Polg (NM_017462) Mouse Tagged ORF Clone

CAT#: MR226589

  • TrueORF®

Polg (Myc-DDK-tagged) - Mouse polymerase (DNA directed), gamma (Polg)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_017462" in other vectors (4)


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Reconstitution Protocol

USD 1,589.00

In Stock*

Size
    • 10 ug

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Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Polg
Synonyms AA409516; PolgA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226589 representing NM_017462
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGCCTGCTCTGGAAGAAGGTGGCTGGCGCCAAGGTCGCCTCAGGGCCAGTACCAGCAACCGCGC
GCTGGGTCTCCAGCTCCGTCCTCGACCCCGTCCCCAGCGACGGGCGGCCGCCGTCGCAAATGCCCTCCTC
CGAGAATGGGCAGCTGCGGCTCAACCCTCTGCTCATCCAGATGTTGTCGAGAGGCCTGCACGAGCAGATC
TTCGGGTGCGGCGGGGAAATGCCCGACGAGGCCGCGGTGCAGCGCAGCGTAGAGCACCTGCAGAAGCACG
GGCTCTGGGGGCAGCCGGCCACGCCCTTGCCAGACGTGGAGCTGCGCCTGCCCCGGCTCTTCGGGGGCAA
CCTGGACCAGCACTTCCGCCTCCTGGCCCAGAAGCAGAGCCTGCCTTACTTGGAGGCGGCCGCCTCGTTA
TTGGAGGCCCAATTGCCCCCCGAGCCCAAGAGCTGGGCCTGGGCGGAGGGCTGGACCCGGTACGGCCCCG
AGGGGGAGGCCGAACCCGTGGCCATCCCCGAGGAGCGGGCCCTGGTGTTCGACGTGGAGGTCTGCTTGGC
AGAGGGAACCTGCCCCACTTTGGCGGTGGCCATATCCCCCTCGGCCTGGTATTCCTGGTGCAGCCGGCGG
CTGGTGGAAGAGCGTTACTCTTGGACCAGCCAGCTATCGCCGGCTGACCTAATCCCTTTGGGGGGCTCCA
CTAGTGCCAGCAGCTCCACCAAGCAGGATGGGCAGGAACAGTTAGTGGTGGGGCACAATGTTTCCTTTGA
CCGAGCCCATATCAGGGAACAGTATCTGATTCAGGACTCCCGGATGCGTTTTCTCGATACTATGAGCATG
CACATGGCCATCTCGGGGCTGAGCAGCTTCCAGCGCAGCCTGTGGATGGGAGCCAAGCAGGGAAAGCACA
AGACCCAGCAGTCCACAAAGCGAGGGCAGAAGTCCCCGAGGAAAGCCAATGGTCCAGCGATCTCATCTTG
GGACTGGATGGATATCAGCAGTGCCAATAATCTTGCAGATGTGCACAACCTTTATGTGGGGGGACCTCCC
TTAGAGAAGGAGCCTCGGGAGCTGTTCGTCAAAGGCAGCATGAGGGATATCCGAGAGAACTTCCAGGATC
TGATGCAGTACTGCGCCCGTGATGTGTGGGCCACCTTTGAGGTTTTCCAGCAGCAGCTGCCACTCTTCTT
GGAGAGGTGTCCCCACCCAGTGACTCTGGCTGGCATGCTGGAGATGGGTGTGTCCTACCTGCCTGTCAAC
CAGAACTGGGAGCGTTACCTGACAGAGGCACAGAACACATATGAGGAGCTCCAGCGGGAGATGAAGAAGT
CGCTGATGGATCTGGCTAATGATGCCTGTCAGTTGCTCTCAGGAGAGAGGTACAAAGAAGACCCTTGGCT
CTGGGACCTAGAATGGGATTTGCAGGAGTTTAAGCAGAAAAAGGCAAAGAAGGTGAAGAAGCCAGCCTCA
GCCAGCAAGTTGCCCATCGAGGGAGCTGGGCCCTTTGGGGATCCCATGGATCAGGAAGATCCTGGCCCGC
CCAGCGAGGAGGAGGAGCTTCAGCGAAGTGTAACAGCCCACAACCGGTTACAGCAGCTGAGGAGCACCAC
GGACCTCCTGCCTAAGCGACCCCAGCACCTTCCAGGACACCCTGGGTGGTACCGGAAGCTCTGCCCTCGA
CTAGATGACCCTGCCTGGGCTCCCGGCCCCAGCCTCCTCAGCCTGCAAATGCGGGTCACTCCTAAGCTCA
TGGCACTGACCTGGGATGGTTTTCCTCTACACTACTCAGACTCTCATGGTTGGGGCTACCTAGTGCCTGG
GCGTCGGGATAATCTGACCGAGCCGCCAGTGAGCCCCACTGTAGAATCCGCTGCAGTAACCTGCCCCTAC
AGAGCCATCGAGTCCTTATACAGGAAGCACTGCCTTGAACAGGGGAAGCAGCAATTGGAGCCCCAGGAGG
TAGACCTGGCTGAGGAGTTCTTACTCACTGACAGTAGTGCCATGTGGCAAACGGTAGAAGAACTGGGCTG
CTTAGACGTAGAGGCTGAGGCCAAGATGGAGAATTCAGGGCTGAGCCAACCTCTAGTTCTGCCTGCTGCT
TGTGCCCCCAAATCCAGCCAGCCCACGTATCACCATGGCAACGGACCTTATAATGATGTGAATATCCCTG
GTTGCTGGTTTTTCAAGCTGCCTCACAAGGACGGTAACAACTACAATGTGGGCAGTCCCTTTGCCAAAGA
TTTCCTGCCCAAGATGGAGGATGGCACCCTGCAGGCTGGCCCAGGAGGTGCCAGTGGGCCTCGTGCCCTG
GAAATAAATAAAATGATTTCTTTTTGGAGGAATGCTCATAAACGTATCAGTTCCCAGATGGTGGTATGGC
TCCCCAGGTCAGCTCTGCCCCGGGTTGTGACCAGGCACCCCAGCTTCGATGAGGAAGGCCACTATGGGGC
CATCCTACCCCAGGTGGTGACTGCTGGCACCATCACCCGTCGAGCTGTGGAGCCCACGTGGCTCACTGCC
AGTAATGCTCGGCCTGACCGGGTAGGCAGTGAGTTGAAAGCCATGGTGCAGGCTCCACCTGGCTATGTCC
TTGTGGGTGCTGATGTGGACTCACAGGAACTGTGGATCGCAGCTGTACTTGGAGATGCTCACTTTGCTGG
GATGCATGGCTGCACGGCCTTTGGCTGGATGACTCTGCAGGGCAGGAAGAGCAGAGGCACTGATCTGCAC
AGCAAGACAGCTGCCACTGTGGGCATCAGCCGAGAGCATGCCAAAATCTTCAACTATGGCCGCATCTATG
GGGCTGGGCAGTCCTTTGCTGAGCGCCTACTGATGCAGTTCAACCACAGGCTCACAAGGCAGGAGGCAGC
TGAGAAGGCCCAGCAGATGTATGCAGTCACAAAAGGCCTGCGCCGGTATCGGCTGTCTGCGGATGGTGAG
TGGCTGGTGAAACAGTTGAATCTTCCTGTGGACAGGACAGAGGACGGCTGGGTCTCCCTACAAGATCTTC
GAATGATCCGAAGAGAAGCTTCAAGGAAGTCACGATGGAAGAAGTGGGAGGTAGCCTCTGAACGAGCATG
GACAGGGGGCACAGAGTCAGAAATGTTTAATAAGCTGGAGAGTATTGCCATGTCTGATACACCACGTACC
CCAGTACTGGGCTGCTGCATCAGCAGAGCCTTGGAGCCCTCAGTTGTCCAGGGAGAGTTTATAACCAGTC
GTGTGAACTGGGTGGTACAGAGCTCTGCTGTAGACTACTTACATCTCATGCTTGTGGCCATGAAGTGGCT
GTTTGAGGAATTTGCCATTGATGGGCGCTTCTGCATCAGCATCCACGACGAGGTTCGCTACCTGGTGCGT
GAGGAGGACCGCTACCGTGCCGCCCTGGCACTGCAGATCACCAATCTCCTGACCAGGTGCATGTTTGCCT
ATAAGCTGGGTCTGAATGATCTGCCCCAGTCAGTCGCCTTTTTCAGTGCAGTAGACATTGACCAGTGCCT
CAGGAAGGAAGTGACCATGGACTGTAAAACTCCTTCTAACCCAACTGGGATGGAAAGGAGATATGGGATC
CCCCAGGGTGAAGCACTGGATATTTACCAGATAATTGAACTCACCAAAGGCTCCTTGGAAAAACGAAGCC
AGCCTGGACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226589 representing NM_017462
Red=Cloning site Green=Tags(s)

MSRLLWKKVAGAKVASGPVPATARWVSSSVLDPVPSDGRPPSQMPSSENGQLRLNPLLIQMLSRGLHEQI
FGCGGEMPDEAAVQRSVEHLQKHGLWGQPATPLPDVELRLPRLFGGNLDQHFRLLAQKQSLPYLEAAASL
LEAQLPPEPKSWAWAEGWTRYGPEGEAEPVAIPEERALVFDVEVCLAEGTCPTLAVAISPSAWYSWCSRR
LVEERYSWTSQLSPADLIPLGGSTSASSSTKQDGQEQLVVGHNVSFDRAHIREQYLIQDSRMRFLDTMSM
HMAISGLSSFQRSLWMGAKQGKHKTQQSTKRGQKSPRKANGPAISSWDWMDISSANNLADVHNLYVGGPP
LEKEPRELFVKGSMRDIRENFQDLMQYCARDVWATFEVFQQQLPLFLERCPHPVTLAGMLEMGVSYLPVN
QNWERYLTEAQNTYEELQREMKKSLMDLANDACQLLSGERYKEDPWLWDLEWDLQEFKQKKAKKVKKPAS
ASKLPIEGAGPFGDPMDQEDPGPPSEEEELQRSVTAHNRLQQLRSTTDLLPKRPQHLPGHPGWYRKLCPR
LDDPAWAPGPSLLSLQMRVTPKLMALTWDGFPLHYSDSHGWGYLVPGRRDNLTEPPVSPTVESAAVTCPY
RAIESLYRKHCLEQGKQQLEPQEVDLAEEFLLTDSSAMWQTVEELGCLDVEAEAKMENSGLSQPLVLPAA
CAPKSSQPTYHHGNGPYNDVNIPGCWFFKLPHKDGNNYNVGSPFAKDFLPKMEDGTLQAGPGGASGPRAL
EINKMISFWRNAHKRISSQMVVWLPRSALPRVVTRHPSFDEEGHYGAILPQVVTAGTITRRAVEPTWLTA
SNARPDRVGSELKAMVQAPPGYVLVGADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLH
SKTAATVGISREHAKIFNYGRIYGAGQSFAERLLMQFNHRLTRQEAAEKAQQMYAVTKGLRRYRLSADGE
WLVKQLNLPVDRTEDGWVSLQDLRMIRREASRKSRWKKWEVASERAWTGGTESEMFNKLESIAMSDTPRT
PVLGCCISRALEPSVVQGEFITSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVR
EEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDQCLRKEVTMDCKTPSNPTGMERRYGI
PQGEALDIYQIIELTKGSLEKRSQPGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017462
ORF Size 3651 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017462.3
RefSeq Size 4596 bp
RefSeq ORF 3654 bp
Locus ID 18975
Cytogenetics 7 D2
MW 137.1 kDa

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