Apobec3 (NM_001160415) Mouse Tagged ORF Clone

CAT#: MR226390

Apobec3 (Myc-DDK-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3 (Apobec3), transcript variant 1

Product Images

Circular map for MR226390

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apobec3
• Synonyms: Apobec; Arp3; BC003314; Cem15; Rfv3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226390 representing NM_001160415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCATTCTGTCTGGGATGCAGCCATCGCAAATGCTATTCACCGATCAGAAACCTGATATCTCAAG
  AAACATTCAAGTTCCACTTTAAGAACCTAGGCTATGCCAAAGGCCGGAAAGATACCTTCTTGTGCTATGA
  AGTGACTAGAAAGGACTGCGATTCACCCGTCTCCCTTCACCATGGGGTCTTTAAGAACAAGGACAACATC
  CACGCTGAAATCTGCTTTTTATACTGGTTCCATGACAAAGTACTGAAAGTGCTGTCTCCGAGAGAAGAGT
  TCAAGATCACCTGGTATATGTCCTGGAGCCCCTGTTTCGAATGTGCAGAGCAGATAGTAAGGTTCCTGGC
  TACACACCACAACCTGAGCCTGGACATCTTCAGCTCCCGCCTCTACAACGTACAGGACCCAGAAACCCAG
  CAGAATCTTTGCAGGCTGGTTCAGGAAGGAGCCCAGGTGGCTGCCATGGACCTATACGAATTTAAAAAGT
  GTTGGAAGAAGTTTGTGGACAATGGTGGCAGGCGATTCAGGCCTTGGAAAAGACTGCTTACAAATTTTAG
  ATACCAGGATTCTAAGCTTCAGGAGATTCTGAGACCTTGCTACATCTCGGTCCCTTCCAGCTCTTCATCC
  ACTCTGTCAAATATCTGTCTAACAAAAGGTCTCCCAGAGACGAGGTTCTGGGTGGAGGGCAGGCGAATGG
  ACCCGCTAAGTGAAGAGGAATTTTACTCGCAGTTTTACAACCAACGAGTCAAGCATCTCTGCTACTACCA
  CCGCATGAAGCCCTATCTATGCTACCAGCTGGAGCAGTTCAATGGCCAAGCGCCACTCAAAGGCTGCCTG
  CTAAGCGAGAAAGGCAAACAGCATGCAGAAATCCTCTTCCTTGATAAGATTCGGTCCATGGAGCTGAGCC
  AAGTGACAATCACCTGCTACCTCACCTGGAGCCCCTGCCCAAACTGTGCCTGGCAACTGGCGGCATTCAA
  AAGGGATCGTCCAGATCTAATTCTGCATATCTACACCTCCCGCCTGTATTTCCACTGGAAGAGGCCCTTC
  CAGAAGGGGCTGTGTTCTCTGTGGCAATCAGGGATCCTGGTGGACGTCATGGACCTCCCACAGTTTACTG
  ACTGCTGGACAAACTTTGTGAACCCGAAAAGGCCGTTTTGGCCATGGAAAGGATTGGAGATAATCAGCAG
  GCGCACACAAAGGCGGCTCCGCAGGATCAAGGAGTCCTGGGGTCTGCAAGATTTGGTGAATGACTTTGGA
  AACCTACAGCTTGGACCCCCGATGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226390 representing NM_001160415
  Red=Cloning site Green=Tags(s)
  
  MGPFCLGCSHRKCYSPIRNLISQETFKFHFKNLGYAKGRKDTFLCYEVTRKDCDSPVSLHHGVFKNKDNI
  HAEICFLYWFHDKVLKVLSPREEFKITWYMSWSPCFECAEQIVRFLATHHNLSLDIFSSRLYNVQDPETQ
  QNLCRLVQEGAQVAAMDLYEFKKCWKKFVDNGGRRFRPWKRLLTNFRYQDSKLQEILRPCYISVPSSSSS
  TLSNICLTKGLPETRFWVEGRRMDPLSEEEFYSQFYNQRVKHLCYYHRMKPYLCYQLEQFNGQAPLKGCL
  LSEKGKQHAEILFLDKIRSMELSQVTITCYLTWSPCPNCAWQLAAFKRDRPDLILHIYTSRLYFHWKRPF
  QKGLCSLWQSGILVDVMDLPQFTDCWTNFVNPKRPFWPWKGLEIISRRTQRRLRRIKESWGLQDLVNDFG
  NLQLGPPMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160415
• ORF Size: 1287 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160415.1, NP_001153887.1
• RefSeq Size: 2459 bp
• RefSeq ORF: 1290 bp
• Locus ID: 80287
• Cytogenetics: 15 37.85 cM
• MW: 51.5 kDa
• Gene Summary: DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity against HIV-1, simian immunodeficiency viruses (SIVs), mouse mammary tumor virus (MMTV) and friend murine leukemia virus (FrMLV) and may inhibit the mobility of LTR retrotransposons.[UniProtKB/Swiss-Prot Function]