Epo (NM_007942) Mouse Tagged ORF Clone
Epo (Myc-DDK-tagged) - Mouse erythropoietin (Epo)
|Product Name||Epo (NM_007942) Mouse Tagged ORF Clone|
|Type||Mouse Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>MR226885 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>MR226885 protein sequence
Red=Cloning site Green=Tags(s)
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||579 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||715 bp|
|RefSeq ORF||579 bp|
|Cytogenetics||5 76.5 cM|
|Gene Summary||This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]|
|cDNA Clone Resources|
|MC208445||Epo (untagged) - Mouse erythropoietin (Epo), (10ug)||
|MG226885||Epo (GFP-tagged) - Mouse erythropoietin (Epo), (10ug)||
|MR226885L1||Lenti ORF clone of Epo (Myc-DDK-tagged) - Mouse erythropoietin (Epo)||
|MR226885L2||Lenti ORF clone of Epo (mGFP-tagged) - Mouse erythropoietin (Epo)||
|MR226885L3||Lenti ORF clone of Epo (Myc-DDK-tagged) - Mouse erythropoietin (Epo)||
|MR226885L4||Lenti ORF clone of Epo (mGFP-tagged) - Mouse erythropoietin (Epo)||