Foxo3 (NM_019740) Mouse Tagged ORF Clone

CAT#: MR226631

Foxo3 (Myc-DDK-tagged) - Mouse forkhead box O3 (Foxo3)

Product Images

Circular map for MR226631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxo3
• Synonyms: 1110048B16Rik; 2010203A17Rik; C76856; Fkhr2; FKHRL1; Foxo3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226631 representing NM_019740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGCACCAGCCTCCCCGGTCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCAGAGTTCGAGC
  CACAGAGTCGGCCACGCTCCTGTACGTGGCCCCTGCAGAGGCCGGAGCTGCAGGCGAGCCCGGCCAAGCC
  CTCGGGGGAGACGGCCGCAGACTCCATGATCCCCGAGGAGGACGACGATGAAGACGACGAGGACGGCGGC
  GGCCGAGCCAGCTCGGCCATGGTGATCGGTGGCGGCGTGAGCAGCACGCTGGGTTCCGGGCTGCTCCTCG
  AGGATTCGGCCATGCTGCTGGCTCCAGGAGGGCAGGACCTCGGGTCGGGGCCAGCGTCCGCCGCAGGCGC
  TCTGAGTGGGGGCACGCCGACGCAGCTGCAGCCTCAGCAGCCACTGCCACAGCCGCAGCCGGGGGCGGCT
  GGGGGCTCTGGGCAACCAAGGAAATGCTCCTCGCGGCGGAATGCCTGGGGGAACCTGTCCTATGCCGACC
  TGATCACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTTTGTCCCAGATCTACGAGTGGATGGT
  GCGCTGTGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCGGGCTGGAAGAACTCCATCCGG
  CACAACCTGTCCCTGCACAGCCGCTTCATGCGCGTTCAGAATGAAGGCACGGGCAAGAGCTCTTGGTGGA
  TCATCAACCCCGATGGGGGAAAGAGCGGGAAGGCCCCCCGGCGGCGTGCGGTCTCCATGGACAACAGCAA
  CAAGTACACCAAGAGCCGAGGCCGGGCAGCCAAGAAGAAGGCGGCCCTGCAGGCTGCCCCAGAGTCGGCA
  GACGACAGTCCTTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCCCGCAGCAGCGACGAGCTGGATG
  CGTGGACCGACTTCCGCTCGCGCACCAATTCCAACGCCAGCACCGTGAGCGGCCGCCTGTCGCCCATCCT
  GGCAAGCACGGAGCTGGATGACGTCCAGGATGATGATGGACCCCTGTCCCCCATGCTGTACAGCAGCTCT
  GCCAGCCTGTCGCCCTCCGTGAGCAAGCCGTGTACTGTGGAGCTTCCGCGGCTGACGGACATGGCCGGCA
  CCATGAATCTGAATGATGGGCTGGCCGAGAACCTCATGGACGACCTGCTGGATAACATCGCGCTCCCGCC
  ATCGCAGCCATCGCCTCCTGGCGGGCTTATGCAGCGGGGCTCCAGCTTCCCATATACCGCCAAGAGCTCC
  GGCCTGGGCTCCCCAACCGGCTCCTTCAACAGTACCGTGTTTGGACCTTCGTCTCTGAACTCCTTGCGTC
  AGTCACCCATGCAGACTATCCAGGAGAACAGACCAGCCACCTTCTCTTCCGTGTCACACTACGGCAACCA
  GACACTCCAAGACCTGCTTGCTTCAGACTCACTCAGCCACAGCGACGTCATGATGACCCAGTCGGACCCC
  TTGATGTCTCAGGCTAGCACCGCCGTGTCCGCCCAGAATGCCCGCCGGAACGTGATGCTTCGCAACGATC
  CAATGATGTCCTTTGCTGCCCAGCCTACCCAGGGGAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCA
  CCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCAAATTCTGTCAGCAACATGGGCTTGAGTGAC
  TCCAGCAGCCTTGGCTCAGCCAAACACCAGCAGCAGTCTCCCGCCAGCCAGTCTATGCAAACCCTCTCGG
  ACTCTCTCTCAGGCTCCTCACTGTATTCAGCTAGTGCAAACCTTCCCGTCATGGGCCACGATAAGTTCCC
  CAGTGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAATGTGACATGGAGTCCATCATCCGTAGTGAA
  CTCATGGATGCTGACGGGTTGGATTTTAACTTTGACTCCCTCATCTCCACACAGAACGTTGTTGGTTTGA
  ATGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAAAGCTGGGTACCAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226631 representing NM_019740
  Red=Cloning site Green=Tags(s)
  
  MAEAPASPVPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEDDDEDDEDGG
  GRASSAMVIGGGVSSTLGSGLLLEDSAMLLAPGGQDLGSGPASAAGALSGGTPTQLQPQQPLPQPQPGAA
  GGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIR
  HNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQAAPESA
  DDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPILASTELDDVQDDDGPLSPMLYSSS
  ASLSPSVSKPCTVELPRLTDMAGTMNLNDGLAENLMDDLLDNIALPPSQPSPPGGLMQRGSSFPYTAKSS
  GLGSPTGSFNSTVFGPSSLNSLRQSPMQTIQENRPATFSSVSHYGNQTLQDLLASDSLSHSDVMMTQSDP
  LMSQASTAVSAQNARRNVMLRNDPMMSFAAQPTQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSD
  SSSLGSAKHQQQSPASQSMQTLSDSLSGSSLYSASANLPVMGHDKFPSDLDLDMFNGSLECDMESIIRSE
  LMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019740
• ORF Size: 2016 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019740.3
• RefSeq Size: 2889 bp
• RefSeq ORF: 2019 bp
• Locus ID: 56484
• UniProt ID: Q9WVH4
• Cytogenetics: 10 22.79 cM
• MW: 71.5 kDa
• Gene Summary: Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:18054316, PubMed:18054315, PubMed:23805378). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (PubMed:18054316, PubMed:18054315, PubMed:25402684). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (By similarity). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3' UTR of MYC transcript and prevent its translation (By similarity). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301).[UniProtKB/Swiss-Prot Function]