Fgfr3 (NM_008010) Mouse Tagged ORF Clone

CAT#: MR226913

Fgfr3 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 1

Product Images

Circular map for MR226913

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr3
• Synonyms: CD333; Fgfr-; Fgfr-3; Flg-2; FR3; HBGF; HBGFR; Mfr3; sa; sam3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226913 representing NM_008010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTCCCGGCCTGCGTGCTAGTGTTCTGCGTGGCGGTCGTGGCTGGAGCTACTTCCGAGCCTCCTG
  GTCCAGAGCAGCGAGTTGTGCGGAGAGCGGCAGAGGTTCCAGGGCCTGAACCTAGCCAGCAGGAGCAGGT
  GGCCTTCGGCAGTGGGGACACCGTGGAGCTGAGCTGCCATCCTCCTGGAGGTGCCCCCACAGGGCCCACG
  GTCTGGGCTAAGGATGGTACAGGTCTGGTGGCCTCCCACCGCATCCTGGTGGGGCCTCAGAGGCTGCAAG
  TGCTAAATGCCTCCCACGAAGATGCAGGGGTCTACAGCTGCCAGCACCGGCTCACTCGGCGTGTGCTGTG
  CCACTTCAGTGTGCGTGTAACAGATGCTCCATCCTCAGGAGATGACGAAGATGGGGAGGACGTGGCTGAA
  GACACAGGGGCTCCTTATTGGACTCGCCCGGAGCGAATGGATAAGAAACTGCTGGCTGTGCCAGCCGCAA
  ACACTGTCCGCTTCCGCTGCCCAGCTGCTGGCAACCCTACCCCCTCCATCTCCTGGCTGAAGAATGGCAA
  AGAATTCCGAGGGGAGCATCGCATTGGGGGCATCAAGCTCCGGCACCAGCAGTGGAGCTTGGTCATGGAA
  AGTGTGGTACCCTCCGATCGTGGCAACTATACCTGTGTAGTTGAGAACAAGTTTGGCAGCATCCGGCAGA
  CATACACACTGGATGTGCTGGAGCGCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCA
  GACAGCCATTCTAGGCAGTGACGTGGAGTTCCACTGCAAGGTGTACAGCGATGCACAGCCACACATCCAG
  TGGCTGAAGCACGTGGAAGTGAACGGCAGCAAGGTGGGCCCTGACGGCACGCCCTACGTCACTGTACTCA
  AGACTGCAGGCGCTAACACCACCGACAAGGAGCTAGAGGTTCTGTCCTTGCACAATGTCACCTTTGAGGA
  CGCGGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGTTTTCCCATCACTCTGCGTGGCTGGTGGTG
  CTGCCAGCTGAGGAGGAGCTGATGGAAACTGATGAGGCTGGCAGCGTGTACGCAGGCGTCCTCAGCTACG
  GGGTGGTCTTCTTCCTCTTCATCCTGGTGGTGGCAGCTGTGATACTCTGCCGCCTGCGCAGTCCCCCAAA
  GAAGGGCTTGGGCTCGCCCACCGTGCACAAGGTCTCTCGCTTCCCGCTTAAGCGACAGGTGTCCTTGGAA
  TCTAACTCCTCTATGAACTCCAACACACCCCTTGTCCGGATTGCCCGGCTGTCCTCAGGAGAAGGTCCTG
  TTCTGGCCAATGTTTCTGAACTTGAGCTGCCTGCTGACCCCAAGTGGGAGCTATCCAGGACCCGGCTGAC
  ACTTGGTAAGCCTCTTGGAGAAGGCTGCTTTGGACAGGTGGTCATGGCAGAAGCTATTGGCATCGACAAG
  GACCGTACTGCCAAGCCTGTCACCGTGGCCGTGAAGATGCTGAAAGATGATGCGACTGACAAGGACCTGT
  CGGACCTGGTATCTGAGATGGAGATGATGAAAATGATTGGCAAGCACAAGAACATCATTAACCTGCTGGG
  GGCGTGCACACAGGGTGGGCCCCTGTATGTGCTGGTGGAGTACGCAGCCAAGGGCAATCTCCGGGAGTTC
  CTTCGGGCGCGGCGGCCTCCAGGCATGGACTACTCCTTTGATGCCTGCAGGCTGCCAGAGGAACAGCTCA
  CCTGCAAGGATCTAGTGTCCTGTGCCTACCAGGTGGCACGGGGCATGGAATACTTGGCTTCTCAGAAGTG
  TATTCACAGAGACTTGGCTGCCAGAAACGTCCTGGTGACCGAGGACAATGTGATGAAGATTGCGGACTTT
  GGCCTGGCTCGAGATGTGCACAACCTGGACTACTACAAGAAGACCACAAATGGCCGGCTACCTGTGAAGT
  GGATGGCACCAGAGGCCCTTTTTGACCGAGTCTACACCCACCAGAGTGATGTTTGGTCTTTTGGTGTCCT
  CCTCTGGGAGATCTTTACGCTGGGGGGCTCACCGTATCCTGGCATCCCAGTGGAAGAGCTTTTCAAGCTG
  TTGAAAGAGGGCCACCGCATGGACAAGCCAGCCAGCTGCACACATGACCTGTACATGATCATGCGGGAAT
  GTTGGCATGCGGTGCCTTCACAGAGGCCCACCTTCAAGCAGTTGGTAGAGGATTTAGACCGCATCCTCAC
  TGTGACATCAACCGACGAGTACTTGGACCTCTCCGTGCCGTTTGAGCAGTACTCGCCAGGTGGCCAGGAC
  ACGCCTAGCTCCAGCTCGTCCGGAGATGACTCGGTGTTCACCCATGACCTGCTACCCCCAGGTCCACCCA
  GTAACGGGGGACCTCGGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226913 representing NM_008010
  Red=Cloning site Green=Tags(s)
  
  MVVPACVLVFCVAVVAGATSEPPGPEQRVVRRAAEVPGPEPSQQEQVAFGSGDTVELSCHPPGGAPTGPT
  VWAKDGTGLVASHRILVGPQRLQVLNASHEDAGVYSCQHRLTRRVLCHFSVRVTDAPSSGDDEDGEDVAE
  DTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGKEFRGEHRIGGIKLRHQQWSLVME
  SVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAILGSDVEFHCKVYSDAQPHIQ
  WLKHVEVNGSKVGPDGTPYVTVLKTAGANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIGFSHHSAWLVV
  LPAEEELMETDEAGSVYAGVLSYGVVFFLFILVVAAVILCRLRSPPKKGLGSPTVHKVSRFPLKRQVSLE
  SNSSMNSNTPLVRIARLSSGEGPVLANVSELELPADPKWELSRTRLTLGKPLGEGCFGQVVMAEAIGIDK
  DRTAKPVTVAVKMLKDDATDKDLSDLVSEMEMMKMIGKHKNIINLLGACTQGGPLYVLVEYAAKGNLREF
  LRARRPPGMDYSFDACRLPEEQLTCKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTEDNVMKIADF
  GLARDVHNLDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLLWEIFTLGGSPYPGIPVEELFKL
  LKEGHRMDKPASCTHDLYMIMRECWHAVPSQRPTFKQLVEDLDRILTVTSTDEYLDLSVPFEQYSPGGQD
  TPSSSSSGDDSVFTHDLLPPGPPSNGGPRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008010
• ORF Size: 2400 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008010.5, NP_032036.2
• RefSeq Size: 4219 bp
• RefSeq ORF: 2403 bp
• Locus ID: 14184
• Cytogenetics: 5 17.83 cM
• MW: 88.1 kDa
• Gene Summary: This gene encodes a member of the fibroblast growth factor receptor family. Members of this family are highly conserved proteins that differ from one another in their ligand affinities and tissue distribution. A representative protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene may be associated with craniosynostosis and multiple types of skeletal dysplasia. A pseudogene of this gene is located on chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]