Cd19 (NM_009844) Mouse Tagged ORF Clone

CAT#: MR226845

Cd19 (Myc-DDK-tagged) - Mouse CD19 antigen (Cd19)

Product Images

Circular map for MR226845

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd19
• Synonyms: AW495831
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226845 representing NM_009844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCTCCTCTCCCTGTCTCCTTCCTCCTCTTTCTTACCTTAGTAGGAGGCAGGCCCCAGAAGTCCT
  TACTGGTGGAGGTAGAAGAGGGAGGCAATGTTGTGCTGCCATGCCTCCCGGACTCCTCACCTGTCTCTTC
  TGAGAAGCTGGCTTGGTATCGAGGTAACCAGTCAACACCCTTCCTGGAGCTGAGCCCCGGGTCCCCTGGC
  CTGGGATTGCACGTGGGGTCCCTGGGCATCTTGCTAGTGATTGTCAATGTCTCAGACCATATGGGGGGCT
  TCTACCTGTGCCAGAAGAGGCCCCCTTTCAAGGACATCTGGCAGCCTGCCTGGACAGTGAACGTGGAGGA
  TAGTGGGGAGATGTTCCGGTGGAATGCTTCAGACGTCAGGGACCTGGACTGTGACCTAAGGAACAGGTCC
  TCTGGGAGCCACAGGTCCACTTCTGGTTCCCAGCTGTATGTGTGGGCTAAAGACCATCCTAAGGTCTGGG
  GAACAAAGCCTGTATGTGCCCCTCGGGGGAGCAGTTTGAATCAGAGTCTAATCAACCAAGATCTCACTGT
  GGCACCCGGCTCCACACTTTGGCTGTCCTGTGGGGTACCCCCTGTCCCAGTGGCCAAAGGCTCCATCTCC
  TGGACCCATGTGCATCCTAGGAGACCTAATGTTTCACTACTGAGCCTAAGCCTTGGGGGAGAGCACCCGG
  TCAGAGAGATGTGGGTTTGGGGGTCTCTTCTGCTTCTGCCCCAAGCCACAGCTTTAGATGAAGGCACCTA
  TTATTGTCTCCGAGGAAACCTGACCATCGAGAGGCACGTGAAGGTCATTGCAAGGTCAGCAGTGTGGCTC
  TGGCTGTTGAGAACTGGTGGATGGATAGTCCCAGTGGTGACTTTAGTATATGTCATCTTCTGTATGGTTT
  CTCTGGTGGCTTTTCTCTATTGTCAAAGAGCCTTTATCCTGAGAAGGAAAAGGAAGCGAATGACTGACCC
  CGCCAGGAGATTCTTCAAAGTGACGCCTCCCTCGGGAAACGGGACCCAGAACCAGTACGGGAATGTGCTC
  TCCCTTCCTACATCTACCTCTGGCCAGGCCCATGCTCAGCGTTGGGCTGCTGGCCTAGGGAGTGTCCCTG
  GGTCCTATGGAAATCCACGCATTCAAGTCCAGGATACTGGAGCTCAGAGCCATGAAACAGGACTGGAAGA
  AGAAGGGGAGGCCTATGAAGAGCCAGACAGCGAGGAGGGCTCTGAATTCTATGAGAACGACTCCAACCTT
  GGGCAGGACCAGGTTTCCCAGGATGGGAGTGGCTATGAGAACCCCGAGGATGAGCCCATGGGTCCAGAGG
  AAGAAGACTCCTTCTCCAATGCTGAGTCTTATGAAAATGCAGATGAGGAGCTGGCCCAACCAGTTGGCAG
  GATGATGGACTTCCTGAGCCCCCATGGGTCTGCGTGGGACCCCAGCCGGGAAGCATCCTCGCTTGGGTCC
  CAGTCCTATGAAGATATGAGAGGGATCCTCTATGCAGCTCCTCAGCTCCACTCAATTCAGTCCGGTCCCA
  GTCATGAAGAAGATGCAGACTCTTATGAAAACATGGATAAGTCTGACGACCTAGAACCAGCATGGGAAGG
  AGAGGGCCACATGGGGACTTGGGGAACCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226845 representing NM_009844
  Red=Cloning site Green=Tags(s)
  
  MPSPLPVSFLLFLTLVGGRPQKSLLVEVEEGGNVVLPCLPDSSPVSSEKLAWYRGNQSTPFLELSPGSPG
  LGLHVGSLGILLVIVNVSDHMGGFYLCQKRPPFKDIWQPAWTVNVEDSGEMFRWNASDVRDLDCDLRNRS
  SGSHRSTSGSQLYVWAKDHPKVWGTKPVCAPRGSSLNQSLINQDLTVAPGSTLWLSCGVPPVPVAKGSIS
  WTHVHPRRPNVSLLSLSLGGEHPVREMWVWGSLLLLPQATALDEGTYYCLRGNLTIERHVKVIARSAVWL
  WLLRTGGWIVPVVTLVYVIFCMVSLVAFLYCQRAFILRRKRKRMTDPARRFFKVTPPSGNGTQNQYGNVL
  SLPTSTSGQAHAQRWAAGLGSVPGSYGNPRIQVQDTGAQSHETGLEEEGEAYEEPDSEEGSEFYENDSNL
  GQDQVSQDGSGYENPEDEPMGPEEEDSFSNAESYENADEELAQPVGRMMDFLSPHGSAWDPSREASSLGS
  QSYEDMRGILYAAPQLHSIQSGPSHEEDADSYENMDKSDDLEPAWEGEGHMGTWGTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009844
• ORF Size: 1641 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009844.2, NP_033974.2
• RefSeq Size: 2440 bp
• RefSeq ORF: 1644 bp
• Locus ID: 12478
• UniProt ID: P25918
• Cytogenetics: 7 69.01 cM
• MW: 60.6 kDa
• Gene Summary: Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:12387743, PubMed:20101619). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:7542548, PubMed:7543183, PubMed:9317126). Required for normal differentiation of B-1 cells (PubMed:7542548, PubMed:7543183, PubMed:12387743). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:7542548, PubMed:9317126, PubMed:12387743). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:7542548, PubMed:7543183, PubMed:12387743).[UniProtKB/Swiss-Prot Function]