Stat3 (NM_213659) Mouse Tagged ORF Clone

CAT#: MR227265

Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1

Product Images

Circular map for MR227265

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stat3
• Synonyms: 1110034C02Rik; A; Aprf; AW109958
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227265 representing NM_213659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTGGAACCAGCTGCAGCAGCTGGACACACGCTACCTGGAGCAGCTGCACCAGCTGTACAGCG
  ACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGACTGGGCATATGCAGC
  CAGCAAAGAGTCACATGCCACGTTGGTGTTTCATAATCTCTTGGGTGAAATTGACCAGCAATATAGCCGA
  TTCCTGCAAGAGTCCAATGTCCTCTATCAGCACAACCTTCGAAGAATCAAGCAGTTTCTGCAGAGCAGGT
  ATCTTGAGAAGCCAATGGAAATTGCCCGGATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCA
  GACGGCAGCCACGGCAGCCCAGCAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAG
  CAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGG
  TGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGA
  TCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTG
  GACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGA
  CACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAA
  CATCTGCCTGGACCGTCTGGAAAACTGGATAACTTCATTAGCAGAATCTCAACTTCAGACCCGCCAACAA
  ATTAAGAAACTGGAGGAGCTGCAGCAGAAAGTGTCCTACAAGGGCGACCCTATCGTGCAGCACCGGCCCA
  TGCTGGAGGAGAGGATCGTGGAGCTGTTCAGAAACTTAATGAAGAGTGCCTTCGTGGTGGAGCGGCAGCC
  CTGCATGCCCATGCACCCGGACCGGCCCTTAGTCATCAAGACTGGTGTCCAGTTTACCACGAAAGTCAGG
  TTGCTGGTCAAATTTCCTGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGATAAAGACTCTGGGG
  ATGTTGCTGCCCTCAGAGGGTCTCGGAAATTTAACATTCTGGGCACGAACACAAAAGTGATGAACATGGA
  GGAGTCTAACAACGGCAGCCTGTCTGCAGAGTTCAAGCACCTGACCCTTAGGGAGCAGAGATGTGGGAAT
  GGAGGCCGTGCCAATTGTGATGCCTCCTTGATCGTGACTGAGGAGCTGCACCTGATCACCTTCGAGACTG
  AGGTGTACCACCAAGGCCTCAAGATTGACCTAGAGACCCACTCCTTGCCAGTTGTGGTGATCTCCAACAT
  CTGTCAGATGCCAAATGCTTGGGCATCAATCCTGTGGTATAACATGCTGACCAATAACCCCAAGAACGTG
  AACTTCTTCACTAAGCCGCCAATTGGAACCTGGGACCAAGTGGCCGAGGTGCTCAGCTGGCAGTTCTCGT
  CCACCACCAAGCGGGGGCTGAGCATCGAGCAGCTGACAACGCTGGCTGAGAAGCTCCTAGGGCCTGGTGT
  GAACTACTCAGGGTGTCAGATCACATGGGCTAAATTCTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCC
  TTCTGGGTCTGGCTAGACAATATCATCGACCTTGTGAAAAAGTATATCTTGGCCCTTTGGAATGAAGGGT
  ACATCATGGGTTTCATCAGCAAGGAGCGGGAGCGGGCCATCCTAAGCACAAAGCCCCCGGGCACCTTCCT
  ACTGCGCTTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGGAAAAGGACATCAGTGGC
  AAGACCCAGATCCAGTCTGTAGAGCCATACACCAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCA
  TCATGGGCTATAAGATCATGGATGCGACCAACATCCTGGTGTCTCCACTTGTCTACCTCTACCCCGACAT
  TCCCAAGGAGGAGGCATTTGGAAAGTACTGTAGGCCCGAGAGCCAGGAGCACCCCGAAGCCGACCCAGGT
  AGTGCTGCCCCGTACCTGAAGACCAAGTTCATCTGTGTGACACCAACGACCTGCAGCAATACCATTGACC
  TGCCGATGTCCCCCCGCACTTTAGATTCATTGATGCAGTTTGGAAATAACGGTGAAGGTGCTGAGCCCTC
  AGCAGGAGGGCAGTTTGAGTCGCTCACGTTTGACATGGATCTGACCTCGGAGTGTGCTACCTCCCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227265 representing NM_213659
  Red=Cloning site Green=Tags(s)
  
  MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSR
  FLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEK
  QQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTAL
  DQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQ
  IKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVR
  LLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGN
  GGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNV
  NFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFS
  FWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISG
  KTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
  SAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_213659
• ORF Size: 2310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213659.3
• RefSeq Size: 4487 bp
• RefSeq ORF: 2313 bp
• Locus ID: 20848
• UniProt ID: P42227
• Cytogenetics: 11 63.82 cM
• MW: 88.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]