Erbb2 (NM_001003817) Mouse Tagged ORF Clone

CAT#: MR227307

  • TrueORF®

Erbb2 (Myc-DDK-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_001003817" in other vectors (4)


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Reconstitution Protocol

USD 1,640.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Rabbit polyclonal Phospho-Mouse ERBB2(Y1140) Antibody
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Erbb2
Synonyms c-erbB2; c-neu; Erbb-2; HER-2; HER2; l11Jus8; mKIAA3023; Neu
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR227307 representing NM_001003817
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGCGGCCTGGTGCCGTTGGGGGTTCCTCCTCGCCCTCCTGTCCCCCGGAGCCGCGGGTACCC
AAGTGTGTACCGGTACCGACATGAAGTTGCGACTCCCTGCCAGTCCTGAGACCCACCTGGACATGCTTCG
CCACCTCTACCAGGGCTGTCAGGTGGTGCAGGGCAATTTGGAGCTTACCTACCTGCCCGCCAATGCCAGC
CTCTCATTCCTGCAGGACATCCAGGAAGTCCAGGGATACATGCTCATCGCTCACAACCGAGTGAAACACG
TCCCACTGCAGAGGTTGCGCATCGTGAGAGGGACTCAGCTCTTTGAGGACAAGTATGCCCTGGCTGTGCT
AGACAACCGAGACCCTTTGGACAACGTCACCACCGCCGCCCCAGGCAGAACCCCAGAAGGGCTGCGGGAG
CTGCAGCTTCGAAGTCTCACAGAGATCTTGAAGGGAGGAGTTTTGATCCGTGGGAACCCTCAGCTCTGCT
ACCAGGACATGGTTTTGTGGAAGGATGTCCTCCGTAAGAATAACCAGCTGGCTCCTGTCGACATGGACAC
CAATCGTTCCCGGGCCTGTCCACCTTGTGCCCCAACCTGCAAAGACAATCACTGTTGGGGTGAGAGTCCT
GAAGACTGTCAGATCTTGACTGGCACCATCTGTACTAGTGGCTGTGCCCGGTGCAAGGGCCGGCTGCCCA
CTGACTGTTGCCATGAGCAGTGTGCTGCAGGCTGCACGGGTCCCAAGCATTCTGACTGCCTGGCCTGCCT
CCACTTCAATCATAGTGGTATCTGTGAGCTGCACTGCCCGGCCCTCATCACCTACAACACAGACACCTTC
GAGTCCATGCTCAACCCTGAGGGTCGCTACACCTTTGGTGCCAGCTGTGTGACCACCTGCCCCTACAACT
ACCTCTCCACGGAAGTGGGATCCTGCACTCTGGTCTGTCCCCCGAACAACCAAGAGGTCACAGCTGAGGA
CGGAACACAGCGGTGTGAGAAATGCAGCAAGCCCTGTGCTGGAGTATGCTATGGTCTGGGCATGGAGCAC
CTCCGAGGGGCGAGGGCCATCACCAGTGACAATATCCAGGAGTTTGCTGGCTGCAAGAAGATCTTTGGGA
GCCTGGCATTTTTGCCGGAGAGCTTTGATGGGAACCCCTCCTCCGGCGTTGCCCCACTGAAGCCAGAGCA
TCTCCAAGTGTTCGAAACCCTGGAGGAGATCACAGGTTACCTATACATTTCAGCATGGCCAGAGAGCTTC
CAAGACCTCAGTGTCTTCCAGAACCTTCGGGTCATTCGGGGACGGATTCTCCATGATGGTGCTTACTCAT
TGACGTTGCAAGGCCTGGGGATTCACTCACTGGGGCTACGCTCACTGCGGGAGCTGGGCAGTGGATTGGC
TCTCATTCACCGCAACACCCATCTCTGCTTTGTAAACACTGTACCTTGGGACCAGCTCTTCCGGAACCCG
CACCAGGCCCTACTCCACAGTGGGAACCGGCCAGAAGAGGCATGTGGTCTTGAGGGCTTGGTCTGTAACT
CACTGTGTGCCCGTGGGCACTGCTGGGGGCCAGGGCCCACCCAGTGTGTCAACTGCAGTCAGTTCCTCCG
GGGCCAGGAGTGTGTGGAGGAGTGCCGAGTATGGAAGGGGCTCCCCAGGGAGTATGTGAGGGGCAAGCAC
TGTCTGCCATGCCACCCCGAGTGTCAGCCTCAAAACAGCTCGGAGACCTGCTATGGATCGGAGGCTGACC
AGTGTGAGGCTTGTGCCCACTACAAGGACTCATCTTCCTGTGTGGCTCGCTGCCCCAGTGGTGTGAAGCC
AGACCTCTCCTACATGCCTATCTGGAAGTACCCGGATGAGGAGGGCATATGTCAGCCATGCCCCATCAAC
TGCACCCACTCATGTGTGGACCTGGACGAACGAGGCTGCCCAGCAGAGCAGAGAGCCAGCCCAGTGACAT
TCATCATTGCAACTGTGGTGGGCGTCCTGTTGTTCCTGATCATAGTGGTGGTCATTGGAATCCTAATCAA
ACGAAGGCGACAGAAGATCCGGAAGTATACCATGCGTAGGCTGCTGCAGGAGACCGAGCTGGTGGAGCCG
CTGACGCCCAGTGGAGCTGTGCCCAACCAGGCTCAGATGCGGATCCTAAAGGAGACAGAGCTAAGGAAGC
TGAAGGTGCTTGGGTCAGGAGCCTTCGGCACTGTCTACAAGGGCATCTGGATCCCAGATGGGGAGAACGT
GAAAATCCCCGTGGCCATCAAGGTGTTGAGGGAAAACACATCTCCTAAAGCTAACAAAGAAATCCTAGAT
GAAGCGTACGTCATGGCTGGTGTGGGTTCTCCATATGTGTCCCGCCTCCTGGGCATCTGCCTGACATCCA
CAGTGCAGCTGGTGACACAGCTTATGCCCTATGGCTGCCTTCTGGACCATGTCCGAGAACACCGAGGTCG
CTTAGGCTCCCAGGACCTGCTCAACTGGTGTGTTCAGATTGCCAAGGGGATGAGCTACCTGGAGGAAGTT
CGGCTTGTTCACAGGGACCTAGCTGCCCGAAACGTGCTAGTCAAGAGTCCCAACCACGTCAAGATTACCG
ACTTCGGGCTGGCACGGCTGCTGGACATTGATGAGACTGAATACCATGCAGATGGGGGCAAGGTGCCCAT
CAAGTGGATGGCATTGGAATCTATTCTCAGACGCCGGTTCACCCATCAGAGTGATGTGTGGAGCTATGGT
GTGACTGTGTGGGAGCTGATGACCTTTGGGGCCAAACCTTACGATGGGATCCCAGCTCGGGAGATCCCTG
ATTTGCTGGAGAAGGGAGAACGCCTACCTCAGCCTCCAATCTGCACCATCGACGTCTACATGATCATGGT
CAAATGTTGGATGATTGACTCCGAATGTCGCCCGAGATTCCGGGAGTTGGTATCAGAATTCTCCCGTATG
GCAAGGGACCCCCAGCGCTTTGTGGTCATCCAGAACGAGGACTTAGGCCCCTCCAGCCCCATGGACAGCA
CCTTCTACCGTTCACTGCTGGAGGATGATGACATGGGGGAGCTGGTCGATGCTGAAGAGTACCTGGTACC
CCAGCAGGGATTCTTCTCCCCAGACCCTGCCCTAGGTACTGGGAGCACAGCCCACCGCAGACACCGCAGC
TCGTCGGCCAGGAGTGGCGGTGGTGAGCTGACACTGGGCCTGGAGCCCTCGGAAGAAGAGCCCCCCAGAT
CTCCACTGGCTCCCTCCGAAGGGGCTGGCTCCGATGTGTTTGATGGTGACCTGGCAGTGGGGGTAACCAA
AGGACTGCAGAGCCTCTCTCCACATGACCTCAGCCCTCTACAGCGGTACAGTGAGGATCCCACATTACCT
CTGCCCCCCGAGACTGATGGCTACGTTGCTCCCCTGGCCTGCAGCCCCCAGCCCGAGTATGTGAACCAGC
CAGAGGTTCGGCCTCAGTCTCCCTTGACCCCAGAGGGTCCTCCGCCTCCCATCCGACCTGCTGGTGCTAC
TCTAGAAAGACCCAAGACTCTCTCTCCTGGGAAAAATGGGGTTGTCAAAGACGTTTTTGCCTTTGGGGGT
GCTGTGGAGAACCCTGAATACTTAGCACCCAGAGCAGGCACTGCCTCTCAGCCCCACCCTTCTCCTGCCT
TCAGCCCAGCCTTTGACAACCTCTATTACTGGGACCAGAACTCATCGGAGCAGGGTCCTCCACCAAGTAC
CTTTGAAGGGACCCCCACTGCAGAGAACCCTGAGTACCTAGGCCTGGATGTGCCAGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227307 representing NM_001003817
Red=Cloning site Green=Tags(s)

MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANAS
LSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRE
LQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESP
EDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTF
ESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEH
LRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESF
QDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNP
HQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKH
CLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPIN
CTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETELVEP
LTPSGAVPNQAQMRILKETELRKLKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILD
EAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVREHRGRLGSQDLLNWCVQIAKGMSYLEEV
RLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYG
VTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRM
ARDPQRFVVIQNEDLGPSSPMDSTFYRSLLEDDDMGELVDAEEYLVPQQGFFSPDPALGTGSTAHRRHRS
SSARSGGGELTLGLEPSEEEPPRSPLAPSEGAGSDVFDGDLAVGVTKGLQSLSPHDLSPLQRYSEDPTLP
LPPETDGYVAPLACSPQPEYVNQPEVRPQSPLTPEGPPPPIRPAGATLERPKTLSPGKNGVVKDVFAFGG
AVENPEYLAPRAGTASQPHPSPAFSPAFDNLYYWDQNSSEQGPPPSTFEGTPTAENPEYLGLDVPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001003817
ORF Size 3768 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001003817.1, NP_001003817.1
RefSeq Size 4998 bp
RefSeq ORF 3771 bp
Locus ID 13866
UniProt ID P70424
Cytogenetics 11 D
MW 139 kDa
Gene Summary Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).[UniProtKB/Swiss-Prot Function]

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