Itgb2 (NM_008404) Mouse Tagged ORF Clone

CAT#: MR226636

Itgb2 (Myc-DDK-tagged) - Mouse integrin beta 2 (Itgb2)

Product Images

Circular map for MR226636

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgb2
• Synonyms: 2E6; AI528527; Cd18; LAD; LCAMB; Lfa1; MF17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226636 representing NM_008404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCCCACACTCACTGCTGCTTGCCCTAGCTGGACTGTTCTTCCTGGGATCTGCTGTGTCCCAGG
  AATGCACCAAGTACAAAGTCAGCAGTTGCCGGGACTGTATCCAGTCGGGGCCTGGCTGTTCCTGGTGCCA
  GAAGCTGAACTTCACTGGACCAGGAGAACCTGACTCCTTGCGCTGTGACACACGGGCACAGCTGCTGCTG
  AAGGGTTGTCCAGCCGATGATATCATGGACCCCAGGAGCATCGCTAATCCTGAGTTCGACCAACGGGGGC
  AACGGAAACAGCTATCTCCACAAAAAGTGACACTTTACTTGCGACCAGGACAGGCTGCCGCATTCAATGT
  GACTTTCCGGCGGGCCAAGGGATACCCCATTGATCTGTACTACCTCATGGATCTCTCCTACTCCATGCTT
  GATGACCTCAACAACGTCAAGAAGCTGGGCGGGGACTTGCTGCAGGCCCTCAACGAGATCACCGAGTCTG
  GCCGCATCGGCTTTGGGTCGTTTGTGGACAAGACGGTGCTGCCTTTTGTTAACACCCATCCTGAGAAGCT
  GAGGAACCCATGTCCCAACAAGGAGAAGGCCTGCCAGCCCCCATTTGCCTTTCGGCACGTGCTCAAGTTA
  ACCGACAACTCCAACCAGTTTCAGACAGAGGTCGGCAAGCAACTGATTTCCGGAAACCTGGACGCCCCTG
  AGGGTGGGCTGGATGCCATAATGCAAGTTGCTGCATGTCCGGAGGAAATTGGCTGGCGCAATGTCACGAG
  GCTGCTGGTGTTTGCCACAGACGATGGCTTCCACTTTGCTGGTGATGGCAAACTGGGTGCCATCCTGACC
  CCCAATGATGGCCGCTGCCACCTGGAGGATAACATGTACAAGAGGAGCAATGAGTTCGACTACCCATCCG
  TGGGTCAGCTGGCACACAAACTTTCCGAGAGCAACATCCAGCCCATCTTTGCGGTGACAAAGAAGATGGT
  GAAAACGTATGAGAAACTCACGGAGATCATCCCCAAGTCAGCAGTGGGGGAACTGTCTGACGACTCCAGC
  AACGTGGTGCAGCTCATCAAGAATGCCTACTATAAACTCTCCTCTAGAGTCTTCCTGGACCACAGCACCC
  TCCCGGACACCCTGAAAGTCACCTATGACTCCTTCTGCAGTAATGGAGCATCGAGTATAGGCAAATCCCG
  TGGGGACTGTGATGGCGTACAGATCAACAACCCGGTCACCTTCCAGGTAAAGGTCATGGCTTCCGAGTGT
  ATCCAGGAGCAGTCCTTTGTCATCCGGGCACTGGGTTTCACGGATACAGTGACCGTGCAGGTCCGTCCCC
  AGTGTGAGTGTCAGTGCCGGGACCAGAGTCGGGAGCAGAGTCTCTGTGGAGGCAAGGGAGTCATGGAGTG
  TGGTATCTGCAGGTGTGAGTCTGGCTACATTGGGAAAAACTGTGAGTGCCAGACTCAGGGTCGGAGCAGC
  CAGGAGCTGGAGAGAAACTGTCGGAAGGACAATAGTTCCATCGTGTGCTCAGGGCTTGGGGACTGCATCT
  GTGGGCAGTGTGTATGCCATACCAGTGACGTCCCCAACAAAGAGATCTTTGGGCAATACTGCGAGTGTGA
  CAATGTCAACTGTGAGAGATATAACAGCCAAGTCTGCGGTGGCTCAGATCGGGGTTCCTGCAACTGTGGC
  AAATGTAGTTGCAAGCCCGGTTACGAGGGCTCGGCCTGCCAGTGTCAGAGGTCCACCACGGGCTGTCTGA
  ATGCACGGCTGGTAGAGTGCAGTGGCCGTGGCCACTGCCAATGCAACAGGTGCATATGTGACGAAGGCTA
  CCAGCCACCGATGTGTGAGGATTGTCCCAGCTGTGGCTCGCACTGCAGGGACAACCACACCTCTTGTGCC
  GAGTGCCTGAAGTTTGATAAGGGCCCTTTTGAGAAGAACTGTAGTGTTCAGTGTGCTGGTATGACGCTGC
  AGACTATCCCTTTGAAGAAAAAGCCCTGCAAGGAGAGGGACTCGGAAGGCTGTTGGATAACTTACACTTT
  GCAGCAGAAGGACGGAAGGAACATTTACAACATCCATGTGGAGGACAGTCTAGAGTGTGTGAAGGGCCCC
  AATGTGGCTGCCATCGTAGGGGGCACCGTGGTAGGTGTCGTACTGATTGGTGTCCTCCTCCTGGTCATCT
  GGAAGGCCCTGACCCACCTGACTGACCTCAGGGAGTACAGGCGCTTTGAGAAGGAGAAACTCAAGTCCCA
  ATGGAACAATGACAACCCCCTCTTCAAGAGTGCTACGACAACGGTCATGAACCCCAAGTTTGCTGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226636 representing NM_008404
  Red=Cloning site Green=Tags(s)
  
  MLGPHSLLLALAGLFFLGSAVSQECTKYKVSSCRDCIQSGPGCSWCQKLNFTGPGEPDSLRCDTRAQLLL
  KGCPADDIMDPRSIANPEFDQRGQRKQLSPQKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSML
  DDLNNVKKLGGDLLQALNEITESGRIGFGSFVDKTVLPFVNTHPEKLRNPCPNKEKACQPPFAFRHVLKL
  TDNSNQFQTEVGKQLISGNLDAPEGGLDAIMQVAACPEEIGWRNVTRLLVFATDDGFHFAGDGKLGAILT
  PNDGRCHLEDNMYKRSNEFDYPSVGQLAHKLSESNIQPIFAVTKKMVKTYEKLTEIIPKSAVGELSDDSS
  NVVQLIKNAYYKLSSRVFLDHSTLPDTLKVTYDSFCSNGASSIGKSRGDCDGVQINNPVTFQVKVMASEC
  IQEQSFVIRALGFTDTVTVQVRPQCECQCRDQSREQSLCGGKGVMECGICRCESGYIGKNCECQTQGRSS
  QELERNCRKDNSSIVCSGLGDCICGQCVCHTSDVPNKEIFGQYCECDNVNCERYNSQVCGGSDRGSCNCG
  KCSCKPGYEGSACQCQRSTTGCLNARLVECSGRGHCQCNRCICDEGYQPPMCEDCPSCGSHCRDNHTSCA
  ECLKFDKGPFEKNCSVQCAGMTLQTIPLKKKPCKERDSEGCWITYTLQQKDGRNIYNIHVEDSLECVKGP
  NVAAIVGGTVVGVVLIGVLLLVIWKALTHLTDLREYRRFEKEKLKSQWNNDNPLFKSATTTVMNPKFAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008404
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008404.5
• RefSeq Size: 2862 bp
• RefSeq ORF: 2313 bp
• Locus ID: 16414
• UniProt ID: P11835
• Cytogenetics: 10 39.72 cM
• MW: 85.3 kDa
• Gene Summary: Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (By similarity). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (By similarity). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (By similarity). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (By similarity). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). Integrin ITGAM/ITGB2 plays a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the ITGAM subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.[UniProtKB/Swiss-Prot Function]