Ctsb (NM_007798) Mouse Tagged ORF Clone

CAT#: MR227342

Ctsb (Myc-DDK-tagged) - Mouse cathepsin B (Ctsb)

Product Images

Circular map for MR227342

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctsb
• Synonyms: CB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227342 representing NM_007798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGTCCTTGATCCTTCTTTCTTGCCTGCTGGCACTGACCAGTGCCCATGACAAGCCTTCCTTCC
  ACCCGCTGTCGGATGACCTGATTAACTATATCAACAAACAGAATACAACATGGCAGGCTGGACGCAACTT
  CTACAATGTTGACATAAGCTATCTGAAGAAGCTGTGTGGCACTGTCCTGGGTGGACCCAAACTGCCAGGA
  AGGGTTGCGTTCGGTGAGGACATAGATCTACCTGAAACCTTTGATGCACGGGAACAATGGTCCAACTGCC
  CGACCATTGGACAGATTAGAGACCAGGGCTCCTGCGGCTCTTGTTGGGCATTTGGGGCAGTGGAAGCCAT
  TTCTGACCGAACCTGCATTCACACCAATGGCCGAGTCAACGTGGAGGTGTCTGCTGAAGACCTGCTTACT
  TGCTGTGGTATCCAGTGTGGGGACGGCTGTAATGGTGGCTATCCCTCTGGAGCATGGAGCTTCTGGACAA
  AAAAAGGCCTGGTTTCAGGTGGAGTCTACAATTCTCATGTAGGCTGCTTACCATACACCATCCCTCCCTG
  CGAGCACCATGTCAATGGCTCCCGTCCCCCATGCACTGGAGAAGGAGATACTCCCAGGTGCAACAAGAGC
  TGTGAAGCTGGCTACTCCCCATCCTACAAAGAGGATAAGCACTTTGGGTACACTTCCTACAGCGTGTCTA
  ACAGTGTGAAGGAGATCATGGCAGAAATCTACAAAAATGGCCCAGTGGAGGGTGCCTTCACTGTGTTTTC
  TGACTTCTTGACTTACAAATCAGGAGTATACAAGCATGAAGCCGGTGATATGATGGGTGGCCACGCCATC
  CGCATCCTGGGCTGGGGAGTAGAGAATGGAGTTCCCTACTGGCTGGCAGCCAACTCTTGGAACCTTGACT
  GGGGTGATAATGGCTTCTTTAAAATCCTCAGAGGAGAGAACCACTGTGGCATTGAATCAGAAATTGTGGC
  TGGAATCCCACGCACTGACCAGTACTGGGGAAGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227342 representing NM_007798
  Red=Cloning site Green=Tags(s)
  
  MWWSLILLSCLLALTSAHDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPG
  RVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLT
  CCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKS
  CEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAI
  RILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007798
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007798.3, NP_031824.1
• RefSeq Size: 4756 bp
• RefSeq ORF: 1020 bp
• Locus ID: 13030
• UniProt ID: P10605
• Cytogenetics: 14 33.24 cM
• MW: 37.7 kDa
• Gene Summary: This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]