Cd4 (NM_013488) Mouse Tagged ORF Clone

CAT#: MR226754

Cd4 (Myc-DDK-tagged) - Mouse CD4 antigen (Cd4)

Product Images

Circular map for MR226754

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd4
• Synonyms: L3T4; Ly-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226754 representing NM_013488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCGAGCCATCTCTCTTAGGCGCTTGCTGCTGCTGCTGCTGCAGCTGTCACAACTCCTAGCTGTCA
  CTCAAGGGAAGACGCTGGTGCTGGGGAAGGAAGGGGAATCAGCAGAACTGCCCTGCGAGAGTTCCCAGAA
  GAAGATCACAGTCTTCACCTGGAAGTTCTCTGACCAGAGGAAGATTCTGGGGCAGCATGGCAAAGGTGTA
  TTAATTAGAGGAGGTTCGCCTTCGCAGTTTGATCGTTTTGATTCCAAAAAAGGGGCATGGGAGAAAGGAT
  CGTTTCCTCTCATCATCAATAAACTTAAGATGGAAGACTCTCAGACTTATATCTGTGAGCTGGAGAACAG
  GAAAGAGGAGGTGGAGTTGTGGGTGTTCAAAGTGACCTTCAGTCCGGGTACCAGCCTGTTGCAAGGGCAG
  AGCCTGACCCTGACCTTGGATAGCAACTCTAAGGTCTCTAACCCCTTGACAGAGTGCAAACACAAAAAGG
  GTAAAGTTGTCAGTGGTTCCAAAGTTCTCTCCATGTCCAACCTAAGGGTTCAGGACAGCGACTTCTGGAA
  CTGCACCGTGACCCTGGACCAGAAAAAGAACTGGTTCGGCATGACACTCTCAGTGCTGGGTTTTCAGAGC
  ACAGCTATCACGGCCTATAAGAGTGAGGGAGAGTCAGCGGAGTTCTCCTTCCCACTCAACTTTGCAGAGG
  AAAACGGGTGGGGAGAGCTGATGTGGAAGGCAGAGAAGGATTCTTTCTTCCAGCCCTGGATCTCCTTCTC
  CATAAAGAACAAAGAGGTGTCCGTACAAAAGTCCACCAAAGACCTCAAGCTCCAGCTGAAGGAAACGCTC
  CCACTCACCCTCAAGATACCCCAGGTCTCGCTTCAGTTTGCTGGTTCTGGCAACCTGACTCTGACTCTGG
  ACAAAGGGACACTGCATCAGGAAGTGAACCTGGTGGTGATGAAAGTGGCTCAGCTCAACAATACTTTGAC
  CTGTGAGGTGATGGGACCTACCTCTCCCAAGATGAGACTGACCCTGAAGCAGGAGAACCAGGAGGCCAGG
  GTCTCTGAGGAGCAGAAAGTAGTTCAAGTGGTGGCCCCTGAGACAGGGCTGTGGCAGTGTCTACTGAGTG
  AAGGTGATAAGGTCAAGATGGACTCCAGGATCCAGGTTTTATCCAGAGGGGTGAACCAGACAGTGTTCCT
  GGCTTGCGTGCTGGGTGGCTCCTTCGGCTTTCTGGGTTTCCTTGGGCTCTGCATCCTCTGCTGTGTCAGG
  TGCCGGCACCAACAGCGCCAGGCAGCACGAATGTCTCAGATCAAGAGGCTCCTCAGTGAGAAGAAGACCT
  GCCAGTGCCCCCACCGGATGCAGAAGAGCCATAATCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226754 representing NM_013488
  Red=Cloning site Green=Tags(s)
  
  MCRAISLRRLLLLLLQLSQLLAVTQGKTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGV
  LIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQ
  SLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQS
  TAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETL
  PLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEAR
  VSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQTVFLACVLGGSFGFLGFLGLCILCCVR
  CRHQQRQAARMSQIKRLLSEKKTCQCPHRMQKSHNLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013488
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013488.3
• RefSeq Size: 3095 bp
• RefSeq ORF: 1374 bp
• Locus ID: 12504
• UniProt ID: P06332
• Cytogenetics: 6 59.17 cM
• MW: 51.7 kDa
• Gene Summary: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.[UniProtKB/Swiss-Prot Function]