCAGGTGCAGTGGCAGAGGAAGTACTCGCTGCCAT
  TCGCACCGTCATAGCCTTTGGGGGTCAGAAGAAGGAGCTGGAAAGGTACAACAATAACCTGGAAGAGGCT
  AAGAGGCTCGGAATAAAAAAGGCCATTACCGCCAACATCAGCATGGGAGCGGCATTCCTTCTCATCTACG
  CCTCTTACGCCCTGGCCTTCTGGTACGGAACTAGCCTGGTCATTTCCAAAGAATACAGCATCGGGCAGGT
  GCTTACCGTGTTCTTCTCCGTCCTCATCGGCGCTTTCTCCGTCGGTCAGGCCAGCCCCAATATCGAGGCA
  TTCGCTAATGCCAGAGGAGCCGCATATGAAGTATTTAAGATCATTGACAACAAGCCATCCATCGACTCTT
  TTTCTAAATCTGGACACAAGCCAGATAACATCCAAGGGAACCTGGAATTTAAAAATATTCACTTCAGTTA
  TCCCTCCCGGAAGGAGGTCCAGATCCTTAAAGGACTCAATCTTAAAGTCAAATCCGGCCAGACTGTAGCA
  CTCGTCGGTAATTCTGGGTGTGGGAAGAGTACCACAGTGCAGTTGATGCAGCGCCTCTATGACCCCTTGG
  ACGGGATGGTCAGCATAGACGGGCAAGATATTAGGACAATCAACGTTCGATATCTGCGAGAAATTATCGG
  TGTCGTGAGCCAAGAACCAGTCCTCTTCGCCACTACTATTGCAGAGAACATCAGATATGGCCGAGAGGAT
  GTCACAATGGACGAGATTGAGAAGGCTGTCAAAGAGGCTAATGCTTACGATTTCATCATGAAGCTGCCTC
  ACCAGTTTGACACACTCGTTGGTGAGAGGGGCGCTCAATTGTCCGGCGGACAGAAGCAAAGGATCGCAAT
  TGCGCGGGCGCTCGTGCGCAACCCAAAAATATTGCTGTTGGACGAAGCCACCTCTGCACTGGACACCGAG
  TCCGAGGCCGTGGTGCAGGCTGCCCTTGATAAGGCTCGGGAGGGCCGAACCACTATTGTCATTGCACACC
  GCCTCTCCACAGTGAGAAACGCCGACGTCATCGCAGGGTTCGATGGCGGCGTCATTGTGGAACAGGGCAA
  CCACGATGAACTCATGCGCGAGAAGGGAATATATTTTAAACTGGTGATGACCCAGACCGCGGGCAATGAG
  ATCGAACTGGGAAACGAGGCCTGTAAGAGCAAGGATGAAATTGACAACCTGGACATGTCTAGCAAAGACT
  CCGGATCTTCTTTGATTAGGAGGCGCTCCACGCGGAAGTCCATTTGTGGACCACATGATCAAGACCGCAA
  GCTCTCCACCAAGGAGGCACTGGACGAGGACGTTCCTCCTGCCTCATTCTGGCGGATTCTGAAATTGAAC
  AGCACAGAATGGCCATACTTTGTTGTGGGAATCTTTTGCGCTATTATTAACGGGGGGCTCCAACCAGCAT
  TCTCCGTCATTTTCTCCAAGGTGGTGGGCGTCTTCACTAATGGTGGACCACCAGAAACTCAAAGACAGAA
  TAGTAACTTGTTTTCACTTCTCTTCCTTATACTCGGCATTATTAGCTTTATTACGTTTTTCCTGCAGGGG
  TTTACATTTGGCAAGGCTGGAGAGATTCTGACAAAGCGCCTGAGATACATGGTCTTTAAGTCAATGCTTA
  GGCAGGACGTCAGCTGGTTCGACGATCCCAAAAATACTACCGGAGCTCTTACCACTCGCCTTGCCAATGA
  CGCCGCTCAGGTGAAGGGAGCTACAGGCAGTCGACTTGCTGTAATCTTCCAGAACATCGCTAACTTGGGG
  ACTGGAATCATCATATCCTTGATATATGGCTGGCAACTTACATTGCTTCTTCTCGCCATTGTTCCTATTA
  TTGCAATCGCTGGAGTCGTTGAGATGAAAATGCTGTCAGGGCAGGCCCTGAAAGACAAAAAGGAATTGGA
  GGGTAGTGGCAAAATTGCCACAGAAGCAATTGAAAACTTCAGAACTGTTGTCTCTTTGACGAGAGAACAA
  AAATTCGAAACAATGTATGCCCAGTCTTTGCAGATCCCATACAGGAACGCAATGAAAAAGGCCCACGTGT
  TCGGTATCACTTTTTCTTTTACACAGGCAATGATGTATTTCTCTTACGCTGCCTGTTTTAGATTCGGCGC
  GTATCTTGTGACGCAGCAGCTGATGACTTTCGAGAATGTCCTTCTTGTATTTAGTGCCATCGTGTTTGGG
  GCAATGGCGGTCGGGCAGGTAAGCTCCTTCGCCCCTGACTATGCTAAGGCAACGGTGAGCGCTAGCCATA
  TCATCAGGATCATAGAGAAGACTCCCGAGATAGATTCCTATTCTACCCAGGGGCTGAAGCCTAACATGCT
  GGAAGGCAACGTCCAATTTTCTGGGGTTGTGTTCAATTATCCGACTCGACCCTCCATTCCCGTACTGCAA
  GGCCTCTCTCTCGAGGTCAAAAAGGGCCAAACTCTCGCCTTGGTTGGCTCATCTGGATGTGGCAAGAGCA
  CTGTTGTGCAATTGCTGGAGCGATTTTATGATCCAATGGCTGGCTCAGTGTTTCTGGACGGCAAGGAGAT
  CAAGCAGCTCAACGTGCAGTGGCTGCGCGCCCAGCTTGGGATAGTCAGTCAGGAACCAATCCTGTTTGAC
  TGCTCAATAGCTGAGAACATTGCCTACGGGGACAATAGCCGGGTCGTTTCATACGAGGAAATTGTTAGGG
  CTGCGAAAGAGGCTAACATTCATCAATTTATCGACTCCCTGCCTGACAAGTATAATACAAGGGTTGGAGA
  TAAAGGTACACAGCTGTCAGGTGGTCAGAAGCAGCGGATCGCTATTGCTCGCGCTCTGGTTCGACAGCCA
  CACATCTTGCTTTTGGATGAAGCCACCTCAGCATTGGATACCGAGTCCGAGAAAGTCGTGCAGGAGGCTC
  TGGATAAGGCTAGAGAAGGAAGGACATGTATCGTAATCGCACATCGGCTTTCCACTATCCAGAACGCAGA
  CCTGATAGTGGTCATCCAGAACGGAAAAGTCAAGGAACATGGGACCCACCAGCAGCTTCTTGCTCAGAAG
  GGAATTTACTTCTCCATGGTGTCCGTCCAGGCAGGCGCTAAGCGGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227524 representing NM_011076
  Red=Cloning site Green=Tags(s)
  
  MELEEDLKGRADKNFSKMGKKSKKEKKEKKPAVSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLI
  FGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQI
  HKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKL
  TLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEA
  KRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEA
  FANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVA
  LVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED
  VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTE
  SEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTAGNE
  IELGNEACKSKDEIDNLDMSSKDSGSSLIRRRSTRKSICGPHDQDRKLSTKEALDEDVPPASFWRILKLN
  STEWPYFVVGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQNSNLFSLLFLILGIISFITFFLQG
  FTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLG
  TGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQ
  KFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFG
  AMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQ
  GLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFD
  CSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQP
  HILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQK
  GIYFSMVSVQAGAKRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011076
• ORF Size: 3828 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011076.1, NM_011076.2, NP_035206.2
• RefSeq Size: 5177 bp
• RefSeq ORF: 3831 bp
• Locus ID: 18671
• UniProt ID: P21447
• Cytogenetics: 5 3.43 cM
• MW: 140.6 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a p-glycoprotein which actively transports a variety of hydrophobic amphipathic drugs and plays a major role in the blood-brain barrier permeability of certain drugs. [provided by RefSeq, Jul 2008]