Ctgf (NM_010217) Mouse Tagged ORF Clone
CAT#: MR226802
- TrueORF®
Ctgf (Myc-DDK-tagged) - Mouse connective tissue growth factor (Ctgf)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010217" in other vectors (3)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ctgf |
Synonyms | Ccn2; fisp-12; Fisp12; Hcs24 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226802 representing NM_010217
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCTCCGTCGCAGGTCCCATCAGCCTCGCCTTGGTGCTCCTCGCCCTCTGCACCCGGCCTGCTA CGGGCCAGGACTGCAGCGCGCAATGTCAGTGCGCAGCCGAAGCAGCGCCGCACTGCCCCGCCGGCGTGAG CCTGGTGCTGGACGGCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGAGAACTGTGTACGGAGCGT GACCCCTGCGACCCACACAAGGGCCTCTTCTGCGATTTCGGCTCCCCCGCCAACCGCAAGATCGGAGTGT GCACTGCCAAAGATGGTGCACCCTGTGTCTTCGGTGGGTCGGTGTACCGCAGCGGTGAGTCCTTCCAAAG CAGCTGCAAATACCAATGCACTTGCCTGGATGGGGCCGTGGGCTGCGTGCCCCTGTGCAGCATGGACGTG CGCCTGCCCAGCCCTGACTGCCCCTTCCCGAGAAGGGTCAAGCTGCCTGGGAAATGCTGCGAGGAGTGGG TGTGTGACGAGCCCAAGGACCGCACAGCAGTTGGCCCTGCCCTAGCTGCCTACCGACTGGAAGACACATT TGGCCCAGACCCAACTATGATGCGAGCCAACTGCCTGGTCCAGACCACAGAGTGGAGCGCCTGTTCTAAG ACCTGTGGGATGGGCATCTCCACCCGAGTTACCAATGACAATACCTTCTGCAGACTTGAGAAGCAGAGTC GCCTCTGCATGGTCAGGCCCTGCGAAGCTGACCTGGAGGAAAACATTAAGAAGGGCAAAAAGTGCATCCG GACACCTAAAATCGCCAAGCCTGTCAAGTTTGAGCTTTCTGGCTGCACCAGTGTGAAGACATACAGGGCT AAGTTCTGCGGGGTGTGCACAGACGGCCGCTGCTGCACACCGCACAGAACCACCACTCTGCCAGTGGAGT TCAAATGCCCCGATGGCGAGATCATGAAAAAGAATATGATGTTCATCAAGACCTGTGCCTGCCATTACAA CTGTCCTGGGGACAATGACATCTTTGAGTCCCTGTACTACAGGAAGATGTACGGAGACATGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226802 representing NM_010217
Red=Cloning site Green=Tags(s) MLASVAGPISLALVLLALCTRPATGQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTER DPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDV RLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSK TCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRA KFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010217 |
ORF Size | 1044 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010217.1, NM_010217.2, NP_034347.2 |
RefSeq Size | 2418 bp |
RefSeq ORF | 1047 bp |
Locus ID | 14219 |
UniProt ID | P29268 |
Cytogenetics | 10 11.84 cM |
MW | 38.3 kDa |
Gene Summary | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226802 | Ctgf (tGFP-tagged) - Mouse connective tissue growth factor (Ctgf), (10ug) |
USD 657.00 |
|
MR226802L3 | Lenti ORF clone of Ctgf (Myc-DDK-tagged) - Mouse connective tissue growth factor (Ctgf) |
USD 757.00 |
|
MR226802L4 | Lenti ORF clone of Ctgf (mGFP-tagged) - Mouse connective tissue growth factor (Ctgf) |
USD 757.00 |
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