Pank1 (NM_001114339) Mouse Tagged ORF Clone

CAT#: MR226077

Pank1 (Myc-DDK-tagged) - Mouse pantothenate kinase 1 (Pank1), transcript variant 1

Product Images

Circular map for MR226077

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pank1
• Synonyms: Pank; Pank1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226077 representing NM_001114339
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGGAGGCGGGCTCCCCGGCCGGGGTCCACCGAGGTTCCCGCAGCTGCGCGCGATGCCGACACGC
  TGCGGGCGAGAGCAGCAAGCCCGGTGCGCGGGGCCCAGCTCGCCGAAGACGTGGGGACGCCGACGGGAGG
  AGGGGAGGAGCGCCGGGGCCACCAGCTCCCGACCGCGGCGCCGAGGCTGCGGGAGAGCAAGCCCCAGGGT
  GGATCGGAAGATCGGGGAACGGCCGACCGTGACCTCCAGCGAGGCTGCCAGAGCCGGAGCCCGCGCACCG
  CTCCGCCGGTCCCGGGCATGGGCGACCGCGGCGCGCAACACGAGCGCGCGGCCCTGCAGTCCCCGGGGGC
  CCCCGAGGGCGCCGCGGCCGCCGTGAACGGGCTGCTGCACAACGGCTTCCACCCGTCGGCCGCCAGCAGC
  CGCGACCCGCCGGCCCCACGCTTCCAGCTCCCGTCCGAGCTGCAGCCGCAGCCGCTGTTCGCCCAGCATG
  ATTCTCCGGCCAAGAAATGCCGGCTGCGGAGGAGGATGGACTCCGGGAGGAAGAACAGGCCGCCATTCCC
  GTGGTTTGGTATGGATATCGGTGGAACCCTGGTTAAGCTGGTTTACTTTGAACCGAAGGATATCACGGCA
  GAGGAAGAACAGGAAGAAGTGGAGAACCTGAAGAGCATCCGGAAGTATTTAACTTCTAACACTGCCTACG
  GCAAAACTGGGATCCGGGACGTCCATCTGGAACTGAAAAACCTGACCATGTGTGGGCGCAAAGGGAACCT
  GCACTTCATCCGCTTCCCGACCTGTGCCATGCACTTGTTCATCCAGATGGGCAGCGAGAAGAACTTCTCC
  AGCCTCCACACCACCCTCTGTGCCACGGGAGGTGGGGCTTTCAAGTTTGAGGAGGACTTCCGAATGATTG
  CGGACCTGCAGCTGCATAAACTGGATGAGCTGGACTGTTTGATTCAGGGCCTTCTTTACGTTGACTCGGT
  TGGCTTCAATGGCAAGCCAGAATGTTACTATTTCGAAAACCCCACAAATCCCGAGTTGTGTCAAAAAAAG
  CCATACTGTTTGGATAACCCGTACCCTATGTTGCTGGTTAACATGGGCTCAGGCGTCAGCATCCTGGCAG
  TGTACTCCAAGGACAACTACAAAAGAGTGACGGGGACCAGTCTTGGAGGTGGGACATTCCTAGGCCTATG
  TTGCTTGCTGACTGGTTGTGAGACCTTTGAAGAAGCTCTGGACATGGCAGCTAAAGGCGACAGCACCAAT
  GTGGATAAGCTGGTGAAGGACATTTACGGAGGAGACTATGAGCGATTTGGCCTTCAAGGATCTGCTGTAG
  CATCAAGCTTTGGCAACATGATGAGCAAAGAAAAGAGAGAGTCCATCAGCAAAGAAGACCTCGCCCGGGC
  CACATTGGTCACCATCACCAACAACATTGGCTCCATTGCTCGGATGTGTGCACTGAATGAGAATATTGAC
  AGAGTTGTGTTTGTTGGGAACTTTCTCAGAATCAATATGGTCTCAATGAAGTTGCTAGCATATGCCATGG
  ACTTTTGGTCTAAAGGACAGCTGAAAGCACTGTTTTTGGAACATGAGGGTTATTTTGGAGCTGTTGGGGC
  CCTGTTGGAACTGTTCAAAATGACCGATACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226077 representing NM_001114339
  Red=Cloning site Green=Tags(s)
  
  MRGRRAPRPGSTEVPAAARDADTLRARAASPVRGAQLAEDVGTPTGGGEERRGHQLPTAAPRLRESKPQG
  GSEDRGTADRDLQRGCQSRSPRTAPPVPGMGDRGAQHERAALQSPGAPEGAAAAVNGLLHNGFHPSAASS
  RDPPAPRFQLPSELQPQPLFAQHDSPAKKCRLRRRMDSGRKNRPPFPWFGMDIGGTLVKLVYFEPKDITA
  EEEQEEVENLKSIRKYLTSNTAYGKTGIRDVHLELKNLTMCGRKGNLHFIRFPTCAMHLFIQMGSEKNFS
  SLHTTLCATGGGAFKFEEDFRMIADLQLHKLDELDCLIQGLLYVDSVGFNGKPECYYFENPTNPELCQKK
  PYCLDNPYPMLLVNMGSGVSILAVYSKDNYKRVTGTSLGGGTFLGLCCLLTGCETFEEALDMAAKGDSTN
  VDKLVKDIYGGDYERFGLQGSAVASSFGNMMSKEKRESISKEDLARATLVTITNNIGSIARMCALNENID
  RVVFVGNFLRINMVSMKLLAYAMDFWSKGQLKALFLEHEGYFGAVGALLELFKMTDTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114339
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114339.2
• RefSeq Size: 3460 bp
• RefSeq ORF: 1647 bp
• Locus ID: 75735
• UniProt ID: Q8K4K6
• Cytogenetics: 19
• MW: 60.5 kDa
• Gene Summary: Plays a role in the physiological regulation of the intracellular CoA concentration.[UniProtKB/Swiss-Prot Function]