Wt1 (NM_144783) Mouse Tagged ORF Clone

CAT#: MR225851

Wt1 (Myc-DDK-tagged) - Mouse Wilms tumor 1 homolog (Wt1)

Product Images

Circular map for MR225851

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wt1
• Synonyms: D630046I19Rik; Wt-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225851 representing NM_144783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGACTTCCTCCTGTCGCAGGAGCCGGCTTCCACGTGTGTCCCGGAGCCGGCGTCTCAGCACACGCTCC
  GCCGGGAGCCCGGGTGCGTCCAGCAGCCGGAGCAACCTGGGGACCGAGGCCCCCGGAGCGCCTGGGCCAA
  GTCCAGCGCCGAGAATCCGCAGGATCGCAGGAGCGGAGAACCGTCCGCATCCGAGCCGCACCTCATGGGT
  TCCGACGTGCGGGACCTGAACGCGCTGCTGCCCGCTGTGTCTTCGCTGGGCGGCGGCGGCGGCGGCTGCG
  GGCTCCCTGTGAGCGGCGCAGCGCAGTGGGCGCCCGTGTTGGACTTCGCGCCTCCGGGCGCCTCGGCTTA
  CGGGTCGCTGGGCGGTCCCGCGCCTCCTCCCGCTCCGCCGCCGCCTCCGCCGCCACCCCACTCCTTCATC
  AAACAGGAGCCCAGCTGGGGCGGCGCCGAGCCACACGAGGAGCAGTGCCTGAGCGCCTTCACCTTGCACT
  TCTCGGGCCAGTTCACCGGTACAGCCGGGGCCTGTCGCTACGGACCCTTCGGTCCTCCCCCGCCCAGCCA
  GGCGTCCTCGGGCCAGGCCAGGATGTTCCCCAATGCGCCCTACCTGCCCAGCTGCCTGGAGAGCCAGCCT
  ACCATCCGCAACCAAGGATACAGCACGGTCACTTTCGACGGGGCGCCCAGCTATGGCCACACGCCCTCGC
  ATCACGCGGCGCAGTTCCCCAACCATTCCTTCAAACACGAGGACCCCATGGGCCAGCAGGGCTCGCTGGG
  CGAGCAGCAGTACTCCGTGCCACCTCCGGTGTATGGCTGCCACACCCCTACTGACAGTTGCACAGGCAGC
  CAGGCCCTGCTCCTGAGGACGCCCTACAGCAGTGACAATTTATACCAAATGACCTCCCAGCTTGAATGCA
  TGACCTGGAATCAGATGAACCTAGGAGCTACCTTAAAGGGAATGGCTGCTGGGAGCTCCAGCTCAGTGAA
  ATGGACAGAAGGGCAGAGCAACCACGGCACAGGGTATGAGAGTGAGAACCACACGGCCCCCATCCTCTGT
  GGTGCCCAGTACAGAATACACACCCACGGGGTCTTCCGAGGCATTCAGGATGTGCGGCGTGTATCTGGAG
  TGGCCCCAACTCTTGTCCGGTCAGCATCTGAAACCAGTGAGAAACGTCCTTTCATGTGTGCATACCCAGG
  CTGCAATAAGAGATATTTTAAGCTGTCCCACTTACAGATGCATAGCCGGAAGCACACTGGTGAGAAACCA
  TACCAGTGTGACTTCAAGGACTGCGAGAGAAGGTTTTCTCGCTCAGACCAGCTCAAAAGACACCAAAGGA
  GACACACAGGTGTGAAACCATTCCAGTGTAAAACTTGTCAGCGAAAGTTTTCCCGGTCCGACCATCTGAA
  GACCCACACCAGGACTCATACAGGTAAAACAAGTGAAAAGCCCTTCAGCTGTCGGTGGCACAGTTGTCAG
  AAAAAGTTTGCGCGCTCAGACGAATTGGTCCGCCATCACAACATGCATCAGAGAAACATGACCAAACTCC
  AGCTGGCGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225851 representing NM_144783
  Red=Cloning site Green=Tags(s)
  
  LDFLLSQEPASTCVPEPASQHTLRREPGCVQQPEQPGDRGPRSAWAKSSAENPQDRRSGEPSASEPHLMG
  SDVRDLNALLPAVSSLGGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFI
  KQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQP
  TIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGS
  QALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTAPILC
  GAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP
  YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQ
  KKFARSDELVRHHNMHQRNMTKLQLAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144783
• ORF Size: 1646 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144783.2, NP_659032.3
• RefSeq Size: 3092 bp
• RefSeq ORF: 1554 bp
• Locus ID: 22431
• Cytogenetics: 2 55.06 cM
• MW: 56.5 kDa
• Gene Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013]