Neo1 (NM_008684) Mouse Tagged ORF Clone

CAT#: MR226235

Neo1 (Myc-DDK-tagged) - Mouse neogenin (Neo1), transcript variant 1

Product Images

Circular map for MR226235

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neo1
• Synonyms: 2610028H22Rik; AI327052; D930014N22Rik; Igdcc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226235 representing NM_008684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGCGCGAAGCCGGGCGACTCCTCTGCACCTCCTCCTCCCGGCGCTGCTGTCCGCCACCGC
  CGCTGCTGCTGTTGCTGCCGCTGCTGCTGCTGCTCGGACGCCCGGCGTCCGGCGCCGCGGCCACGAAGAG
  CGGCTCCCCGCCGCAGTCCGCAGGAGCCAGTGTTCGAACATTCACTCCGTTTTATTTTCTGGTGGAGCCA
  GTAGACACCCTCTCAGTTAGAGGCTCTTCTGTTATATTAAATTGCTCGGCATATTCTGAGCCCTCTCCAA
  ACATTGAATGGAAGAAAGATGGGACTTTTTTAAACTTAGAATCAGATGATCGACGCCAGCTACTCCCAGA
  TGGATCTTTATTCATCAGCAACGTGGTGCATTCCAAACACAATAAGCCTGACGAAGGTTTCTATCAGTGT
  GTAGCCACTGTGGATAATCTTGGAACCATTGTCAGCAGAACAGCCAAGCTCACAGTAGCAGGTCTTCCAA
  GATTTACCAGCCAACCAGAACCTTCTTCAGTCTATGTTGGAAACAGTGCAATTCTGAATTGTGAAGTTAA
  TGCAGATTTGGTCCCATTTGTTAGGTGGGAACAGAATCGACAGCCCCTTCTTCTAGATGACAGGATTGTC
  AAACTTCCAAGTGGAACACTGGTTATCAGCAATGCTACTGAAGGAGATGGGGGACTCTACCGCTGCATTG
  TTGAAAGTGGTGGGCCACCAAAGTTTAGTGACGAAGCTGAATTGAAAGTTCTTCAAGATCCTGAGGAAAT
  TGTAGACTTGGTATTTCTGATGCGACCATCTTCTATGATGAAAGTCACTGGTCAGAGTGCAGTGTTGCCA
  TGTGTTGTCTCAGGGCTTCCTGCTCCAGTTGTTAGATGGATGAAAAACGAAGAAGTGCTTGACACAGAAA
  GCTCTGGCAGGTTGGTCTTGCTAGCAGGAGGTTGCTTGGAGATCAGTGATGTCACTGAGGATGATGCTGG
  GACTTATTTTTGCATAGCTGATAATGGAAATAAGACAGTTGAAGCTCAGGCGGAGCTTACTGTGCAAGTG
  CCACCTGGATTCCTGAAACAACCTGCTAACATATATGCTCACGAATCCATGGACATTGTATTTGAATGTG
  AAGTCACTGGGAAGCCAACTCCAACTGTGAAGTGGGTCAAGAATGGGGATGTGGTTATCCCCAGTGATTA
  CTTTAAAATTGTAAAGGAACATAATCTTCAAGTTTTGGGTCTGGTGAAATCAGATGAAGGGTTCTATCAA
  TGCATTGCTGAGAATGATGTTGGAAATGCACAAGCTGGAGCCCAGCTGATAATCCTTGAGCATGATGTTG
  CCATCCCAACATTACCTCCCACTTCACTGACCAGTGCCACTACTGACCATCTAGCACCAGCCACAACGGG
  ACCATTACCTTCAGCTCCTCGAGACGTCGTGGCCTCCCTGGTCTCTACTCGCTTCATTAAATTGACATGG
  CGTACACCTGCATCAGACCCTCATGGAGACAATCTCACCTACTCTGTGTTCTACACCAAGGAAGGGGTTG
  CTAGGGAGCGTGTTGAGAATACCAGCCAGCCAGGAGAGATGCAGGTGACTATTCAAAACTTGATGCCAGC
  AACTGTGTACATCTTCAAAGTTATGGCTCAAAATAAGCATGGCTCTGGAGAAAGTTCAGCTCCTCTTCGA
  GTAGAGACACAGCCTGAGGTTCAGCTCCCTGGCCCAGCACCTAATATCCGTGCTTATGCAACGTCACCTA
  CTTCTATCACTGTCACCTGGGAAACACCGTTATCTGGCAATGGGGAAATTCAAAATTACAAATTGTACTA
  CATGGAAAAAGGAACTGATAAAGAACAGGATATTGATGTTTCAAGTCACTCCTACACCATTAATGGACTG
  AAGAAATACACAGAATACAGTTTCCGAGTGGTGGCCTACAATAAACATGGTCCTGGAGTTTCTACACAAG
  ATGTTGCTGTTCGAACATTATCAGATGTTCCCAGTGCTGCTCCTCAGAATCTGTCCTTAGAAGTGAGAAA
  TTCAAAGAGTATAGTGATCCACTGGCAGCCCCCTTCCTCAACCACACAAAATGGGCAGATAACTGGCTAC
  AAGATTCGATATCGAAAGGCCTCCCGAAAAAGTGATGTCACTGAGACCTTGGTAACTGGGACACAGCTGT
  CTCAGCTGATTGAAGGTCTTGATCGGGGGACAGAATATAACTTCCGAGTCGCTGCTCTCACAGTCAATGG
  TACAGGTCCAGCAACTGATTGGCTGTCTGCTGAAACTTTTGAAAGCGACCTAGATGAAACTCGTGTTCCT
  GAAGTGCCCAGCTCTCTTCATGTCCGTCCGCTCGTCACTAGCATTGTAGTGAGCTGGACTCCTCCAGAGA
  ACCAGAACATTGTGGTCCGAGGTTATGCCATCGGTTACGGCATTGGCAGCCCTCATGCCCAGACCATCAA
  AGTGGACTATAAACAACGTTATTACACCATCGAAAACTTGGATCCAAGCTCTCATTACGTGATTACCTTG
  AAAGCATTTAACAATGTTGGCGAAGGCATCCCCCTTTATGAGAGTGCTGTGACCAGACCTCACACAGACA
  CTTCTGAAGTTGATTTATTTGTTATTAATGCTCCATACACTCCAGTGCCAGATCCCACTCCCATGATGCC
  ACCAGTGGGAGTTCAGGCTTCCATTCTGAGTCACGACACCATAAGGATTACCTGGGCAGACAACTCCCTG
  CCCAAACACCAGAAGATTACAGACTCCCGCTACTACACAGTCCGGTGGAAGACCAACATCCCAGCAAACA
  CGAAGTACAAGAATGCAAATGCAACGACGTTAAGCTATTTGGTTACTGGTTTAAAGCCAAATACGCTCTA
  TGAGTTCTCTGTGATGGTGACCAAAGGCAGAAGGTCAAGCACGTGGAGTATGACAGCTCATGGCGCTACC
  TTTGAATTAGTTCCTACTTCTCCACCTAAGGATGTGACAGTTGTGAGTAAGGAAGGAAAACCTAGAACCA
  TCATAGTGAATTGGCAGCCTCCCTCTGAAGCTAACGGCAAGATTACAGGTTACATCATCTATTACAGCAC
  GGATGTGAATGCAGAGATACATGACTGGGTTATTGAACCAGTTGTGGGAAACAGACTGACTCACCAGATT
  CAAGAGTTAACACTTGATACGCCATACTACTTCAAAATCCAGGCCCGGAACTCAAAGGGCATGGGGCCCA
  TGTCTGAAGCTGTACAGTTCAGAACACCTAAAGCGGACTCCTCTGATAAAATGCCTAATGACCAAGCCTT
  AGGGTCAGCAGGAAAAGGAAGCCGACTACCAGACCTGGGATCTGACTACAAACCTCCAATGAGTGGCAGC
  AACAGCCCTCACGGGAGCCCCACCTCCCCTCTGGACAGCAACATGCTGCTGGTCATCATTGTCTCTGTTG
  GCGTCATCACTATCGTGGTGGTTGTGGTCATTGCTGTCTTTTGTACCCGGCGCACCACCTCTCACCAGAA
  GAAGAAACGAGCTGCGTGCAAATCAGTGAATGGCTCCCATAAGTACAAGGGCAATTGCAAAGATGTGAAG
  CCTCCAGACCTATGGATCCATCACGAGAGACTAGAGTTGAAGCCTATTGACAAGTCTCCAGATCCTAACC
  CTGTCATGACTGATACTCCAATCCCTCGAAACTCTCAAGATATCACACCAGTGGACAATTCCATGGATAG
  CAATATCCATCAAAGGCGGAATTCATACAGAGGGCATGAGTCAGAGGACAGCATGTCTACACTGGCTGGA
  AGGAGGGGAATGAGACCAAAAATGATGATGCCCTTTGACTCTCAGCCACCTCAGCCTGTGATTAGTGCCC
  ATCCCATCCATTCCCTCGATAACCCTCACCATCATTTCCACTCCAGCAGCCTCGCTTCTCCAGCCCGCAG
  TCATCTCTACCACCCAAGCAGCCCATGGCCCATTGGCACATCCATGTCCCTTTCAGACAGGGCCAATTCC
  ACAGAATCTGTTCGAAATACCCCCAGCACGGACACCATGCCAGCGTCCTCGTCTCAGACGTGCTGCACTG
  ACCATCAGGACCCTGAGGGTGCTACTAGCTCCTCTTACTTGGCCAGCTCCCAAGAGGAAGACTCAGGCCA
  GAGTCTTCCCACAGCCCATGTCCGCCCTTCCCACCCTCTGAAGAGCTTCGCTGTGCCAGCAATCCCACCC
  CCAGGACCTCCTCTCTATGATCCTGCACTGCCAAGCACACCATTACTGTCCCAGCAAGCTCTGAACCATC
  ACATTCACTCAGTGAAAACAGCCTCCATCGGGACGTTAGGAAGGAGCCGGCCTCCTATGCCAGTGGTTGT
  TCCGAGTGCCCCTGAAGTACAGGAGACCACCAGGATGCTGGAAGACTCCGAGAGTAGCTATGAACCAGAT
  GAGCTGACCAAAGAGATGGCCCACCTGGAAGGACTAATGAAGGACCTAAATGCCATCACAACAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226235 representing NM_008684
  Red=Cloning site Green=Tags(s)
  
  MAAEREAGRLLCTSSSRRCCPPPPLLLLLPLLLLLGRPASGAAATKSGSPPQSAGASVRTFTPFYFLVEP
  VDTLSVRGSSVILNCSAYSEPSPNIEWKKDGTFLNLESDDRRQLLPDGSLFISNVVHSKHNKPDEGFYQC
  VATVDNLGTIVSRTAKLTVAGLPRFTSQPEPSSVYVGNSAILNCEVNADLVPFVRWEQNRQPLLLDDRIV
  KLPSGTLVISNATEGDGGLYRCIVESGGPPKFSDEAELKVLQDPEEIVDLVFLMRPSSMMKVTGQSAVLP
  CVVSGLPAPVVRWMKNEEVLDTESSGRLVLLAGGCLEISDVTEDDAGTYFCIADNGNKTVEAQAELTVQV
  PPGFLKQPANIYAHESMDIVFECEVTGKPTPTVKWVKNGDVVIPSDYFKIVKEHNLQVLGLVKSDEGFYQ
  CIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATTGPLPSAPRDVVASLVSTRFIKLTW
  RTPASDPHGDNLTYSVFYTKEGVARERVENTSQPGEMQVTIQNLMPATVYIFKVMAQNKHGSGESSAPLR
  VETQPEVQLPGPAPNIRAYATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDIDVSSHSYTINGL
  KKYTEYSFRVVAYNKHGPGVSTQDVAVRTLSDVPSAAPQNLSLEVRNSKSIVIHWQPPSSTTQNGQITGY
  KIRYRKASRKSDVTETLVTGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVP
  EVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITL
  KAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSL
  PKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGAT
  FELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQI
  QELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMPNDQALGSAGKGSRLPDLGSDYKPPMSGS
  NSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVVIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNCKDVK
  PPDLWIHHERLELKPIDKSPDPNPVMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAG
  RRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPSSPWPIGTSMSLSDRANS
  TESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPP
  PGPPLYDPALPSTPLLSQQALNHHIHSVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPD
  ELTKEMAHLEGLMKDLNAITTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008684
• ORF Size: 4476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008684.2, NP_032710.2
• RefSeq Size: 7388 bp
• RefSeq ORF: 4479 bp
• Locus ID: 18007
• Cytogenetics: 9 B
• MW: 163.4 kDa