Tgfa (NM_031199) Mouse Tagged ORF Clone
CAT#: MR226910
- TrueORF®
Tgfa (Myc-DDK-tagged) - Mouse transforming growth factor alpha (Tgfa)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_031199" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tgfa |
Synonyms | wa-1; wa1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226910 representing NM_031199
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCGCGACCGGACAGCTCGCTCTGCTAGCGCTGGGTATCCTGTTAGCTGTGTGCCAGGCTCTGG AGAACAGCACATCCCCCCTGAGTGACTCACCCGTGGCGGCTGCAGTGGTGTCTCACTTCAACAAGTGCCC AGATTCCCACACTCAGTACTGCTTCCATGGAACCTGCCGGTTTTTGGTGCAGGAAGAGAAGCCAGCATGT GTCTGCCACTCTGGGTACGTGGGTGTTCGCTGTGAGCATGCAGACCTCCTGGCTGTGGTGGCTGCCAGCC AGAAGAAGCAAGCCATCACTGCCCTGGTGGTGGTCTCCATTGTGGCCCTGGCTGTCCTCATTATCACCTG TGTGCTGATCCACTGCTGTCAGCTCCGCAAACACTGTGAGTGGTGCCGTGCCCTCGTCTGCAGACATGAG AAGCCCAGCGCCCTCCTGAAGGGAAGGACTGCTTGCTGCCACTCTGAGACAGTGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226910 representing NM_031199
Red=Cloning site Green=Tags(s) MVPATGQLALLALGILLAVCQALENSTSPLSDSPVAAAVVSHFNKCPDSHTQYCFHGTCRFLVQEEKPAC VCHSGYVGVRCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQLRKHCEWCRALVCRHE KPSALLKGRTACCHSETVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_031199 |
ORF Size | 477 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031199.5 |
RefSeq Size | 4229 bp |
RefSeq ORF | 480 bp |
Locus ID | 21802 |
UniProt ID | P48030 |
Cytogenetics | 6 37.62 cM |
MW | 17.5 kDa |
Gene Summary | This gene encodes a member of the epidermal growth factor (EGF) family of proteins that regulate cellular proliferation. The encoded protein undergoes proteolytic processing to generate a soluble glycoprotein that is secreted by the cell. The secreted protein binds to the EGF receptors to initiate signaling events resulting in cellular proliferation, mucous production or inhibition of gastric acid secretion. The transgenic expression of the encoded protein in mice induces the development of cancers in various tissues such as liver, pancreas, skin and mammary glands. Mice lacking the encoded protein exhibit a wavy coat and curly whiskers phenotype as well as abnormalities in the eye. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203373 | Tgfa (untagged) - Mouse transforming growth factor alpha (Tgfa), (10ug) |
USD 150.00 |
|
MG226910 | Tgfa (tGFP-tagged) - Mouse transforming growth factor alpha (Tgfa), (10ug) |
USD 350.00 |
|
MR226910L3 | Lenti ORF clone of Tgfa (Myc-DDK-tagged) - Mouse transforming growth factor alpha (Tgfa) |
USD 450.00 |
|
MR226910L4 | Lenti ORF clone of Tgfa (mGFP-tagged) - Mouse transforming growth factor alpha (Tgfa) |
USD 450.00 |
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