Keap1 (NM_001110305) Mouse Tagged ORF Clone

CAT#: MR225867

Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 2

Product Images

Circular map for MR225867

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Keap1
• Synonyms: INRF2; mKIAA0132
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225867 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCGAACCCAAGCTTAGCGGGGCTCCCCGCAGCAGCCAGTTCCTGCCCCTGTGGTCAAAGTGCC
  CCGAGGGGGCCGGGGACGCAGTGATGTATGCCTCCACGGAGTGCAAGGCAGAGGTGACGCCCTCGCAGGA
  CGGTAACCGAACCTTCAGCTACACACTAGAGGATCACACCAAGCAGGCTTTTGGCGTCATGAACGAGCTT
  CGCCTGAGCCAGCAACTCTGTGACGTGACCCTGCAGGTCAAATATGAGGACATCCCAGCTGCCCAATTCA
  TGGCTCACAAAGTGGTGCTGGCCTCCTCCAGCCCAGTCTTTAAAGCCATGTTCACCAACGGGCTTCGGGA
  GCAGGGCATGGAGGTGGTGTCCATCGAAGGCATCCACCCTAAGGTCATGGAAAGGCTTATTGAGTTCGCC
  TACACGGCCTCCATCTCCGTGGGCGAGAAGTGTGTCCTGCACGTGATGAACGGGGCGGTCATGTACCAGA
  TTGACAGCGTGGTTCGAGCCTGCAGCGACTTCCTCGTGCAGCAGCTGGACCCCAGCAACGCCATTGGCAT
  CGCCAACTTCGCGGAGCAGATCGGCTGCACTGAACTGCACCAGCGTGCCCGGGAGTATATCTACATGCAC
  TTCGGGGAGGTGGCCAAGCAGGAGGAGTTCTTCAACCTGTCACACTGCCAGCTGGCCACGCTCATCAGCC
  GGGATGATCTGAACGTACGCTGCGAGTCCGAGGTGTTCCACGCGTGCATCGACTGGGTCAAATACGACTG
  CCCGCAGCGGCGCTTCTACGTGCAGGCACTGCTGCGGGCCGTGCGCTGCCATGCGCTCACGCCGCGCTTC
  CTGCAGACGCAGCTGCAGAAGTGTGAGATCCTGCAGGCCGACGCGCGCTGCAAGGACTACCTGGTGCAGA
  TATTCCAGGAGCTCACGCTGCACAAGCCCACGCAGGCAGTGCCCTGCCGCGCGCCCAAAGTGGGCCGCCT
  CATCTACACAGCGGGCGGTTACTTCCGACAGTCGCTCAGCTACCTGGAGGCCTACAACCCGAGCAATGGC
  TCCTGGCTGCGCCTGGCCGATCTACAGGTGCCGCGCAGTGGGCTGGCAGGCTGCGTGGTGGGTGGGCTGC
  TATACGCTGTGGGCGGCCGCAACAACTCTCCGGATGGCAACACTGACTCCAGCGCCCTGGACTGCTACAA
  CCCCATGACCAACCAGTGGTCGCCCTGTGCCTCTATGAGCGTGCCACGCAACCGCATCGGGGTGGGGGTC
  ATAGATGGCCACATCTACGCAGTCGGGGGTTCCCACGGCTGCATCCACCACAGCAGCGTGGAGAGATATG
  AGCCAGAGCGGGACGAGTGGCATCTAGTCGCGCCAATGTTGACACGGAGGATTGGCGTGGGCGTGGCAGT
  GCTCAACCGCTTGCTGTATGCAGTGGGGGGCTTTGACGGGACTAACCGGCTTAACTCCGCAGAATGTTAC
  TATCCAGAGAGGAATGAGTGGCGGATGATCACACCGATGAATACCATCCGGAGCGGGGCCGGGGTCTGCG
  TGCTGCACAACTGTATCTATGCAGCAGGGGGCTACGATGGGCAGGACCAGTTGAACAGTGTGGAGCGCTA
  CGACGTGGAGACAGAGACCTGGACTTTCGTAGCCCCCATGAGGCATCACCGTAGTGCGCTGGGGATTACT
  GTGCACCAGGGCAAGATCTACGTCCTCGGAGGCTATGATGGCCACACTTTTCTGGACAGTGTGGAATGCT
  ATGACCCGGACAGTGATACCTGGAGTGAGGTGACCCGCATGACATCTGGCCGCAGCGGGGTGGGTGTGGC
  CGTCACCATGGAACCCTGTCGGAAGCAAATTGATCAACAAAACTGTACCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225867 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTPSQDGNRTFSYTLEDHTKQAFGVMNEL
  RLSQQLCDVTLQVKYEDIPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFA
  YTASISVGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMH
  FGEVAKQEEFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHALTPRF
  LQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSNG
  SWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCASMSVPRNRIGVGV
  IDGHIYAVGGSHGCIHHSSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECY
  YPERNEWRMITPMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRHHRSALGIT
  VHQGKIYVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110305
• ORF Size: 1872 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110305.1, NP_001103775.1
• RefSeq Size: 3434 bp
• RefSeq ORF: 1875 bp
• Locus ID: 50868
• UniProt ID: Q9Z2X8
• Cytogenetics: 9 A3
• MW: 69.6 kDa
• Gene Summary: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[UniProtKB/Swiss-Prot Function]