Bdh2 (NM_001172055) Mouse Tagged ORF Clone

CAT#: MR226179

Bdh2 (Myc-DDK-tagged) - Mouse 3-hydroxybutyrate dehydrogenase, type 2 (Bdh2), transcript variant 1

Product Images

Circular map for MR226179

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bdh2
• Synonyms: 1810026B04Rik; Dhrs6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226179 representing NM_001172055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTGGATTGTGCCGCAGGATCCACTATGGGCCGACTGGACGGCAAAGTTATTGTCCTGACAGCTG
  CCGCTCAAGGGATTGGACGGGCATCCGCATTAGCTTTTGCAAGAGAAGGAGCCAAAGTCATAGCCACAGA
  TATCAACGAGTCCAAACTCCAGGAGCTGGAAAGTTACCGAGGTATTCAAACTCGAGTCCTTGATGTCACA
  AAGAAGAGACAGATTGATCAGTTTGCCTCAGAAATCGAGAGAATTGATGTTCTCTTTAATGTTGCTGGTT
  TTGTCCACCACGGAACCATCCTGGATTGCGAGGAAAAAGACTGGGACTTCTCAATGAATCTCAACGTCCG
  CAGCATGTTCCTGATGATCAAAGCATTCCTTCCCAAAATGCTTGCTCAGAAGTCAGGCAACATTATCAAC
  ATGTCGTCTGTGGCCTCCAGCATCAAAGGGGTGGAGAACAGATGTGTGTACAGTGCTACCAAGGCGGCTG
  TGATCGGTCTCACCAAGTCCGTGGCTGCAGACTTCATCCAACAGGGCATCAGATGCAACTGTGTGTGTCC
  AGGAACGGTTGACACCCCATCTCTGCAAGAAAGAATACAAGCCAGAGACAATCCCAAAGAGGCACTGAAA
  ACTTTCCTAAACAGACAAAAGACGGGAAGGTTTGCGTCGGCCGAAGAGGTCGCTCTGCTCTGCGTATACC
  TGGCCTCGGACGAGTCAGCCTATGTAACTGGCAACCCTGTCATCATTGATGGGGGCTGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226179 representing NM_001172055
  Red=Cloning site Green=Tags(s)
  
  MSLDCAAGSTMGRLDGKVIVLTAAAQGIGRASALAFAREGAKVIATDINESKLQELESYRGIQTRVLDVT
  KKRQIDQFASEIERIDVLFNVAGFVHHGTILDCEEKDWDFSMNLNVRSMFLMIKAFLPKMLAQKSGNIIN
  MSSVASSIKGVENRCVYSATKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARDNPKEALK
  TFLNRQKTGRFASAEEVALLCVYLASDESAYVTGNPVIIDGGWSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172055
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172055.1, NP_001165526.1
• RefSeq Size: 1172 bp
• RefSeq ORF: 768 bp
• Locus ID: 69772
• Cytogenetics: 3 G3
• MW: 28.1 kDa
• Gene Summary: Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis (PubMed:20550936, PubMed:24863067). Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (PubMed:24863067). Also acts as a 3-hydroxybutyrate dehydrogenase (By similarity).[UniProtKB/Swiss-Prot Function]