Nfatc3 (NM_010901) Mouse Tagged ORF Clone

CAT#: MR226093

Nfatc3 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (Nfatc3)

Product Images

Circular map for MR226093

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfatc3
• Synonyms: C80703; D8Ertd281e; NFAT4; NFATx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226093 representing NM_010901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACTGCAAACTGTGGCGCCCACGACGAGCTCGACTTCAAACTCGTCTTCGGCGAGGACGGGGCAC
  CAGCGCCGGTGTCCCAGGTCTCGCGGCCTGCAGATCTTGAACCAGATGATTGTGCATCCATTTACATCTT
  TAATGTAGATCCACCTCCATCTACTTTAAACTCATCCCTTGGCTTACCACATCATGGACTGCTGCAGTCT
  CACTCTTCTGTTTTGTCACCATCATTTCAGCTCCAAGGTTACAAAAATTATGAAGGAACTGGTGATATTT
  CTGAATCTAAATATAGTCCATTAGGTGGTCCTAAACCCTTTGAGTGCCCAAGTATTCAAATCACATCCAT
  TTCTCCTAACTGTCATCAAGGAACAGATGCTCATGAAGATGACCTACATATAAATGACCCAGAAAGGGAA
  TATTTGGAAAGGCCTTCTAGAGATCATCTCTATCTTCCACTTGAGCCATCCTACCGGGAATCTTCCCTCA
  GTCCTAGTCCTGCCAGCAGCATTTCTTCTAGGAGCTGGTTCTCAGATGCATCTTCTTGTGAATCTCTCTC
  CCACATTTATGATGATGTGGACTCAGAGTTGAATGAAGCTGCTGCACGATTCACTCTTGGTTCACCTCTG
  ACTTCTCCAGGTGGCTCTCCAGGAGGTTGCCCTGGAGAAGAGTCCTGGCATCAACAGTATGGATCTGGAC
  ACTCCTTGTCACCTAGGCAATCTCCTTGCCACTCTCCTAGATCTAGTATCACTGATGAGAATTGGCTGAG
  CCCCAGACCAGCCTCAGGACCCTCATCAAGGCCCACTTCTCCCTGTGGTAAACGACGGCACTCCAGTGCT
  GAAGTATGTTATGCTGGTTCCCTTTCACCCCATCACTCGCCTGTTCCATCCCCTGGTCACTCTCCTAGAG
  GAAGTGTAACAGAAGATACCTGGCTCACTGCTCCTGTCCACACTGGATCAGGCCTCAGCCCTGCACCGTT
  TCCATTTCAGTACTGTGTAGAGACTGACATCCCTTTGAAAACAAGGAAGACTTCTGAAGATCAAGCTGCC
  ATACTACCAGGAAAATTAGAGATCTGTTCAGATGATCAAGGGAACTTATCCCCTTCCCGGGAGACATCAG
  TAGATGATGGCCTTGGATCTCAGTATCCTTTAAAGAAAGATTCATCTGGTGACCAATTTCTTTCAGTTCC
  TTCACCCTTTACCTGGAGCAAACCAAAGCCTGGCCACACCCCTATATTTCGCACATCTTCATTACCTCCA
  TTAGACTGGCCTTTACCAACTCACTTTGGACAATGTGAATTGAAAATAGAAGTGCAACCTAAAACTCACC
  ATAGAGCCCATTATGAAACTGAAGGTAGCCGAGGGGCAGTGAAAGCCTCTACTGGTGGCCATCCTGTTGT
  GAAGCTCCTGGGCTATAGTGAAAAGCCAATAAATCTACAGATGTTTATTGGAACAGCCGATGATAGATAT
  TTACGACCTCATGCATTTTACCAGGTGCATCGGATTACTGGAAAGACAGTTGCTACTGCAAGTCAAGAGA
  TAATAATCGCCAGCACAAAAGTTCTGGAAATCCCACTTCTACCTGAAAATAATATGTCAGCGAGTATTGA
  CTGTGCAGGTATTTTAAAGCTCCGCAACTCAGATATAGAACTTCGAAAAGGAGAAACTGATATTGGCAGA
  AAAAATACTAGAGTCCGACTTGTATTTCGTGTGCACATCCCACAGCCCAGTGGAAAAGTTCTTTCTCTAC
  AGATAGCATCTATTCCTGTTGAGTGCTCTCAGCGATCTGCTCAAGAACTCCCTCATATTGAGAAGTACAG
  TATCAACAGTTGCTCTGTCAATGGAGGCCACGAAATGATTGTGACTGGATCTAATTTTCTTCCAGAATCC
  AAAATAATTTTTCTTGAAAAAGGACAAGATGGAAGACCTCATTGGGAGGCTGAAGGAAAAATAATCAGAG
  AAAAATGTCAAGGGGCTCACATTGTCCTTGAAGTTCCTCCCTATCATAACCCAGCAGTTACATCTGCCGT
  GCAGGTGCACTTTTATCTTTGCAATGGCAAGAGGAAAAAAAGCCAGTCTCAACGTTTTACTTACACACCA
  GTTTTGATGAAGCAAGAACAAAGAGAAGACACAGATTTGCCTTCAGTTCCATCTTTGCCTGTGCCTCATT
  CTGCCCAGGCCCAGAGGCCCTCCTCAGAGACAGGGCACCCCCATGACCGTGCGATGTCAGCACCGGGAAG
  CCTGCTTTGTCAAGTGCAGCCAGCATATACATCTATGGTAGCCTCCACCCATTTGCCACAGTTGCAGTGT
  AGGGATGAAGGTGCTGGTAAAGAGCAGCACATAGCAACATCTTCAGTCATGCACCAGCCTTTCCAAGTTA
  CACCAACATCTCCTATAGGGTCTTCCTATCAGTCTATACAAACTAGTATGTATAACGGTCCAACCTGTCT
  TCCTGTGAATCCTGCCTCTAGTCAAGAATTTGACCCAGTTTTGTTTCAGCAAGATGCAGCTCTTTCTAGT
  TTAGTAAATCTTGGCTGTCAACCACTGTCACCAATACCATTTCATTCTTCAAATTCAGATGCGACAGGAC
  ATCTCTTAGCACATTCACCTCATTCTGTGCAAACCCCACCTCATCTGCAGTCAATGGGATACCATTGTTC
  AAATGCAGGACAAACAGCTCTTTCTTCTCCAGTGGCAGACCAGATCACAGGTCAGCCTTCTTCTCATTTA
  CAGCCTATTACATATTGTCCTTCACACCCAGGATCTGCTACAGCAGCTTCCCCAGCAGCCTCTCATCCTC
  TGGCTAGTTCACCGATTTCTGGGCCATCATCCCCTCAGCTGCAGCCTATGCCTTATCAATCTCCTAGCTC
  AGGAACTGCCTCATCACCATCTCCAACTACCAGAATGCATTCTGGACAGCACTCAACTCAAGCACAAAGT
  ACAGGCCAAGGAGGCCTTTCTGTTCCTTCATCCTTAGTATGTCACAGTTTGTGTGACCCAGCGTCATTTC
  CGCCTGGTGGTGCAACTGTGAGCATTAAACCTGAACCTGAAGATCAAGAACCTAACTTTGCAACCATTGG
  TCTACAGGATATCACCTTAGATGATGTGAACGAGATTATTGGGAGAGACATGTCCCAGATTTCTGTTTCC
  CAAGCCACGGAGGTGATGAGAGACACTCCTCTCCCGGGTCCGGCGTCTCCAGATCTGATGACCTCTCACA
  GTGCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226093 representing NM_010901
  Red=Cloning site Green=Tags(s)
  
  MTTANCGAHDELDFKLVFGEDGAPAPVSQVSRPADLEPDDCASIYIFNVDPPPSTLNSSLGLPHHGLLQS
  HSSVLSPSFQLQGYKNYEGTGDISESKYSPLGGPKPFECPSIQITSISPNCHQGTDAHEDDLHINDPERE
  YLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPL
  TSPGGSPGGCPGEESWHQQYGSGHSLSPRQSPCHSPRSSITDENWLSPRPASGPSSRPTSPCGKRRHSSA
  EVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLTAPVHTGSGLSPAPFPFQYCVETDIPLKTRKTSEDQAA
  ILPGKLEICSDDQGNLSPSRETSVDDGLGSQYPLKKDSSGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPP
  LDWPLPTHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYSEKPINLQMFIGTADDRY
  LRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGR
  KNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMIVTGSNFLPES
  KIIFLEKGQDGRPHWEAEGKIIREKCQGAHIVLEVPPYHNPAVTSAVQVHFYLCNGKRKKSQSQRFTYTP
  VLMKQEQREDTDLPSVPSLPVPHSAQAQRPSSETGHPHDRAMSAPGSLLCQVQPAYTSMVASTHLPQLQC
  RDEGAGKEQHIATSSVMHQPFQVTPTSPIGSSYQSIQTSMYNGPTCLPVNPASSQEFDPVLFQQDAALSS
  LVNLGCQPLSPIPFHSSNSDATGHLLAHSPHSVQTPPHLQSMGYHCSNAGQTALSSPVADQITGQPSSHL
  QPITYCPSHPGSATAASPAASHPLASSPISGPSSPQLQPMPYQSPSSGTASSPSPTTRMHSGQHSTQAQS
  TGQGGLSVPSSLVCHSLCDPASFPPGGATVSIKPEPEDQEPNFATIGLQDITLDDVNEIIGRDMSQISVS
  QATEVMRDTPLPGPASPDLMTSHSAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010901
• ORF Size: 3228 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010901.3
• RefSeq Size: 6032 bp
• RefSeq ORF: 3231 bp
• Locus ID: 18021
• Cytogenetics: 8 53.08 cM
• MW: 116 kDa