Tnfrsf13b (NM_021349) Mouse Tagged ORF Clone

CAT#: MR227278

Tnfrsf13b (Myc-DDK-tagged) - Mouse tumor necrosis factor receptor superfamily, member 13b (Tnfrsf13b)

Product Images

Circular map for MR227278

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnfrsf13b
• Synonyms: 1200009E08Rik; Taci
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227278 representing NM_021349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGGCATTCTGCCCCAAAGATCAGTACTGGGACTCCTCAAGGAAATCCTGTGTCTCCTGTGCAC
  TGACCTGCAGCCAGAGGAGCCAGCGCACCTGTACAGACTTCTGCAAATTCATCAATTGCCGAAAAGAGCA
  AGGCAGGTACTACGACCATCTCCTGGGGGCCTGCGTCAGCTGTGACTCCACCTGCACACAGCACCCTCAG
  CAGTGTGCCCACTTCTGTGAGAAAAGGCCCAGAAGCCAGGCGAACCTCCAGCCCGAGCTCGGGAGACCAC
  AGGCCGGGGAGGTGGAAGTCAGGTCAGACAACTCAGGAAGGCACCAGGGATCTGAGCATGGTCCAGGATT
  GAGGCTAAGTAGCGACCAGCTGACTCTCTACTGCACACTGGGGGTCTGCCTCTGCGCCATCTTCTGCTGT
  TTCTTGGTGGCCTTGGCCTCCTTCCTCAGGCGTAGAGGAGAGCCACTACCCAGCCAGCCTGCCGGGCCAC
  GTGGGTCACAAGCAAACTCTCCCCACGCCCACCGCCCCGTGACAGAGGCTTGCGACGAGGTGACCGCGTC
  ACCCCAGCCTGTGGAAACGTGTAGCTTCTGCTTCCCGGAGCGCAGTTCTCCCACTCAGGAGAGCGCGCCG
  CGTTCGCTCGGGATACACGGCTTCGCGGGCACTGCCGCCCCGCAGCCCTGTATGCGTGCAACAGTAGGCG
  GCCTGGGTGTCCTGCGCGCATCCACTGGGGACGCTCGTCCGGCAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227278 representing NM_021349
  Red=Cloning site Green=Tags(s)
  
  MAMAFCPKDQYWDSSRKSCVSCALTCSQRSQRTCTDFCKFINCRKEQGRYYDHLLGACVSCDSTCTQHPQ
  QCAHFCEKRPRSQANLQPELGRPQAGEVEVRSDNSGRHQGSEHGPGLRLSSDQLTLYCTLGVCLCAIFCC
  FLVALASFLRRRGEPLPSQPAGPRGSQANSPHAHRPVTEACDEVTASPQPVETCSFCFPERSSPTQESAP
  RSLGIHGFAGTAAPQPCMRATVGGLGVLRASTGDARPAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021349
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021349.3
• RefSeq Size: 750 bp
• RefSeq ORF: 750 bp
• Locus ID: 57916
• UniProt ID: Q9ET35
• Cytogenetics: 11 B2
• MW: 27.4 kDa
• Gene Summary: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity (By similarity).[UniProtKB/Swiss-Prot Function]