Agmo (NM_178767) Mouse Tagged ORF Clone

CAT#: MR227089

Agmo (Myc-DDK-tagged) - Mouse alkylglycerol monooxygenase (Agmo)

Product Images

Circular map for MR227089

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agmo
• Synonyms: A530016O06Rik; AI790538; Tmem195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227089 representing NM_178767
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGCCCAGGAGCCCAAGACAATGTTTCAGTTTCTCAGGGTATGCGAGCGATGTTTTACATGATGG
  AGCCCAGTGAAACTGCCTTCCAAACTGTTGAAGAGGTGCCTGATTACGTAAAAAAGGCGACTCCATTTTT
  TATCTTCTTGATTCTGCTTGAACTAGTCATCAGCTGGATTCTCAAAGGGAAACCATCAGGTCGTTTGGAT
  GATGCTCTCACATCTATATCAGCTGGTGTTGTGTCTCGGCTTCCAAGTCTGTTCTTCAGGAGCCTTGAAG
  TGACCAGTTATATTTATATCTGGGAAAACTACAGACTTTTGGAATTGCCTTGGGATTCTACCTGGACGTG
  GTATTTCACTTTCTTAGGAGTTGACTTTGGCTACTACTGGTTCCACCGCATGGCTCATGAGATTAATATT
  TTCTGGGCAGCACACCAAGCACACCACAGTTCTGAAGACTATAACTTATCCACAGCACTGAGACAGTCTG
  TCTTGCAGCAGTATAGTTCCTGGGTTTTCTACTGTCCACTGGCCCTTTTCATACCACCTTCAGTGTTTGC
  TGTTCATATTCAATTCAATCTCCTCTACCAATTTTGGATCCACACAGAGATCATCAGAACCCTTGGCCCT
  TTGGAAGTGATCCTTAATACTCCTAGCCATCACAGAGTTCATCATGGTAGAAATCGTTACTGCATTGATA
  AAAACTATGCTGGCACTCTTATTATTTGGGATAGAATTTTTGGGACATTTGAAGCAGAGAATGAGCAGGT
  CATATATGGTCTAACCCATCCCATTGGTACATTTGAGCCATTCAACGTGCAGTTTCACCATTTACTTTAT
  ATATGGACTACATTCTGGACCACACCTGGATTCTGCCATAAGTTTTCTGTCCTATTTAAAGGACCAGGAT
  GGGGTCCAGGTAAACCAAGACTTGGCCTGAGTGAAGAAATTCCAGAGGTTACAGGACAGGAAGTCCCCTT
  CTCATCCTCAGCATCCCAGCTTTTAAAGATCTACACTGTCCTCCAGTTTGCAGTAATGCTGGCATTTTAT
  GAAGAGACCTTTGCAAACACAGCGGTACTGTCCCAGGTCACTCTTCTTCTGAGAATCTTCTTCTTTATCC
  TGACCTTGACGTCCATTGGTTTTCTTCTGGACCAAAGATCCAAGGCAGCTACTATGGAAACTTTCCGGTG
  CCTGTTGTTCCTAACCCTGCACAGATTTGGTCACTTAAAGCCTCTTATTCCATCTTTATCTTTTGCATTT
  GAGATTTTCTTTTCGGTTTGCATTGCTTTCTGGGGCGTAAGAAGCATAACACAACTCACCTCTGGCTCTT
  GGAAAAAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227089 representing NM_178767
  Red=Cloning site Green=Tags(s)
  
  MRSPGAQDNVSVSQGMRAMFYMMEPSETAFQTVEEVPDYVKKATPFFIFLILLELVISWILKGKPSGRLD
  DALTSISAGVVSRLPSLFFRSLEVTSYIYIWENYRLLELPWDSTWTWYFTFLGVDFGYYWFHRMAHEINI
  FWAAHQAHHSSEDYNLSTALRQSVLQQYSSWVFYCPLALFIPPSVFAVHIQFNLLYQFWIHTEIIRTLGP
  LEVILNTPSHHRVHHGRNRYCIDKNYAGTLIIWDRIFGTFEAENEQVIYGLTHPIGTFEPFNVQFHHLLY
  IWTTFWTTPGFCHKFSVLFKGPGWGPGKPRLGLSEEIPEVTGQEVPFSSSASQLLKIYTVLQFAVMLAFY
  EETFANTAVLSQVTLLLRIFFFILTLTSIGFLLDQRSKAATMETFRCLLFLTLHRFGHLKPLIPSLSFAF
  EIFFSVCIAFWGVRSITQLTSGSWKKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178767
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178767.5, NP_848882.2
• RefSeq Size: 2155 bp
• RefSeq ORF: 1344 bp
• Locus ID: 319660
• UniProt ID: Q8BS35
• Cytogenetics: 12 A3
• MW: 51.8 kDa
• Gene Summary: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes (By similarity).[UniProtKB/Swiss-Prot Function]