Sall4 (NM_175303) Mouse Tagged ORF Clone

CAT#: MR227483

Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a

Product Images

Circular map for MR227483

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sall4
• Synonyms: 5730441M18Rik; AA407717; AL022809; AW536104; C78083; C78563; C330011P20Rik; Tex20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227483 representing NM_175303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGGCGCAAGCAGGCGAAGCCCCAGCACATCAACTGGGAGGAGGGCCAGGGCGAGCAGCCTCAGC
  AGCTACCGAGCCCCGACCTCGCCGAGGCGCTGGCGGCGGAGGAACCCGGTGCTCCAGTGAACTCCCCTGG
  GAACTGCGATGAAGCCTCAGAGGACTCCATACCGGTGAAGCGGCCCCGGCGGGAGGACACTCACATCTGC
  AACAAATGCTGTGCCGAGTTCTTTAGTCTCTCTGAATTCATGGAACACAAGAAAAGTTGCACTAAAACCC
  CTCCTGTCCTCATCATGAATGACAGCGAGGGGCCAGTGCCTTCAGAGGACTTTTCCAGAGCTGCCCTGAG
  CCACCAGCTGGGCAGCCCAAGCAATAAAGACAGTCTCCAGGAGAACGGCAGCAGCTCGGGGGACTTGAAG
  AAGCTGGGCACGGACTCCATCCTGTACTTGAAGACAGAGGCTACCCAGCCATCCACACCCCAGGACATAA
  GCTATTTACCCAAAGGCAAAGTAGCCAACACCAATGTGACTCTGCAGGCGCTCCGCGGCACCAAGGTGGC
  CGTGAACCAACGGGGTGCAGAGGCACCCATGGCGCCCATGCCTGCTGCCCAAGGCATCCCTTGGGTCCTG
  GAGCAGATCCTGTGCCTGCAGCAGCAGCAACTCCAGCAAATCCAGCTTACGGAACAGATTCGCGTCCAGG
  TGAACATGTGGGCAGCGCACGCGCTCCACTCTGGAGTGGCGGGGGCCGACACGCTGAAGGCCTTAAGCAG
  CCATGTGTCTCAGCAAGTGTCCGTGTCCCAGCAGGTGTCGGCTGCCGTGGCCCTGCTCAGCCAGAAAGCC
  TCAAACCCAGCTCTGTCGCTCGATGCCTTGAAACAAGCCAAGCTACCTCATGCCAGCGTCCCCTCCGCAG
  CCAGCCCGTTGTCCTCGGGGTTAACGTCCTTCACCTTGAAGCCTGACGGGACACGGGTTCTCCCCAACTT
  CGTGTCTCGCCTTCCCAGTGCCCTGCTACCTCAGACTCCGGGCTCTGTGCTCCTGCAGAGTCCCTTCTCC
  GCTGTGACGCTCGACCAGTCCAAGAAAGGAAAGGGGAAACCCCAGAACCTCTCCGCCTCTGCCTCGGTGT
  TAGATGTCAAGGCCAAGGACGAAGTCGTCCTCGGTAAGCACAAGTGTAGGTACTGTCCCAAGGTTTTCGG
  GACAGATAGCTCCCTTCAGATTCACCTTCGCTCCCACACCGGAGAGAGACCTTACGTGTGCCCTATCTGT
  GGTCACCGCTTCACCACCAAGGGCAATCTCAAGGTCCACTTACAACGACACCCTGAGGTGAAGGCAAACC
  CCCAGCTGTTGGCCGAATTCCAGGACAAAGGGGCAGTGAGTGCCGCTTCTCACTATGCACTCCCTGTCCC
  CGTCCCTGCCGATGAATCGAGTCTCTCTGTAGACGCCGAGCCTGTCCCGGTCACGGGAACCCCTTCTCTA
  GGGCTACCTCAAAAGCTCACGTCAGGGCCTAATTCCAGGGACCTCATGGGTGGCTCCTTGCCCAATGACA
  TGCAGCCAGGGCCTTCTCCAGAAAGTGAGGCGGGCCTTCCACTCCTTGGGGTGGGGATGATACATAATCC
  CCCAAAGGCTGGGGGCTTCCAGGGCACTGGGGCCCCAGAGTCAGGGTCCGAGACCCTGAAATTGCAGCAA
  CTAGTGGAGAACATAGACAAGGCCACTACTGACCCCAACGAGTGTCTCATTTGTCATCGGGTCCTCAGCT
  GTCAGAGTTCCCTGAAGATGCATTACCGTACCCACACAGGGGAGAGACCATTCCAGTGCAAGATCTGTGG
  CCGGGCCTTCTCCACCAAAGGCAACCTGAAGACACACCTTGGGGTTCACCGAACCAACACGACCGTAAAG
  ACCCAACATTCGTGCCCCATCTGCCAGAAGAAATTCACCAACGCCGTCATGTTACAGCAGCATATCCGGA
  TGCACATGGGTGGCCAGATCCCCAACACCCCTCTGCCAGAGAGTCCCTGTGACTTCACGGCTCCCGAGCC
  CGTGGCCGTCAGTGAGAATGGCAGTGCCAGCGGGGTCTGCCAGGACGACGCAGCAGAAGGGATGGAAGCC
  GAGGAGGTCTGTTCTCAGGATGTTCCCAGTGGCCCCTCAACTGTCTCTCTGCCGGTTCCCAGTGCCCACC
  TGGCATCGCCCTCTCTGGGCTTCTCTGTGTTGGCCTCCCTGGATACGCAGGGGAAAGGGGCTCTTCCGGC
  GCTGGCCCTGCAGAGGCAGAGCAGTCGAGAAAACAGCTCCCTGGAGGGCGGTGACACTGGTCCAGCCAAT
  GACTCTTCCTTGCTCGTGGGTGACCAGGAGTGTCAGAGCCGAAGCCCAGATGCCACGGAGACCATGTGCT
  ACCAGGCAGTGTCACCTGCCAATAGCCAAGCCGGAAGTGTCAAGTCCCGGTCTCCCGAGGGTCACAAGGC
  CGAGGGCGTGGAGAGCTGCCGCGTTGACACCGAAGGTCGTACCAGCCTCCCTCCAACATTTATCCGAGCA
  CAGCCCACCTTTGTCAAAGTTGAAGTGCCTGGCACCTTTGTGGGACCCCCCAGCATGCCCTCGGGTATGC
  CGCCTTTGCTAGCATCGCAGCCGCAGCCACGCCGCCAGGCCAAGCAGCACTGCTGCACACGGTGTGGAAA
  GAACTTCTCGTCTGCCAGTGCCCTGCAGATCCACGAGCGAACACACACGGGAGAGAAGCCTTTCGTGTGT
  AACATATGCGGGCGGGCCTTCACCACGAAAGGCAACCTGAAGGTACACTACATGACTCATGGGGCCAACA
  ATAACTCCGCCCGCCGGGGAAGGAAGCTGGCCATAGAGAACCCCATGGCCGCGCTGAGTGCTGAGGGAAA
  GAGAGCGCCCGAGGTGTTTTCCAAGGAGCTCCTGTCCCCCGCGGTGAGTGTGGACCCCGCCTCCTGGAAC
  CAGTACACCAGCGTCCTGAATGGGGGTCTGGCCATGAAGACCAACGAGATCTCCGTGATCCAGAGCGGAG
  GCATCCCCACGCTGCCTGTGTCGCTGGGGGCCAGCTCTGTGGTGAGCAATGGCACGATTTCCAAGCTTGA
  CGGCTCTCAGACCGGTGTGAGCATGCCCATGAGCGGGAACGGAGAAAAGCTCGCTGTTCCCGACGGCATG
  GCCAAACACCAGTTCCCTCACTTCCTGGAGGAAAATAAGATTGCTGTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227483 representing NM_175303
  Red=Cloning site Green=Tags(s)
  
  MSRRKQAKPQHINWEEGQGEQPQQLPSPDLAEALAAEEPGAPVNSPGNCDEASEDSIPVKRPRREDTHIC
  NKCCAEFFSLSEFMEHKKSCTKTPPVLIMNDSEGPVPSEDFSRAALSHQLGSPSNKDSLQENGSSSGDLK
  KLGTDSILYLKTEATQPSTPQDISYLPKGKVANTNVTLQALRGTKVAVNQRGAEAPMAPMPAAQGIPWVL
  EQILCLQQQQLQQIQLTEQIRVQVNMWAAHALHSGVAGADTLKALSSHVSQQVSVSQQVSAAVALLSQKA
  SNPALSLDALKQAKLPHASVPSAASPLSSGLTSFTLKPDGTRVLPNFVSRLPSALLPQTPGSVLLQSPFS
  AVTLDQSKKGKGKPQNLSASASVLDVKAKDEVVLGKHKCRYCPKVFGTDSSLQIHLRSHTGERPYVCPIC
  GHRFTTKGNLKVHLQRHPEVKANPQLLAEFQDKGAVSAASHYALPVPVPADESSLSVDAEPVPVTGTPSL
  GLPQKLTSGPNSRDLMGGSLPNDMQPGPSPESEAGLPLLGVGMIHNPPKAGGFQGTGAPESGSETLKLQQ
  LVENIDKATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTTVK
  TQHSCPICQKKFTNAVMLQQHIRMHMGGQIPNTPLPESPCDFTAPEPVAVSENGSASGVCQDDAAEGMEA
  EEVCSQDVPSGPSTVSLPVPSAHLASPSLGFSVLASLDTQGKGALPALALQRQSSRENSSLEGGDTGPAN
  DSSLLVGDQECQSRSPDATETMCYQAVSPANSQAGSVKSRSPEGHKAEGVESCRVDTEGRTSLPPTFIRA
  QPTFVKVEVPGTFVGPPSMPSGMPPLLASQPQPRRQAKQHCCTRCGKNFSSASALQIHERTHTGEKPFVC
  NICGRAFTTKGNLKVHYMTHGANNNSARRGRKLAIENPMAALSAEGKRAPEVFSKELLSPAVSVDPASWN
  QYTSVLNGGLAMKTNEISVIQSGGIPTLPVSLGASSVVSNGTISKLDGSQTGVSMPMSGNGEKLAVPDGM
  AKHQFPHFLEENKIAVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175303
• ORF Size: 3201 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175303.4, NP_780512.2
• RefSeq Size: 5073 bp
• RefSeq ORF: 3204 bp
• Locus ID: 99377
• UniProt ID: Q8BX22
• Cytogenetics: 2 88.99 cM
• MW: 113.6 kDa
• Gene Summary: This gene belongs to the spalt family of zinc finger transcription factors. In mouse, functions for this gene have been described in many embryonic developmental processes, including brain, heart, and limb development. In addition, this gene is an important pluripotency factor that is required for stem cell maintenance. Homozygous mutant mice display embryonic lethality, while conditional knock-out in embryonic germ cells results in failure to establish a robust stem cell population. A pseudogene of this gene is found on chromosome 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]