Itgav (NM_008402) Mouse Tagged ORF Clone

CAT#: MR226884

Itgav (Myc-DDK-tagged) - Mouse integrin alpha V (Itgav)

Product Images

Circular map for MR226884

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgav
• Synonyms: 1110004F14Rik; 2610028E01Rik; CD51; D430040G12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226884 representing NM_008402
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCCCGGGCGCCTGCTGCTACGCCCTCGCCCCGGCGGCCTCCTGCTGCTGCTGCCGGGTCTCC
  TGCTGCCCCTCGCCGACGCCTTCAACCTGGACGTCGAAAGTCCCGCCGAGTATGCGGGTCCCGAGGGAAG
  TTACTTCGGATTCGCCGTGGACTTCTTCGAGCCCAGCACGTCCTCCAGGATGTTTCTCCTGGTGGGAGCC
  CCCAAAGCGAACACGACCCAGCCTGGGATTGTAGAAGGAGGGCAAGTTCTCAAATGTGAATGCTCATCCA
  GCCGCCGGTGCCAGCCCATTGAGTTTGATTCAACAGGCAATCGAGATTATGCCAAAGATGACCCACTGGA
  GTTTAAGTCCCACCAGTGGTTTGGAGCCTCTGTGAGGTCAAAGCAGGATAAAATCTTGGCCTGTGCTCCA
  TTGTACCACTGGAGGACTGAGATGAAGCAGGAGAGAGAGCCAGTTGGAACCTGCTTTCTTCAGGACGGCA
  CAAAGACCGTTGAGTATGCTCCATGCAGGTCAAAAAATATTGATGCTGATGGCCAGGGATTTTGTCAAGG
  AGGATTCAGCATTGATTTTACTAAAGCTGACAGAGTACTTCTCGGTGGTCCTGGTAGCTTTTATTGGCAA
  GGTCAGCTCATTTCGGACCAAGTGGCAGAAATCATATCTAAATATGACCCAAATGTCTACAGCATCAAAT
  ATAATAACCAATTAGCAACACGGACTGCACAAGCAATTTTTGATGACAGTTATTTGGGTTACTCTGTGGC
  CGTGGGAGACTTCAATGGTGACGGCATTGAAGATTTTGTCTCAGGAGTTCCAAGAGCAGCAAGGACTTTG
  GGAATGGTTTATATTTATGATGGGAAAAATATGTCCTCTTTACACAATTTTACTGGTGAACAGATGGCTG
  CGTATTTTGGATTTTCTGTAGCTGCTACTGACATTAATGGGGATGATTACGCAGATGTGTTTATTGGAGC
  CCCCCTGTTCATGGACCGAGGTTCCGATGGGAAACTCCAGGAGGTTGGCCAGGTCTCAGTGTCTCTGCAG
  AGAGCAGTGGGAGACTTCCAGACTACAAAGCTGAACGGCTTTGAGGTTTTTGCCAGGTTTGGAAGTGCCA
  TAGCTCCATTGGGAGACCTGGACCAGGATGGCTTCAATGATATTGCAATTGCTGCTCCCTATGGTGGTGA
  AGATAAGAAGGGACTTGTTTATATCTTCAATGGAAGGTCCACAGGCTTGAATTCGGTGCCATCTCAAATC
  CTCGAGGGGCAGTGGGCTGCTCAGAGCATGCCCCCAAGCTTTGGCTATTCAATGAAGGGAGCTACAGATG
  TAGACAGGAATGGATATCCAGACTTAGTTGTAGGAGCTTTTGGTGTGGATCGAGCTGTCTTATACAGAGC
  CAGACCCGTTGTCACTGTAAATGCTGGCCTTGAAGTGTACCCTAGCATTTTAAATCAAGACAATAAGATC
  TGCCCGTTGCCTGGGACAGCTCTCAAGGTTTCCTGTTTTAATGTCAGATTCTGCTTAAAGGCAGATGGCA
  AGGGAACCCTTCCTCGGAAGCTCCACTTCCAGGTGGAGCTTCTACTGGATAAACTCAAGCAGAAGGGAGC
  CATCCGACGAGCACTGTTTCTCCATAACAGGTCCCCGGTCCACTCCAAGACCATGACTGTTTTCAGGGGG
  GGACAGATGCAGTGTGAGGAACTGGTCGCCTATCTTCGGGATGAATCTGAATTTAGAGACAAGCTCACTC
  CCATCACCATTTTCATGGAGTATCGGTTGGACCAGAGAACGGCTGCTGATGCCACAGGCTTGCAGCCCAT
  CCTGAACCAGTTCACTCCGGCCAACGTCAGTCGGCAGGCTCATATTCTACTTGACTGTGGTGAAGACAAT
  GTCTGTAAACCTAAATTGGAAGTTTCTGTAAATAGTGATCAAAAGAAGATCTATATTGGGGACGACAACC
  CTCTGACACTGACTGTGAAGGCGCAGAATCAAGGGGAAGGCGCCTATGAAGCTGAGCTCATCGTTTCTAT
  CCCACCGCAGGCTGACTTCATCGGGGTTGTCCGGAACAACGAAGCCTTAGCAAGACTGTCCTGTGCATTT
  AAGACAGAAAACCAAACTCGGCAGGTGGTGTGCGACCTTGGAAACCCGATGAAGGCTGGAACTCAACTGC
  TCGCTGGTCTTCGTTTCAGCGTGCACCAGCAGTCAGAGATGGATACTTCTGTGAAGTTTGACTTGAAAAT
  CCAGAGCTCTAATTCTTTTGACAATGTAAGTCCAGTTGTGTCTTACAAGGTTGACCTCGCCGTTTTGGCT
  GCTGTGGAGATAAGAGGAGTTTCAAGTCCAGATCATATCTTTCTTCCAATTCCAAATTGGGAGTACAAGG
  AAAACCCTGAGACTGAAGAAGACGTTGGGCCTATTGTTCAGCACATATATGAGCTGAGAAACAATGGTCC
  AAGTTCATTCAGCAAGGCAATTCTAAATCTCCAGTGGCCTTACAAATACAACAACAACACTCTGTTGTAC
  ATTCTTCATTACGACATTGACGGGCCAATGAACTGCACGGCAGATACAGAGATCAACCCTTTGAGAATCA
  AGACACCCGAAAAGAATGACACAGCGGCGGCGGGACAGGGAGAAAGGAATCATCTCATCACCAAGCGGGA
  TCTCACCCTCAGAGAGGGAGATGTTCACACTTTGGGCTGTGGAATCGCCAAGTGCTTGCAGATCACCTGC
  CAGGTTGGTCGACTGGATAGAGGCAAGAGCGCAATCCTGTACGTGAAATCCCTGCTGTGGACCGAGACGT
  TCATGAACAAGGAGAACCAGAACCATTCCTATTCGCTGAAGTCATCTGCTTCCTTTAATATCATAGAATT
  CCCTTACAAGAACCTGCCGATCGAGGATCTCTTCAACTCTACACTAGTCACCACTAACATCACCTGGGGC
  ATTCAGCCGGCGCCCATGCCTGTGCCTGTGTGGGTGATCATCTTGGCAGTTCTCGCAGGGCTGCTGCTAC
  TGGCTGTGTTGGTATTTGTAATGTACAGGATGGGCTTTTTCAAACGTGTCCGACCACCTCAAGAAGAGCA
  AGAAAGAGAACAGCTTCAGCCTCATGAGAATGGCGAAGGAAACTCTGAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226884 representing NM_008402
  Red=Cloning site Green=Tags(s)
  
  MAAPGRLLLRPRPGGLLLLLPGLLLPLADAFNLDVESPAEYAGPEGSYFGFAVDFFEPSTSSRMFLLVGA
  PKANTTQPGIVEGGQVLKCECSSSRRCQPIEFDSTGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAP
  LYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSKNIDADGQGFCQGGFSIDFTKADRVLLGGPGSFYWQ
  GQLISDQVAEIISKYDPNVYSIKYNNQLATRTAQAIFDDSYLGYSVAVGDFNGDGIEDFVSGVPRAARTL
  GMVYIYDGKNMSSLHNFTGEQMAAYFGFSVAATDINGDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQ
  RAVGDFQTTKLNGFEVFARFGSAIAPLGDLDQDGFNDIAIAAPYGGEDKKGLVYIFNGRSTGLNSVPSQI
  LEGQWAAQSMPPSFGYSMKGATDVDRNGYPDLVVGAFGVDRAVLYRARPVVTVNAGLEVYPSILNQDNKI
  CPLPGTALKVSCFNVRFCLKADGKGTLPRKLHFQVELLLDKLKQKGAIRRALFLHNRSPVHSKTMTVFRG
  GQMQCEELVAYLRDESEFRDKLTPITIFMEYRLDQRTAADATGLQPILNQFTPANVSRQAHILLDCGEDN
  VCKPKLEVSVNSDQKKIYIGDDNPLTLTVKAQNQGEGAYEAELIVSIPPQADFIGVVRNNEALARLSCAF
  KTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSEMDTSVKFDLKIQSSNSFDNVSPVVSYKVDLAVLA
  AVEIRGVSSPDHIFLPIPNWEYKENPETEEDVGPIVQHIYELRNNGPSSFSKAILNLQWPYKYNNNTLLY
  ILHYDIDGPMNCTADTEINPLRIKTPEKNDTAAAGQGERNHLITKRDLTLREGDVHTLGCGIAKCLQITC
  QVGRLDRGKSAILYVKSLLWTETFMNKENQNHSYSLKSSASFNIIEFPYKNLPIEDLFNSTLVTTNITWG
  IQPAPMPVPVWVIILAVLAGLLLLAVLVFVMYRMGFFKRVRPPQEEQEREQLQPHENGEGNSET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008402
• ORF Size: 3132 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008402.3, NP_032428.2
• RefSeq Size: 7054 bp
• RefSeq ORF: 3135 bp
• Locus ID: 16410
• UniProt ID: P43406
• Cytogenetics: 2 49.33 cM
• MW: 115.8 kDa
• Gene Summary: This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 1, beta 3, beta 6 and beta 8. The heterodimer of alpha v and beta 3 forms the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. In mouse, deficiency of this gene is associated with defects in vascular morphogenesis in the brain and early post-natal death. [provided by RefSeq, May 2013]