Foxo1 (NM_019739) Mouse Tagged ORF Clone

CAT#: MR227240

Foxo1 (Myc-DDK-tagged) - Mouse forkhead box O1 (Foxo1)

Product Images

Circular map for MR227240

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxo1
• Synonyms: Afxh; AI876417; FKHR; Fkhr1; Foxo1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227240 representing NM_019739
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAGCGCCCCAGGTGGTGGAGACCGACCCGGACTTCGAGCCGCTGCCCCGGCAGCGCTCCTGTA
  CCTGGCCGCTGCCCAGGCCGGAGTTTAACCAGTCCAACTCGACCACCTCCAGTCCGGCGCCGTCGGGCGG
  CGCGGCCGCCAACCCCGACGCCGCGGCGAGCCTGGCCTCGGCGTCCGCTGTCAGCACCGACTTTATGAGC
  AACCTGAGCCTGCTGGAGGAGAGTGAGGACTTCGCGCGGGCGCCAGGCTGCGTGGCCGTGGCGGCGGCGG
  CTGCGGCCAGCAGGGGCCTGTGCGGGGACTTCCAGGGCCCCGAGGCGGGCTGCGTGCACCCAGCGCCGCC
  ACAGCCCCCACCGACCGGGCCGCTGTCGCAGCCCCCACCCGTGCCTCCCTCCGCTGCCGCCGCCGCGGGG
  CCACTCGCGGGACAGCCGCGCAAGACCAGCTCGTCGCGCCGCAACGCGTGGGGCAACCTGTCGTACGCCG
  ACCTCATCACCAAGGCCATCGAGAGCTCAGCCGAGAAGAGGCTCACCCTGTCGCAGATCTACGAGTGGAT
  GGTGAAGAGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCGGCGGGCTGGAAGAATTCAATT
  CGCCACAATCTGTCCCTTCACAGCAAGTTTATTCGAGTGCAGAATGAAGGAACTGGAAAGAGTTCTTGGT
  GGATGCTCAATCCAGAGGGAGGCAAGAGCGGAAAATCACCCCGGAGAAGAGCTGCGTCCATGGACAACAA
  CAGTAAATTTGCTAAGAGCCGAGGGCGGGCTGCTAAGAAAAAAGCATCTCTCCAGTCTGGGCAAGAGGGT
  CCTGGAGACAGCCCTGGGTCTCAGTTTTCTAAGTGGCCTGCGAGTCCTGGGTCCCACAGCAACGATGACT
  TTGATAACTGGAGTACATTTCGTCCTCGAACCAGCTCAAATGCTAGTACCATCAGTGGGAGACTTTCTCC
  CATCATGACAGAGCAGGATGACCTGGGAGATGGGGACGTGCATTCCCTGGTGTATCCACCCTCTGCTGCC
  AAGATGGCGTCTACGCTGCCCAGTCTGTCTGAAATCAGCAATCCAGAAAACATGGAGAACCTTCTGGATA
  ATCTCAACCTTCTCTCGTCCCCAACATCTTTAACTGTGTCCACCCAGTCCTCGCCTGGCAGCATGATGCA
  GCAGACACCATGCTATTCGTTTGCACCGCCAAACACCAGTCTAAATTCACCCAGTCCAAACTACTCAAAG
  TACACATACGGCCAATCCAGCATGAGCCCTTTGCCCCAGATGCCTATGCAGACACTTCAGGACAGCAAAT
  CAAGTTACGGAGGATTGAACCAGTATAACTGTGCCCCAGGACTCTTGAAAGAGTTGTTGACTTCTGACTC
  TCCTCCCCACAATGACATTATGTCACCGGTTGATCCCGGAGTGGCCCAACCCAACAGTCGGGTCCTGGGC
  CAAAATGTAATGATGGGCCCTAATTCGGTCATGCCAGCGTATGGCAGCCAGGCATCTCATAACAAAATGA
  TGAACCCCAGCTCCCACACCCACCCTGGACATGCACAGCAAACGGCTTCGGTCAACGGCCGTACCCTGCC
  CCATGTGGTGAACACCATGCCTCACACATCTGCCATGAACCGCTTGACCCCCGTGAAGACACCTTTACAA
  GTGCCTCTGTCCCACCCCATGCAGATGAGTGCCCTGGGCAGCTACTCCTCGGTGAGCAGCTGCAATGGCT
  ATGGTAGGATGGGTGTCCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGGCATGTTTATTGAGCGCTT
  GGACTGTGACATGGAGTCCATCATTCGGAATGACCTCATGGATGGAGATACCTTGGATTTTAACTTTGAT
  AATGTGTTGCCCAACCAAAGCTTCCCACACAGTGTCAAGACTACAACACACAGCTGGGTGTCAGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227240 representing NM_019739
  Red=Cloning site Green=Tags(s)
  
  MAEAPQVVETDPDFEPLPRQRSCTWPLPRPEFNQSNSTTSSPAPSGGAAANPDAAASLASASAVSTDFMS
  NLSLLEESEDFARAPGCVAVAAAAAASRGLCGDFQGPEAGCVHPAPPQPPPTGPLSQPPPVPPSAAAAAG
  PLAGQPRKTSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSI
  RHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEG
  PGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSLVYPPSAA
  KMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGSMMQQTPCYSFAPPNTSLNSPSPNYSK
  YTYGQSSMSPLPQMPMQTLQDSKSSYGGLNQYNCAPGLLKELLTSDSPPHNDIMSPVDPGVAQPNSRVLG
  QNVMMGPNSVMPAYGSQASHNKMMNPSSHTHPGHAQQTASVNGRTLPHVVNTMPHTSAMNRLTPVKTPLQ
  VPLSHPMQMSALGSYSSVSSCNGYGRMGVLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFD
  NVLPNQSFPHSVKTTTHSWVSG
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019739
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019739.3, NP_062713.2
• RefSeq Size: 5552 bp
• RefSeq ORF: 1959 bp
• Locus ID: 56458
• UniProt ID: Q9R1E0
• Cytogenetics: 3 23.19 cM
• MW: 70 kDa
• Gene Summary: Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).[UniProtKB/Swiss-Prot Function]