Cdh1 (NM_009864) Mouse Tagged ORF Clone

CAT#: MR227372

Cdh1 (Myc-DDK-tagged) - Mouse cadherin 1 (Cdh1)

Product Images

Circular map for MR227372

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdh1
• Synonyms: AA960649; ARC-1; E-ca; E-cad; E-cadh; Eca; Ecad; L-C; L-CAM; Um; UVO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227372 representing NM_009864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCCCGGTGCCGCAGCTTTTCCGCGCTCCTGCTCCTGCTGCAGGTCTCCTCATGGCTTTGCCAGG
  AGCTGGAGCCTGAGTCCTGCAGTCCCGGCTTCAGTTCCGAGGTCTACACCTTCCCGGTGCCGGAGAGGCA
  CCTGGAGAGAGGCCATGTCCTGGGCAGAGTGAGATTTGAAGGATGCACTGGCCGGCCAAGGACAGCCTTC
  TTTTCGGAAGACTCCCGATTCAAAGTGGCGACAGACGGCACCATCACAGTGAAGCGGCATCTAAAGCTCC
  ACAAGCTGGAGACCAGTTTCCTCGTCCGCGCCCGGGACTCCAGTCATAGGGAGCTGTCTACCAAAGTGAC
  GCTGAAGTCCATGGGGCACCACCATCACCGGCACCACCACCGCGACCCTGCCTCTGAATCCAACCCAGAG
  CTGCTCATGTTTCCCAGCGTGTACCCAGGTCTCAGAAGACAGAAACGAGACTGGGTCATCCCTCCCATCA
  GCTGCCCCGAAAATGAAAAGGGCGAATTTCCAAAGAACCTGGTTCAGATCAAATCCAACAGGGACAAAGA
  AACAAAGGTTTTCTACAGCATCACCGGCCAAGGAGCTGACAAACCCCCCGTTGGCGTTTTCATCATTGAG
  AGGGAGACAGGCTGGCTGAAAGTGACACAGCCTCTGGATAGAGAAGCCATTGCCAAGTACATCCTCTATT
  CTCATGCCGTGTCATCAAATGGGGAAGCGGTGGAGGATCCCATGGAGATAGTGATCACAGTGACAGATCA
  GAATGACAACAGGCCAGAGTTTACCCAGCCGGTCTTTGAGGGATTCGTTGCAGAAGGCGCTGTTCCAGGA
  ACCTCCGTGATGAAGGTCTCAGCCACCGATGCAGACGATGACGTCAACACCTACAACGCTGCCATCGCCT
  ACACCATCGTCAGCCAGGATCCTGAGCTGCCTCACAAAAACATGTTCACTGTCAATAGGGACACCGGGGT
  CATCAGTGTGCTCACCTCTGGGCTGGACCGAGAGAGTTACCCTACATACACTCTGGTGGTTCAGGCTGCT
  GACCTTCAAGGTGAAGGCTTGAGCACAACAGCCAAGGCTGTGATCACTGTCAAGGATATTAATGACAACG
  CTCCTGTCTTCAACCCAAGCACGTATCAGGGTCAAGTGCCTGAGAATGAGGTCAATGCCCGGATCGCCAC
  ACTCAAAGTGACCGATGATGATGCCCCCAACACTCCGGCGTGGAAAGCTGTGTACACCGTAGTCAACGAT
  CCTGACCAGCAGTTCGTTGTTGTCACAGACCCCACGACCAATGATGGCATTTTGAAAACAGCCAAGGGCT
  TGGATTTTGAGGCCAAGCAGCAATACATCCTTCATGTGAGAGTGGAGAACGAGGAACCCTTTGAGGGGTC
  TCTTGTCCCTTCCACAGCCACTGTCACTGTGGACGTGGTAGACGTGAATGAAGCCCCCATCTTTATGCCT
  GCGGAGAGGAGAGTCGAAGTGCCCGAAGACTTTGGTGTGGGTCAGGAAATCACATCTTATACCGCTCGAG
  AGCCGGACACGTTCATGGATCAGAAGATCACGTATCGGATTTGGAGGGACACTGCCAACTGGCTGGAGAT
  TAACCCAGAGACTGGTGCCATTTTCACGCGCGCTGAGATGGACAGAGAAGACGCTGAGCATGTGAAGAAC
  AGCACATATGTAGCTCTCATCATCGCCACAGATGATGGTTCACCCATTGCCACTGGCACGGGCACTCTTC
  TCCTGGTCCTGTTAGACGTCAATGACAACGCTCCCATCCCAGAACCTCGAAACATGCAGTTCTGCCAGAG
  GAACCCACAGCCTCATATCATCACCATCTTGGATCCAGACCTTCCCCCCAACACGTCCCCCTTTACTGCT
  GAGCTAACCCATGGGGCCAGCGTCAACTGGACCATTGAGTATAATGACGCAGCTCAAGAATCTCTCATTT
  TGCAACCAAGAAAGGACTTAGAGATTGGCGAATACAAAATCCATCTCAAGCTCGCGGATAACCAGAACAA
  AGACCAGGTGACCACGTTGGACGTCCATGTGTGTGACTGTGAAGGGACGGTCAACAACTGCATGAAGGCG
  GGAATCGTGGCAGCAGGATTGCAAGTTCCTGCCATCCTCGGAATCCTTGGAGGGATCCTCGCCCTGCTGA
  TTCTGATCCTGCTGCTCCTACTGTTTCTACGGAGGAGAACGGTGGTCAAAGAGCCCCTGCTGCCACCAGA
  TGATGATACCCGGGACAATGTGTATTACTATGATGAAGAAGGAGGTGGAGAAGAAGACCAGGACTTTGAT
  TTGAGCCAGCTGCACAGGGGCCTGGATGCCCGACCGGAAGTGACTCGAAATGATGTGGCTCCCACCCTCA
  TGAGCGTGCCCCAGTATCGTCCCCGTCCTGCCAATCCTGATGAAATTGGAAACTTCATCGATGAAAACCT
  GAAGGCAGCCGACAGCGACCCCACGGCACCCCCTTACGACTCTCTGTTGGTGTTCGATTACGAGGGCAGT
  GGTTCTGAAGCCGCTAGCCTGAGCTCACTGAACTCCTCTGAGTCGGATCAGGACCAGGACTACGATTATC
  TGAACGAGTGGGGCAACCGATTCAAGAAGCTGGCGGACATGTACGGCGGTGGTGAGGACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227372 representing NM_009864
  Red=Cloning site Green=Tags(s)
  
  MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVPERHLERGHVLGRVRFEGCTGRPRTAF
  FSEDSRFKVATDGTITVKRHLKLHKLETSFLVRARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPE
  LLMFPSVYPGLRRQKRDWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIE
  RETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQPVFEGFVAEGAVPG
  TSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAA
  DLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVND
  PDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMP
  AERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKN
  STYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTA
  ELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKA
  GIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVKEPLLPPDDDTRDNVYYYDEEGGGEEDQDFD
  LSQLHRGLDARPEVTRNDVAPTLMSVPQYRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGS
  GSEAASLSSLNSSESDQDQDYDYLNEWGNRFKKLADMYGGGEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009864
• ORF Size: 2652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009864.3, NP_033994.1
• RefSeq Size: 4413 bp
• RefSeq ORF: 2655 bp
• Locus ID: 12550
• UniProt ID: P09803
• Cytogenetics: 8 53.18 cM
• MW: 98.7 kDa
• Gene Summary: This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015]