Cyld (NM_173369) Mouse Tagged ORF Clone

CAT#: MR227170

Cyld (Myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 2

Product Images

Circular map for MR227170

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyld
• Synonyms: 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227170 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGATTTTTTATCTGC
  TTCTTCAAGAATGCAGTGTAACAGACAAACAAACTCAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGG
  ACAGTACATCCAAGACCGTTCTGTGGGGCATTCAAGAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATT
  GGATTAAAAATCTTGGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG
  AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACT
  CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAG
  AAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG
  GAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGTTTCACGGATGGGGTATATCAAGGGAAGCAGCTTTT
  CCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCATTGGACAAACTAGAACTTATAGAAGATGATGACAAT
  GGATTGGAAAGTGATTTTGCAGGCCCAGGAGATACAATGCAGGTTGAACCTCCCCCTTTGGAAATAAACT
  CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTAATATTCTGTGATGTTTTACCAGG
  AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGGTTT
  GATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGAAAGTACAATTCTCCTGCACATCAATGACATCATCC
  CAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGG
  TTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAACAGATCTGAA
  TTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAATCCAAGTCCAAAAATCCATGGTACATTG
  ATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACAGAGATGTCTTCGGACTTCGGACATTCATCTCCTCC
  ACCGCAGCCTCCTTCCATGAACTCCTTGTCTAGCGAGAACAGATTCCACTCCTTACCCTTCAGCCTGACA
  AAGATGCCCAATACTAATGGCAGCATGGCTCATAGTCCACTCTCTCTGTCAGTGCAGTCTGTGATGGGGG
  AGCTGAACAGCACACCTGTCCAGGAGAGTCCACCCTTGCCCATCTCTTCTGGGAATGCACACGGGCTAGA
  GGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCCCGTTCTATGGGGTTATCCGTTGGATTGGCCAGCCA
  CCAGGGCTCAGTGACGTGCTAGCTGGACTGGAACTGGAAGATGAATGCGCAGGCTGTACAGATGGAACTT
  TCAGGGGCACGCGGTATTTCACGTGTGCCCTGAAGAAGGCACTGTTTGTGAAACTGAAGAGCTGCAGACC
  GGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCAATCAAATTGAAAGGTGTAACTCTTTAGCATTTGGG
  GGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCCACCTAAAATGGAAAAGGAAGGTTTAGAGATAATGA
  TTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACTTTATTCTGCTTATT
  TGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTAGACCCAAAGAGAAGAATGATATAGAGTATTACAGT
  GAGACTCAGGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATATGTGTGTGCCACAA
  AGATTATGAAACTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGTTTTACCTCTGAAGAAAAAGA
  TCCTGAAGAATTCTTAAATATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGTTAAAAATAAGATCA
  GCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGAGAAAGTTGGAGTAC
  CCACAATTCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCAGAGGCACCATCATG
  CTTGATTATCCAGATGCCTCGGTTTGGAAAAGACTTTAAACTATTTAAAAAAATTTTTCCTTCCCTGGAA
  TTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGGACTTGCGATGTACG
  AGTGCAGGGAGTGCTATGACGATCCGGACATCTCAGCTGGGAAGATCAAGCAGTTCTGTAAGACCTGCAG
  CACTCAGGTTCACCTTCATCCCAGGAGGTTGAATCATTCTTATCATCCAGTATCACTTCCCAAAGACTTG
  CCTGACTGGGACTGGAGACACGGCTGCATCCCCTGTCAGAAGATGGAGTTATTTGCTGTTCTCTGCATAG
  AAACGAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGATGACTCTGCCTGGCTTTTCTTTGACAGCAT
  GGCGGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACGCCCTGCCCAGAAGTGGGAGAG
  TACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGATTCCAGAAGGATTCAAGGCTGTGCGCGCAGAC
  TTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCCTGTACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227170 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRVPSTKGKKNQI
  GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRLRLSKGLQVDVGSPVKVQLRSGEE
  KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN
  GLESDFAGPGDTMQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF
  DGVQLCSFASVESTILLHINDIIPDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRNRSE
  LFYTLNGSSVDSQQSKSKNPWYIDEVAEDPAKSLTEMSSDFGHSSPPPQPPSMNSLSSENRFHSLPFSLT
  KMPNTNGSMAHSPLSLSVQSVMGELNSTPVQESPPLPISSGNAHGLEVGSLAEVKENPPFYGVIRWIGQP
  PGLSDVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFG
  GYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKNDIEYYS
  ETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDILRVEPLLKIRS
  AGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLE
  LNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCSTQVHLHPRRLNHSYHPVSLPKDL
  PDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGE
  YLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173369
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173369.3
• RefSeq Size: 8014 bp
• RefSeq ORF: 2859 bp
• Locus ID: 74256
• UniProt ID: Q80TQ2
• Cytogenetics: 8 C3
• MW: 106.6 kDa
• Gene Summary: This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]