Ctgf (NM_010217) Mouse Tagged ORF Clone

CAT#: MR226802

  • TrueORF®

Ctgf (Myc-DDK-tagged) - Mouse connective tissue growth factor (Ctgf)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_010217" in other vectors (3)


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Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit Polyclonal Antibody against CTGF
    • 100 ul

USD 457.00

Other products for "Ctgf"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ctgf
Synonyms Ccn2; fisp-12; Fisp12; Hcs24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226802 representing NM_010217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCGCCTCCGTCGCAGGTCCCATCAGCCTCGCCTTGGTGCTCCTCGCCCTCTGCACCCGGCCTGCTA
CGGGCCAGGACTGCAGCGCGCAATGTCAGTGCGCAGCCGAAGCAGCGCCGCACTGCCCCGCCGGCGTGAG
CCTGGTGCTGGACGGCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGAGAACTGTGTACGGAGCGT
GACCCCTGCGACCCACACAAGGGCCTCTTCTGCGATTTCGGCTCCCCCGCCAACCGCAAGATCGGAGTGT
GCACTGCCAAAGATGGTGCACCCTGTGTCTTCGGTGGGTCGGTGTACCGCAGCGGTGAGTCCTTCCAAAG
CAGCTGCAAATACCAATGCACTTGCCTGGATGGGGCCGTGGGCTGCGTGCCCCTGTGCAGCATGGACGTG
CGCCTGCCCAGCCCTGACTGCCCCTTCCCGAGAAGGGTCAAGCTGCCTGGGAAATGCTGCGAGGAGTGGG
TGTGTGACGAGCCCAAGGACCGCACAGCAGTTGGCCCTGCCCTAGCTGCCTACCGACTGGAAGACACATT
TGGCCCAGACCCAACTATGATGCGAGCCAACTGCCTGGTCCAGACCACAGAGTGGAGCGCCTGTTCTAAG
ACCTGTGGGATGGGCATCTCCACCCGAGTTACCAATGACAATACCTTCTGCAGACTTGAGAAGCAGAGTC
GCCTCTGCATGGTCAGGCCCTGCGAAGCTGACCTGGAGGAAAACATTAAGAAGGGCAAAAAGTGCATCCG
GACACCTAAAATCGCCAAGCCTGTCAAGTTTGAGCTTTCTGGCTGCACCAGTGTGAAGACATACAGGGCT
AAGTTCTGCGGGGTGTGCACAGACGGCCGCTGCTGCACACCGCACAGAACCACCACTCTGCCAGTGGAGT
TCAAATGCCCCGATGGCGAGATCATGAAAAAGAATATGATGTTCATCAAGACCTGTGCCTGCCATTACAA
CTGTCCTGGGGACAATGACATCTTTGAGTCCCTGTACTACAGGAAGATGTACGGAGACATGGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226802 representing NM_010217
Red=Cloning site Green=Tags(s)

MLASVAGPISLALVLLALCTRPATGQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTER
DPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDV
RLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSK
TCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRA
KFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010217
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010217.1, NM_010217.2, NP_034347.2
RefSeq Size 2418 bp
RefSeq ORF 1047 bp
Locus ID 14219
UniProt ID P29268
Cytogenetics 10 11.84 cM
MW 38.3 kDa
Gene Summary Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).[UniProtKB/Swiss-Prot Function]

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