Bmpr2 (NM_007561) Mouse Tagged ORF Clone

CAT#: MR226598

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Bmpr2 (Myc-DDK-tagged) - Mouse bone morphogenic protein receptor, type II (serine/threonine kinase) (Bmpr2)



  "NM_007561" in other vectors (4)

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USD 1,090.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Bmpr2 (NM_007561) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Bmpr2
Synonyms 2610024H22Rik; AL117858; AW546137; BB189135; BMP-2; BMPR-2; BMPR-II; BMPRII; BRK-3; Gm20272
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR226598 representing NM_007561
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCCTCGCTGCATCGGCCCTTTCGGGTGCCCTGGCTGCTATGGGCCGTCCTGCTGGTCAGCACTA
CGGCTGCTTCTCAGAATCAAGAACGGCTGTGTGCATTTAAAGATCCATATCAACAAGATCTTGGGATAGG
TGAGAGTCGAATCTCTCATGAAAATGGGACAATATTATGTTCCAAAGGGAGCACGTGTTATGGTCTGTGG
GAGAAATCAAAAGGGGACATCAATCTTGTGAAACAAGGATGTTGGTCTCACATCGGTGATCCCCAAGAGT
GCCACTATGAAGAGTGTGTAGTAACTACCACCCCACCCTCAATTCAGAATGGAACGTACCGCTTTTGCTG
CTGTAGTACAGATTTATGTAATGTCAACTTTACTGAGAACTTTCCACCCCCTGACACAACACCACTCAGT
CCACCTCATTCATTTAATCGAGATGAAACGATAATCATTGCTTTGGCATCAGTTTCTGTGTTAGCTGTTT
TGATAGTCGCCTTATGTTTTGGATACAGAATGTTGACAGGAGACCGGAAACAGGGTCTTCACAGCATGAA
CATGATGGAGGCGGCAGCAGCAGAGCCCTCCCTTGACCTGGATAACCTGAAGCTGCTGGAGCTGATTGGA
CGGGGTCGATACGGAGCAGTATATAAAGGTTCCTTGGATGAGCGTCCAGTTGCTGTAAAAGTATTTTCTT
TTGCAAACCGTCAGAATTTTATAAATGAAAAAAACATTTACAGAGTGCCTTTGATGGAACATGACAACAT
TGCTCGCTTCATAGTTGGAGACGAGAGGCTCACTGCAGACGGCCGCATGGAGTATTTGCTTGTGATGGAG
TATTATCCCAATGGATCTCTGTGCAAATATCTGAGTCTCCACACAAGTGATTGGGTAAGCTCTTGCCGTC
TGGCTCATTCTGTGACTAGAGGACTGGCTTATCTTCACACAGAATTACCACGAGGAGATCATTATAAACC
CGCAATCTCCCACCGAGATTTAAACAGCAGGAATGTCCTGGTAAAGAATGACGGCGCGTGTGTTATCAGT
GACTTTGGTTTATCCATGAGGCTAACTGGAAATCGGCTGGTGCGCCCAGGGGAAGAAGATAATGCGGCTA
TAAGTGAGGTTGGCACAATTCGCTATATGGCACCAGAAGTGCTAGAAGGAGCTGTGAACCTGAGGGACTG
TGAGTCAGCTCTGAAGCAAGTGGACATGTATGCGCTTGGACTCATCTACTGGGAGGTGTTTATGAGGTGT
ACAGACCTCTTCCCAGGTGAATCTGTACCAGATTACCAGATGGCTTTTCAGACAGAAGTTGGAAACCATC
CCACATTTGAGGATATGCAGGTTCTTGTGTCCAGAGAGAAGCAGAGACCCAAGTTCCCAGAAGCCTGGAA
AGAAAATAGCCTGGCAGTGAGGTCACTCAAGGAAACAATTGAAGACTGCTGGGACCAGGATGCAGAGGCT
CGGCTCACTGCACAGTGTGCTGAGGAGAGGATGGCTGAACTCATGATGATATGGGAGAGAAACAAGTCTG
TGAGCCCAACGGTCAACCCAATGTCAACTGCTATGCAGAATGAACGCAACCTGTCACATAATAGGCGTGT
GCCAAAAATCGGGCCTTACCCAGATTATTCCTCTTCCTCATATATTGAAGACTCTATCCATCATACTGAC
AGCATTGTGAAGAATATTTCCTCTGAGCATTCGATGTCCAGCACACCATTGACAATAGGAGAAAAGAATC
GAAATTCAATTAATTATGAACGACAGCAAGCACAAGCTCGAATCCCTAGCCCAGAAACAAGCGTCACAAG
CCTGTCCACAAACACAACCACCACAAACACCACCGGCCTCACTCCAAGTACTGGCATGACCACTATATCT
GAGATGCCATACCCAGATGAGACACATTTGCACGCCACAAATGTTGCACAGTCAATCGGGCCAACCCCTG
TCTGCTTACAGCTGACAGAAGAAGACTTGGAGACTAATAAGCTAGATCCAAAAGAAGTTGATAAGAACCT
CAAGGAAAGCTCTGATGAGAATCTCATGGAGCATTCTCTGAAGCAGTTCAGTGGGCCAGACCCATTGAGC
AGTACCAGTTCTAGCTTGCTTTATCCACTCATAAAGCTCGCAGTGGAAGTGACTGGACAACAGGACTTCA
CACAGGCTGCAAATGGGCAAGCATGTTTAATTCCTGATGTTCCACCTGCTCAGATCTATCCTCTCCCTAA
GCAACAGAACCTTCCTAAGAGACCTACTAGTTTGCCTTTGAACACCAAAAATTCAACAAAAGAACCCCGG
CTAAAATTTGGCAACAAGCACAAATCAAACTTGAAACAAGTAGAAACTGGAGTTGCCAAGATGAATACAA
TCAATGCAGCAGAGCCTCATGTGGTGACAGTAACTATGAATGGTGTGGCAGGTAGAAGCCACAATGTTAA
TTCCCATGCTGCCACAACCCAGTATGCCAATGGCGCAGTGCCAGCTGGCCAGGCAGCCAACATAGTGGCA
CATAGGTCCCAAGAAATGCTGCAGAATCAATTTATTGGTGAGGATACCAGGCTGAATATCAATTCCAGTC
CTGATGAGCATGAACCTTTACTGAGACGAGAGCAACAGGCTGGCCATGATGAAGGGGTTCTGGATCGTTT
GGTAGATAGGAGGGAACGGCCATTAGAAGGTGGCCGAACAAATTCCAATAACAACAACAGCAATCCATGT
TCAGAACAAGATATCCTTACACAAGGTGTTACAAGCACAGCTGCAGATCCTGGGCCATCAAAGCCCAGAA
GAGCACAGAGGCCCAATTCTCTGGATCTTTCAGCCACAAATATCCTGGATGGCAGCAGTATACAGATAGG
TGAGTCAACACAAGATGGCAAATCAGGATCAGGTGAAAAGATCAAGAGACGTGTGAAAACTCCATACTCT
CTTAAGCGGTGGCGCCCGTCCACCTGGGTCATCTCCACCGAGCCGCTGGACTGTGAGGTCAACAACAATG
GCAGTGACAGGGCAGTCCATTCTAAATCTAGCACTGCTGTGTACCTTGCAGAGGGAGGCACTGCCACGAC
CACAGTGTCTAAAGATATAGGAATGAATTGTCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226598 representing NM_007561
Red=Cloning site Green=Tags(s)

MTSSLHRPFRVPWLLWAVLLVSTTAASQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLW
EKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDLCNVNFTENFPPPDTTPLS
PPHSFNRDETIIIALASVSVLAVLIVALCFGYRMLTGDRKQGLHSMNMMEAAAAEPSLDLDNLKLLELIG
RGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERLTADGRMEYLLVME
YYPNGSLCKYLSLHTSDWVSSCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGACVIS
DFGLSMRLTGNRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEVFMRC
TDLFPGESVPDYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEA
RLTAQCAEERMAELMMIWERNKSVSPTVNPMSTAMQNERNLSHNRRVPKIGPYPDYSSSSYIEDSIHHTD
SIVKNISSEHSMSSTPLTIGEKNRNSINYERQQAQARIPSPETSVTSLSTNTTTTNTTGLTPSTGMTTIS
EMPYPDETHLHATNVAQSIGPTPVCLQLTEEDLETNKLDPKEVDKNLKESSDENLMEHSLKQFSGPDPLS
STSSSLLYPLIKLAVEVTGQQDFTQAANGQACLIPDVPPAQIYPLPKQQNLPKRPTSLPLNTKNSTKEPR
LKFGNKHKSNLKQVETGVAKMNTINAAEPHVVTVTMNGVAGRSHNVNSHAATTQYANGAVPAGQAANIVA
HRSQEMLQNQFIGEDTRLNINSSPDEHEPLLRREQQAGHDEGVLDRLVDRRERPLEGGRTNSNNNNSNPC
SEQDILTQGVTSTAADPGPSKPRRAQRPNSLDLSATNILDGSSIQIGESTQDGKSGSGEKIKRRVKTPYS
LKRWRPSTWVISTEPLDCEVNNNGSDRAVHSKSSTAVYLAEGGTATTTVSKDIGMNCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_007561
ORF Size 3114 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007561.1, NM_007561.2, NM_007561.3, NM_007561.4, NP_031587.1
RefSeq Size 3507 bp
RefSeq ORF 3117 bp
Locus ID 12168
Cytogenetics 1 C2
MW 115.5 kDa
Gene Summary This gene encodes a serine/threonine kinase that functions as a receptor for bone morphogenetic proteins (BMPs). The encoded protein is a type II receptor that binds extracellular BMPs and forms a complex of two type II and two type I receptors at the cell membrane. This complex signals downstream to activate SMAD transcriptional regulators. This signaling is important during embryonic development. Mutations in this gene can cause pulmonary hypertension. [provided by RefSeq, Mar 2013]

Citations (1)

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