Jag2 (NM_010588) Mouse Tagged ORF Clone

CAT#: MR226808

Jag2 (Myc-DDK-tagged) - Mouse jagged 2 (Jag2)

Product Images

Circular map for MR226808

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jag2
• Synonyms: D12Ggc2e; mJagged2-1; Serh; sm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226808 representing NM_010588
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCACGCGGCTGGGGACGCCTGCCTCGGCGGCTGCTGCTGCTACTGGTTCTGTGCGTGCAGGCGA
  CGCGGCCCATGGGCTATTTCGAGCTGCAGCTGAGCGCGCTGCGGAACGTGAACGGGGAGCTGCTGAGCGG
  CGCCTGCTGTGACGGCGACGGCCGGACGACGCGCGCGGGGGGCTGCGGCCGCGACGAGTGCGACACGTAC
  GTGCGCGTGTGCCTTAAGGAGTACCAGGCCAAGGTGACGCCCACGGGGCCCTGCAGCTACGGCTACGGCG
  CCACGCCCGTGCTGGGTGGCAACTCCTTCTACCTGCCGCCGGCGGGCGCTGCGGGGGACCGAGCGCGCGC
  GCGGTCTCGGACCGGCGGCCACCAGGACCCGGGCCTCGTCGTCATTCCCTTTCAGTTCGCCTGGCCGCGT
  TCTTTCACCCTCATCGTGGAGGCCTGGGACTGGGACAATGACACCACTCCAGATGAGGAGCTGCTGATTG
  AGCGGGTGTCGCACGCTGGCATGATCAACCCCGAGGACCGCTGGAAGAGCCTGCACTTCAGCGGCCACGT
  GGCACACCTGGAGCTGCAGATCCGAGTACGCTGTGATGAGAACTACTACAGTGCCACCTGCAACAAGTTC
  TGCCGGCCCCGCAACGACTTCTTTGGCCACTATACCTGCGACCAGTACGGCAACAAGGCCTGCATGGATG
  GCTGGATGGGCAAAGAATGCAAAGAAGCCGTGTGTAAACAAGGATGTAATTTGCTCCACGGGGGATGCAC
  TGTGCCTGGGGAGTGCAGGTGCAGCTACGGCTGGCAGGGCAAGTTCTGTGACGAGTGTGTCCCCTACCCT
  GGCTGCGTGCATGGCAGCTGTGTGGAGCCCTGGCACTGTGACTGTGAGACCAACTGGGGTGGCCTGCTCT
  GTGACAAAGACCTGAACTACTGTGGCAGCCACCACCCCTGTGTCAACGGGGGTACCTGCATCAATGCTGA
  GCCTGACCAATACCTCTGCGCCTGCCCAGATGGCTACTTGGGCAAGAACTGCGAGCGGGCTGAGCACGCC
  TGTGCCTCCAACCCGTGTGCCAATGGGGGCTCTTGCCACGAAGTGCCATCTGGCTTTGAATGCCACTGTC
  CGTCAGGATGGAACGGACCCACCTGTGCGCTCGACATTGATGAGTGTGCCTCTAACCCATGTGCAGCGGG
  TGGTACCTGCGTGGATCAGGTGGACGGCTTCGAGTGCATCTGCCCGGAGCAGTGGGTGGGGGCTACTTGC
  CAGCTGGACGCCAATGAGTGTGAAGGGAAGCCGTGCCTTAATGCTTTTTCTTGCAAAAACCTGATTGGCG
  GCTATTACTGTGATTGCCTCCCGGGCTGGAAGGGCATCAACTGCCAAATCAACATCAACGATTGCCATGG
  GCAGTGTCAGCATGGGGGCACCTGCAAGGACCTGGTCAATGGGTACCAGTGTGTGTGCCCGCGGGGCTTT
  GGAGGTCGCCATTGCGAACTAGAGTACGACAAGTGTGCCAGCAGCCCCTGCCGCCGGGGTGGCATCTGTG
  AGGACCTGGTGGATGGCTTCCGCTGCCACTGCCCACGGGGCCTCTCTGGGCTGCACTGTGAGGTGGACAT
  GGATCTCTGTGAACCAAGCCCCTGCCTCAACGGTGCTCGCTGCTACAACCTTGAGGGTGACTACTACTGC
  GCCTGCCCAGAAGACTTTGGTGGCAAGAACTGCTCAGTGCCCAGGGACACATGCCCTGGCGGGGCATGTA
  GAGTGATCGATGGCTGCGGGTTCGAGGCAGGGTCCAGGGCACGCGGTGTCGCACCCTCTGGTATATGTGG
  CCCTCACGGGCACTGCGTTAGCCTGCCTGGGGGAAACTTCTCCTGCATCTGTGACAGCGGCTTCACAGGC
  ACCTACTGCCATGAAAACATTGACGACTGCATGGGCCAGCCCTGCCGCAACGGGGGCACGTGCATTGACG
  AAGTGGACTCCTTCCGCTGCTTCTGCCCCAGTGGCTGGGAAGGAGAACTCTGTGACATCAATCCCAACGA
  CTGCCTCCCCGACCCCTGCCACAGCCGCGGCCGCTGCTATGACCTGGTCAATGACTTCTACTGTGCCTGT
  GACGATGGCTGGAAGGGCAAGACCTGCCACTCACGCGAGTTCCAGTGTGACGCCTACACCTGCAGCAACG
  GTGGCACATGCTATGACAGCGGCGACACCTTCCGCTGCGCGTGCCCTCCGGGCTGGAAGGGCAGCACCTG
  CACCATCGCCAAGAACAGCAGCTGTGTGCCCAATCCCTGTGTGAATGGAGGCACCTGCGTGGGCAGCGGA
  GACTCTTTCTCCTGCATCTGCCGGGATGGCTGGGAGGGCCGCACCTGCACACATAACACCAATGACTGCA
  ACCCTCTGCCCTGCTATAACGGAGGCATCTGTGTTGATGGCGTCAACTGGTTCCGCTGCGAGTGTGCGCC
  TGGCTTTGCGGGTCCTGACTGCCGTATCAACATTGATGAGTGCCAGTCCTCGCCCTGTGCCTACGGAGCC
  ACGTGTGTGGATGAGATCAACGGGTACCGCTGCAGCTGCCCACCAGGTCGTTCTGGCCCCAGGTGCCAGG
  AAGTGGTCATATTCACGAGGCCCTGCTGGTCCCGGGGAATGTCCTTCCCGCATGGGAGTTCCTGGATGGA
  AGACTGCAACAGCTGCCGCTGCCTGGATGGCCACCGGGATTGTAGCAAGGTATGGTGCGGATGGAAGCCT
  TGCCTGCTCTCTGGTCAGCCCAGCGATCCGAGTGCCCAGTGCCCCCCAGGGCAGCAATGTCAGGAGAAGG
  CCGTGGGTCAGTGCTTGCAGCCACCCTGTGAGAACTGGGGGGAGTGTACAGCGGAGGAGCCTCTGCCACC
  CAGCACCCCCTGTCAGCCACGGAGCAGTCATTTGGACAACAACTGTGCCCGACTCACACTGCGCTTCAAC
  CGTGATCAAGTGCCTCAGGGCACCACCGTGGGCGCTATCTGCTCTGGAATCCGAGCCTTGCCTGCCACGA
  GGGCGGCGGCACACGACCGCCTCCTCCTGCTGCTTTGTGATCGAGCATCCTCGGGGGCCAGTGCTGTGGA
  GGTGGCTGTGTCTTTCAGCCCTGCAAGGGACCTGCCTGACAGCAGCCTGATCCAGAGCACAGCCCACGCC
  ATCGTGGCTGCTATCACTCAGAGAGGAAATAGCTCACTGCTGCTGGCTGTCACCGAGGTCAAGGTGGAAA
  CAGTTGTTATGGGTGGCTCTTCCACAGGTCTGTTGGTGCCCGTGCTGTGCAGCGTGTTCAGTGTGCTGTG
  GCTCGCCTGTGTGGTTATCTGCGTATGGTGGACACGAAAGCGCAGGAAAGAACGTGAGAGGAGCCGGCTA
  CCACGGGATGAGAGCGCCAACAACCAGTGGGCCCCGCTCAATCCCATCCGCAACCCCATTGAGCGGCCAG
  GCGGCAGCGGTCTGGGAACTGGGGGCCACAAGGACATACTCTACCAGTGCAAAAACTTCACACCGCCGCC
  CCGCAGGGCAGGCGAGGCACTGCCCGGGCCAGCTGGCCATGGGGCTGGTGGGGAGGACGAGGAGGATGAA
  GAGCTGAGCCGTGGAGATGGGGACTCCCCAGAGGCAGAGAAGTTCATCTCACACAAGTTCACCAAAGACC
  CCAGCTGCTCCCTCGGAAGGCCAGCCCGCTGGGCTCCAGGGCCCAAAGTGGACAACCGCGCCGTCAGAAG
  TACCAAGGACGTGCGCCGTGCTGGCAGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226808 representing NM_010588
  Red=Cloning site Green=Tags(s)
  
  MRARGWGRLPRRLLLLLVLCVQATRPMGYFELQLSALRNVNGELLSGACCDGDGRTTRAGGCGRDECDTY
  VRVCLKEYQAKVTPTGPCSYGYGATPVLGGNSFYLPPAGAAGDRARARSRTGGHQDPGLVVIPFQFAWPR
  SFTLIVEAWDWDNDTTPDEELLIERVSHAGMINPEDRWKSLHFSGHVAHLELQIRVRCDENYYSATCNKF
  CRPRNDFFGHYTCDQYGNKACMDGWMGKECKEAVCKQGCNLLHGGCTVPGECRCSYGWQGKFCDECVPYP
  GCVHGSCVEPWHCDCETNWGGLLCDKDLNYCGSHHPCVNGGTCINAEPDQYLCACPDGYLGKNCERAEHA
  CASNPCANGGSCHEVPSGFECHCPSGWNGPTCALDIDECASNPCAAGGTCVDQVDGFECICPEQWVGATC
  QLDANECEGKPCLNAFSCKNLIGGYYCDCLPGWKGINCQININDCHGQCQHGGTCKDLVNGYQCVCPRGF
  GGRHCELEYDKCASSPCRRGGICEDLVDGFRCHCPRGLSGLHCEVDMDLCEPSPCLNGARCYNLEGDYYC
  ACPEDFGGKNCSVPRDTCPGGACRVIDGCGFEAGSRARGVAPSGICGPHGHCVSLPGGNFSCICDSGFTG
  TYCHENIDDCMGQPCRNGGTCIDEVDSFRCFCPSGWEGELCDINPNDCLPDPCHSRGRCYDLVNDFYCAC
  DDGWKGKTCHSREFQCDAYTCSNGGTCYDSGDTFRCACPPGWKGSTCTIAKNSSCVPNPCVNGGTCVGSG
  DSFSCICRDGWEGRTCTHNTNDCNPLPCYNGGICVDGVNWFRCECAPGFAGPDCRINIDECQSSPCAYGA
  TCVDEINGYRCSCPPGRSGPRCQEVVIFTRPCWSRGMSFPHGSSWMEDCNSCRCLDGHRDCSKVWCGWKP
  CLLSGQPSDPSAQCPPGQQCQEKAVGQCLQPPCENWGECTAEEPLPPSTPCQPRSSHLDNNCARLTLRFN
  RDQVPQGTTVGAICSGIRALPATRAAAHDRLLLLLCDRASSGASAVEVAVSFSPARDLPDSSLIQSTAHA
  IVAAITQRGNSSLLLAVTEVKVETVVMGGSSTGLLVPVLCSVFSVLWLACVVICVWWTRKRRKERERSRL
  PRDESANNQWAPLNPIRNPIERPGGSGLGTGGHKDILYQCKNFTPPPRRAGEALPGPAGHGAGGEDEEDE
  ELSRGDGDSPEAEKFISHKFTKDPSCSLGRPARWAPGPKVDNRAVRSTKDVRRAGRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010588
• ORF Size: 3741 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010588.2, NP_034718.2
• RefSeq Size: 4054 bp
• RefSeq ORF: 3744 bp
• Locus ID: 16450
• UniProt ID: Q9QYE5
• Cytogenetics: 12 61.37 cM
• MW: 135.2 kDa
• Gene Summary: Putative Notch ligand involved in the mediation of Notch signaling. Plays an essential role during limb, craniofacial and thymic development. May be involved in myogenesis and in the development of peripheral and central nervous systems.[UniProtKB/Swiss-Prot Function]