Cacna1g (NM_009783) Mouse Tagged ORF Clone

CAT#: MR227520

Cacna1g (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 1

Product Images

Circular map for MR227520

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1g
• Synonyms: a1G; alpha-1G; Cav3.1d; mKIAA1123; [a]1G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227520 representing NM_009783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGAAGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCACGCAGCTCAACG
  ACCTGTCTGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCCTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGCATGCTGGTTATTCTCCTCA
  ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAACGCTGCCGGATCCT
  GCAGGCCTTCGACGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTCGCTTTGGGT
  ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTTATCGTCATTGCTGGGA
  TGCTGGAGTACTCGCTGGACCTGCAGAATGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGATACCTTGCCTATG
  CTGGGCAATGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTTGGTGTTCAGCTGTGGG
  CAGGGCTACTTCGAAACCGATGCTTCCTCCCTGAGAATTTCAGCCTCCCGCTGAGTGTGGACTTGGAGCC
  TTACTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAACGGCATGCGA
  TCCTGCAGGAGCGTGCCCACACTGCGCGGGGAAGGCGGTGGTGGTCCACCCTGCGGTCTGGACTATGAGG
  CCTATAACAGTTCCAGCAATACCACCTGTGTCAACTGGAACCAATACTATACCAACTGCTCTGCAGGCGA
  GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACACTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAACTTCATCT
  ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATCGCCACACA
  GTTCTCTGAGACCAAGCAACGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT
  AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTTCTCAAGTACCTGGTGTACATCC
  TCCGCAAAGCAGCCCGCAGGCTGGCCCAGGTCTCTAGGGCTGTAGGCGTGCGGGCTGGGTTGCTCAGCAG
  CCCAGTGGCCCGTGGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCTCTCGTTCACACCGTCGTCTGTCT
  GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG
  TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCCCGCTGGCTCATGCTGCCACCACC
  CTCTACCCCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC
  TGCCACTTGGAGCCAGTCCGTTGCCAGGCGCCCCCTCCCAGGTCCCCATCGGAGGCATCTGGCAGGACTG
  TGGGTAGTGGGAAGGTATACCCCACTGTGCATACCAGCCCTCCACCAGAGATGCTGAAGGATAAGGCACT
  AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC
  TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT
  GCTCCAAGGCAGACAGTGGAGCCTGTGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGGGA
  GCCAGAGTCCGCTGACCATGAAATGCCTGACTCAGACAGTGAGGCTGTGTATGAGTTCACACAGGACGCT
  CAGCACAGCGACCTCCGGGATCCCCACAGACGGCGACGGCCGAGCCTGGGCCCAGATGCAGAGCCTAGTT
  CTGTGCTGGCCTTCTGGAGGCTGATCTGCGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGGCCG
  GGGAATCATGATCGCTATCCTGGTCAATACCCTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAGGAG
  CTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGAAGC
  TGCTCGTCTACGGTCCCTTCGGCTACATTAAGAATCCCTACAACATCTTTGATGGCGTCATTGTGGTCAT
  CAGCGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATGCGG
  GTGCTGAAGCTAGTTCGCTTCCTGCCGGCACTGCAGCGGCAGCTCGTGGTGCTCATGAAGACCATGGACA
  ACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCACCTTTT
  TGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTTGACTCCCTGCTC
  TGGGCCATTGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTTTACAACGGCATGG
  CCTCCACGTCATCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTACGTGCTCTTTAA
  CCTGCTTGTCGCCATTCTCGTGGAGGGTTTCCAGGCAGAGGAAATCGGCAAACGGGAAGATACGAGTGGA
  CAGTTAAGCTGTATTCAGCTGCCTGTCAACTCCCAGGGGGGAGATGCCACCAAGTCTGAGTCAGAGCCTG
  ATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGGAAGAAGCGCTTGGCCCTGGTGGCCTTGGGAGA
  ACACTCGGAACTACGAAAGAGCCTTTTGCCACCTCTCATCATCCACACAGCTGCTACACCGATGTCACTG
  CCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGCCGCACCAGTAGCAGTGGGT
  CCGCTGAGCCTGGAACTGCCCATCATGAGATGAAATCACCGCCAAGTGCCCGAAGCTCCCCGCACAGTCC
  CTGGAGCGCAGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCCTGGGCCGGGCCCCCAGCCTG
  AAGCGTAGGAGCCCAAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGTCAGGAGAGCCAGGATGAGG
  AGGAGAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGATCATCGCCACAGGGGTTCCTTGGAACG
  TGAGGCCAAGAGTTCCTTTGACCTGCCTGACACCCTGCAGGTGCCCGGGCTTCATCGAACAGCCAGCGGT
  CGGAGCTCTGCCTCTGAACACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACCCTGC
  GGGCTGATGACCCCCCACTGGATGGGGATGATGGCGATGATGAGGGCAACCTGAGCAAAGGGGAACGCTT
  ACGAGCCTGGGTCCGAGCCCGGCTCCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCTGCCTATATCTTC
  CCTCCCCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTGGTCC
  TCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCGCTGA
  ACGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTCCTGGCTGAAATGACAGTGAAGGTG
  GTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAACGTGCTGGACGGCTTGC
  TGGTGCTCATCTCTGTCATCGACATCCTGGTGTCCATGGTCTCTGACAGCGGCACCAAGATTCTCGGCAT
  GCTGAGGGTGCTGCGGCTGCTGCGGACCCTACGTCCACTCAGGGTCATCAGCCGGGCCCAGGGGCTGAAG
  CTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATCTGCTGTGCCTTCT
  TCATCATTTTTGGAATTCTTGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGGACAC
  CAGGAACATCACTAACAAGTCCGACTGTGCTGAGGCCAGTTACCGGTGGGTCCGGCACAAGTACAACTTT
  GACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGCTGGGTTGACATCATGT
  ATGATGGACTGGATGCTGTGGGAGTGGACCAGCAGCCCATCATGAACCACAACCCTTGGATGCTGCTCTA
  CTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTCGTCCTGAACATGTTTGTGGGCGTGGTGGTGGAGAAC
  TTCCATAAGTGCAGGCAGCACCAGGAGGAGGAGGAGGCGCGGCGGCGGGAGGAGAAGCGACTAAAGAGGC
  TGGAGAAAAAGAGAAGGAGTAAGGAGAAGCAGATGGCCGATCTAATGTTGGACGATGTAATTGCTTCCGG
  CAGCTCAGCCAGCGCTGCGTCAGAAGCCCAGTGCAAACCCTACTACTCTGACTACTCGCGCTTCCGGCTC
  CTCGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGGTGTCATCGGGCTGAATGTGG
  TCACGATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCTCTGAAGATCTGCAACTACAT
  CTTTACCGTCATCTTTGTCTTGGAGTCAGTATTCAAACTTGTGGCCTTCGGCTTCCGCCGGTTCTTCCAG
  GACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCATGGGCATCACGCTGGAAGAGATTG
  AGGTCAATGCTTCACTGCCCATCAACCCCACCATCATCCGTATCATGAGGGTGCTCCGCATTGCTCGAGT
  TCTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACACGGTGATGCAGGCCCTGCCCCAG
  GTGGGGAACCTGGGACTTCTCTTCATGCTATTATTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTG
  GAGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGCCGGCATGCCACCTTTAGGAACTTTGG
  TATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGAATGGTATTATGAAGGACACCCTC
  CGGGACTGTGACCAGGAGTCCACCTGCTACAACACCGTCATCTCACCCATCTACTTCGTGTCCTTCGTGC
  TGACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCCGTGCTGATGAAGCACCTGGAAGAGAGCAACAA
  AGAGGCCAAGGAGGAGGCGGAGTTGGAGGCGGAGCTGGAGCTAGAGATGAAGACACTCAGCCCGCAGCCC
  CACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCTGGGGTGGAAGGTGTCAATAGCCCTGACAGCCCTAAGC
  CTGGGGCTCCACACACCACGGCCCACATTGGAGCAGCCTCTTCAGGCTTCTCCCTTGAGCACCCCACGAT
  GGTACCTCACACTGAGGAGGGGCCAGTCCCCCTAGGACCAGACCTGCTGACTGTGAGGAAGTCTGGTGTC
  AGCCGGACACACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAGCACTGCCGAGAGATCCCTAG
  GACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCTGTTCACTCCCAACCAGCAGA
  CACCAGCTGCATCCTACAGCTTCCCAAAGATGCACACTATCTGCTCCAGCCTCATGGGGCTCCCACCTGG
  GGCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAGGCCTCTCAGGCGCCAGGCAG
  CAATAAGGACTGACTCCCTGGACGTGCAGGGCCTGGGTAGCCGGGAAGACCTGTTGTCAGAGGTGAGTGG
  GCCCTCCTGCCCTCTGACCCGCTCCTCATCCTTCTGGGGCGGGTCGAGCATCCAGGTGCAGCAGCGCTCC
  GGCAGCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCCAGGCCTGGAACCCAGCTGGG
  CCAAGGACCCTCAAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTGAGCTGGATTTCAGGAGACCT
  CCTGCCCAGCAGTCAGGAAGAACCCCTGTCCCCACGGGACTTGAAAAAATGCTACAGTGTAGAGGCCCAG
  AGCTGCCGGCGCAGGCCTGGGTCCTGGCTAGACGAACAGAGGAGACACTCCATCGCTGTCAGCTGCCTGG
  ACAGCGGCTCCCAGCCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGCCAACCTCTTGGGGGCCCTGG
  GAGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCCCGGAGAGCCAGGGCCCTCGG
  CCCCCATGCAGTCCTGGCGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGACTCGAAGGATCCCTCGGCCT
  CCAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGATGCGCTGAGTCTCTCTGGTTTGTC
  TTCTGACCCAACAGACCTGGATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227520 representing NM_009783
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA
  STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLS
  VHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHAD
  CHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFS
  SMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDA
  QHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEE
  LTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMR
  VLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLL
  WAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSG
  QLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSL
  PKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSL
  KRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASG
  RSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIF
  PPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKV
  VALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLK
  LVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNF
  DNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVEN
  FHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRL
  LVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQ
  DRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQ
  VGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTL
  RDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQP
  HSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGV
  SRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTW
  GAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRS
  GSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQ
  SCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPR
  PPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009783
• ORF Size: 6885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009783.3, NP_033913.2
• RefSeq Size: 8238 bp
• RefSeq ORF: 6888 bp
• Locus ID: 12291
• Cytogenetics: 11 D
• MW: 253.9 kDa