Ruvbl2 (NM_011304) Mouse Tagged ORF Clone

CAT#: MR226444

Ruvbl2 (Myc-DDK-tagged) - Mouse RuvB-like protein 2 (Ruvbl2)

Product Images

Circular map for MR226444

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ruvbl2
• Synonyms: mp47; p47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCGTGGCAGCCACCACCAAAGTCCCTGAGATCCGAGATGTGACAAGAATCGAGCGAATCGGCG
  CTCACTCCCACATCCGGGGGCTGGGACTGGATGATGCCCTGGAGCCAAGACAGGCTTCCCAGGGAATGGT
  GGGGCAGCTAGCTGCCCGGCGTGCAGCTGGCGTGGTGCTGGAGATGATCCGAGAAGGGAAGATTGCAGGG
  CGGGCCGTCCTCATTGCAGGCCAGCCAGGCACTGGGAAGACAGCCATTGCCATGGGCATGGCCCAGGCAC
  TGGGCCCCGACACACCATTCACAGCCATCGCAGGCAGTGAGATCTTTTCTCTGGAGATGAGCAAGACTGA
  GGCTCTAACGCAAGCCTTCCGCCGTTCCATTGGTGTCCGGATCAAGGAGGAGACAGAGATCATTGAAGGG
  GAGGTGGTAGAGATCCAGATTGACCGGCCAGCCACAGGCACGGGATCCAAGGTGGGCAAACTGACCCTCA
  AGACCACGGAGATGGAGACCATCTACGACCTGGGTACCAAGATGATCGAGTCCCTGACCAAGGACAAGGT
  CCAGGCCGGAGACGTCATCACCATTGACAAGGCCACCGGCAAGATCTCCAAGCTGGGCCGCTCTTTCACA
  CGTGCACGGGACTACGACGCCATGGGCTCCCAGACCAAGTTTGTGCAGTGCCCAGACGGAGAGCTGCAGA
  AACGCAAGGAGGTGGTGCACACCGTGTCCCTCCATGAGATTGACGTCATCAACTCCCGTACTCAGGGCTT
  CCTGGCCCTCTTCTCAGGAGACACAGGGGAGATCAAGTCAGAAGTCCGAGAACAGATCAATGCAAAGGTG
  GCTGAGTGGAGAGAGGAGGGCAAGGCAGAGATCATTCCTGGGGTGCTGTTCATCGACGAGGTCCACATGC
  TGGACATTGAGAGTTTCTCTTTCCTCAACCGGGCCCTGGAGAGCGACATGGCGCCTGTCCTCATCATGGC
  CACCAACCGAGGGATCACCCGGATCCGAGGCACCAGCTACCAGAGTCCCCACGGCATCCCCATCGACCTG
  CTGGACCGCTTGCTCATTGTATCAACGTCACCCTACAGTGAGAAGGATACCAAGCAGATTCTGCGCATCC
  GCTGTGAGGAGGAAGATGTGGAGATGAGTGAGGACGCCTACACAGTGCTGACCCGCATTGGGCTCGAGAC
  CTCCCTGCGCTATGCCATCCAGCTCATCACAGCGGCCAGCCTGGTGTGCCGGAAACGAAAGGGCACCGAG
  GTGCAGGTCGATGACATTAAGCGTGTATACTCCCTCTTCCTGGATGAATCCCGCTCCACACAGTACATGA
  AGGAGTACCAAGATGCCTTCCTCTTCAATGAGCTCAAAGGCGAAACAATGGACACCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATVAATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAG
  RAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEG
  EVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFT
  RARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKV
  AEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDL
  LDRLLIVSTSPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTE
  VQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011304
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011304.3, NP_035434.1
• RefSeq Size: 1675 bp
• RefSeq ORF: 1392 bp
• Locus ID: 20174
• UniProt ID: Q9WTM5
• Cytogenetics: 7 B3
• MW: 51.1 kDa
• Gene Summary: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.[UniProtKB/Swiss-Prot Function]