Tcf7 (NM_009331) Mouse Tagged ORF Clone

CAT#: MR226713

Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tcf7
• Synonyms: AI465550; TCF-1; Tcf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226713 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAAAGAGACTGTCTACTCTGCCTTCAATCTGCTCATGCCCTACCCACCGGCCTCGGGAGCAGGGC
  AGCATCCGCAGCCTCAACCCCCGCTGCATAACAAGCCCGGCCAGCCTCCACATGGCGTCCCCCAGCTTTC
  TCCACTCTACGAACATTTCAGCAGTCCACACCCCACCCCTGCACCAGCGGACATCAGCCAGAAGCAAGGA
  GTTCACAGGCCGCTGCAGACCCCCGATCTCTCTGGATTTTATTCTCTGACCTCAGGCAGCATGGGACAGC
  TCCCCCATACTGTGAGCTGGCCCAGTCCTCCTCTCTACCCCCTGTCCCCTTCCTGCGGATATAGACAGCA
  CTTCCCTGCCCCCACTGCAGCCCCTGGCGCCCCCTATCCCAGGTTCACCCACCCATCCTTGATGCTGGGA
  TCTGGTGTACCTGGACACCCAGCAGCCATCCCTCACCCGGCCATTGTGCCCTCCTCAGGGAAGCAGGAGC
  TGCAGCCATATGATAGAAACCTGAAAACACAGGCAGAACCCAAGGCAGAGAAGGAGGCTAAGAAGCCAGT
  CATCAAGAAACCCCTCAATGCGTTCATGCTTTACATGAAGGAGATGAGAGCCAAGGTCATTGCTGAGTGC
  ACACTCAAGGAGAGCGCTGCCATCAACCAGATCCTGGGTCGCAGGTGGCATGCACTATCTCGAGAAGAGC
  AGGCCAAGTACTATGAACTGGCCCGCAAGGAAAGGCAGCTGCACATGCAGCTATACCCGGGCTGGTCCGC
  GCGGGATAACTACGGAAAGAAGAAGAGGCGGTCAAGGGAAAAACATCAAGAATCCACCACAGGAGGAAAA
  AGAAATGCATTCGGTACTTACCCGGAGAAGGCCGCTGCCCCAGCCCCGTTCCTTCCGATGACAGTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226713 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYKETVYSAFNLLMPYPPASGAGQHPQPQPPLHNKPGQPPHGVPQLSPLYEHFSSPHPTPAPADISQKQG
  VHRPLQTPDLSGFYSLTSGSMGQLPHTVSWPSPPLYPLSPSCGYRQHFPAPTAAPGAPYPRFTHPSLMLG
  SGVPGHPAAIPHPAIVPSSGKQELQPYDRNLKTQAEPKAEKEAKKPVIKKPLNAFMLYMKEMRAKVIAEC
  TLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTGGK
  RNAFGTYPEKAAAPAPFLPMTVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009331
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009331.1
• RefSeq Size: 2238 bp
• RefSeq ORF: 912 bp
• Locus ID: 21414
• UniProt ID: Q00417
• Cytogenetics: 11 31.86 cM
• MW: 33.6 kDa
• Gene Summary: This gene encodes a transcription factor which is a member of the T-cell specific transcription factor family. The encoded protein is distinct from the hepatic transcription factor, transcription factor 1, which is also referred to by the symbol Tcf1. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015]