Trp73 (NM_011642) Mouse Tagged ORF Clone

CAT#: MR227042

Trp73 (Myc-DDK-tagged) - Mouse transformation related protein 73 (Trp73), transcript variant 1

Product Images

Circular map for MR227042

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trp73
• Synonyms: delta; deltaNp73; p7; p73; TAp; TAp73; Tp73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227042 representing NM_011642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCAGCGTTGGGGAGATGGCCCAGACCTCTTCTTCCTCCTCCTCCACCTTCGAGCACCTGTGGA
  GTTCTCTAGAGCCAGACAGCACCTACTTTGACCTCCCCCAGCCCAGCCAAGGGACTAGCGAGGCATCAGG
  CAGCGAGGAGTCCAACATGGATGTCTTCCACCTGCAAGGCATGGCCCAGTTCAATTTGCTCAGCAGTGCC
  ATGGACCAGATGGGCAGCCGTGCGGCCCCGGCGAGCCCCTACACCCCGGAGCACGCCGCCAGCGCGCCCA
  CCCACTCGCCCTACGCGCAGCCCAGCTCCACCTTCGACACCATGTCTCCGGCGCCTGTCATCCCTTCCAA
  TACCGACTACCCCGGCCCCCACCACTTCGAGGTCACCTTCCAGCAGTCGAGCACTGCCAAGTCGGCCACC
  TGGACATACTCCCCACTCTTGAAGAAGTTGTACTGTCAGATTGCTAAGACATGCCCCATCCAGATCAAAG
  TGTCCACACCACCACCCCCGGGCACGGCCATCCGGGCCATGCCTGTCTACAAGAAGGCAGAGCATGTGAC
  CGACATTGTTAAGCGCTGCCCCAACCACGAGCTTGGAAGGGACTTCAATGAAGGACAGTCTGCCCCGGCT
  AGCCACCTCATCCGTGTAGAAGGCAACAACCTCGCCCAGTACGTGGATGACCCTGTCACCGGAAGGCAGA
  GTGTGGTTGTGCCGTATGAACCCCCACAGGTGGGAACAGAATTTACCACCATCCTGTACAACTTCATGTG
  TAACAGCAGCTGTGTGGGGGGCATGAATCGGAGGCCCATCCTTGTCATCATCACCCTGGAGACCCGGGAT
  GGACAGGTCCTGGGCCGCCGGTCTTTCGAGGGTCGCATCTGTGCCTGTCCTGGCCGTGACCGCAAAGCTG
  ATGAAGACCATTACCGGGAGCAACAGGCTCTGAATGAAAGTACCACCAAAAATGGAGCTGCCAGCAAACG
  TGCATTCAAGCAGAGCCCCCCTGCCATCCCTGCCCTGGGTACCAACGTGAAGAAGAGACGCCACGGGGAC
  GAGGACATGTTCTACATGCACGTGCGAGGCCGGGAGAACTTTGAGATCTTGATGAAAGTCAAGGAGAGCC
  TAGAACTGATGGAGCTTGTGCCCCAGCCTTTGGTTGACTCCTATCGACAGCAGCAGCAGCAGCAGCTCCT
  ACAGAGGCCGAGTCACCTGCAGCCTCCATCCTATGGGCCCGTGCTCTCCCCAATGAACAAGGTACACGGT
  GGTGTCAACAAACTGCCCTCCGTCAACCAGCTGGTGGGCCAGCCTCCCCCGCACAGCTCAGCAGCTGGGC
  CCAACCTGGGGCCCATGGGCTCCGGGATGCTCAACAGCCACGGCCACAGCATGCCGGCCAATGGTGAGAT
  GAATGGAGGCCACAGCTCCCAGACCATGGTTTCGGGATCCCACTGCACCCCGCCACCCCCCTATCATGCA
  GACCCCAGCCTCGTCAGTTTTTTGACAGGGTTGGGGTGTCCAAACTGCATCGAGTGCTTCACTTCCCAAG
  GGTTGCAGAGCATCTACCACCTGCAGAACCTTACCATCGAGGACCTTGGGGCTCTGAAGGTCCCTGACCA
  GTACCGTATGACCATCTGGAGGGGCCTACAGGACCTGAAGCAGAGCCATGACTGCGGCCAGCAACTGCTA
  CGCTCCAGCAGCAACGCGGCCACCATCTCCATCGGCGGCTCTGGCGAGCTGCAGCGGCAGCGGGTCATGG
  AAGCCGTGCATTTCCGTGTGCGCCACACCATCACGATCCCCAACCGTGGAGGCGCAGGTGCGGTGACAGG
  TCCCGACGAGTGGGCGGACTTTGGCTTTGACCTGCCTGACTGCAAGTCCCGTAAGCAGCCCATCAAAGAG
  GAGTTCACAGAGACAGAGAGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227042 representing NM_011642
  Red=Cloning site Green=Tags(s)
  
  MSGSVGEMAQTSSSSSSTFEHLWSSLEPDSTYFDLPQPSQGTSEASGSEESNMDVFHLQGMAQFNLLSSA
  MDQMGSRAAPASPYTPEHAASAPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSAT
  WTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPA
  SHLIRVEGNNLAQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILVIITLETRD
  GQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESTTKNGAASKRAFKQSPPAIPALGTNVKKRRHGD
  EDMFYMHVRGRENFEILMKVKESLELMELVPQPLVDSYRQQQQQQLLQRPSHLQPPSYGPVLSPMNKVHG
  GVNKLPSVNQLVGQPPPHSSAAGPNLGPMGSGMLNSHGHSMPANGEMNGGHSSQTMVSGSHCTPPPPYHA
  DPSLVSFLTGLGCPNCIECFTSQGLQSIYHLQNLTIEDLGALKVPDQYRMTIWRGLQDLKQSHDCGQQLL
  RSSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGAGAVTGPDEWADFGFDLPDCKSRKQPIKE
  EFTETESH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011642
• ORF Size: 1914 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011642.3, NP_035772.2
• RefSeq Size: 5046 bp
• RefSeq ORF: 1896 bp
• Locus ID: 22062
• UniProt ID: Q9JJP2
• Cytogenetics: 4 83.79 cM
• MW: 70.2 kDa
• Gene Summary: This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq, Jul 2008]