Il18r1 (NM_001161842) Mouse Tagged ORF Clone

CAT#: MR225770

Il18r1 (Myc-DDK-tagged) - Mouse interleukin 18 receptor 1 (Il18r1), transcript variant 2

Product Images

Circular map for MR225770

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il18r1
• Synonyms: Il1rrp; Il18ralpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225770 representing NM_001161842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCATGAAGAATTAATCTTGACACTCTGCATTCTCATTGTTAAAAGTGCCTCAAAAAGTTGTATTC
  ACCGATCACAAATTCATGTGGTAGAGGGAGAACCTTTTTATCTGAAGCCATGTGGCATATCTGCACCAGT
  GCACAGGAATGAAACAGCCACCATGAGATGGTTCAAAGGCAGTGCTTCACATGAGTATAGAGAGCTGAAC
  AACAGAAGCTCGCCCAGAGTCACTTTTCATGATCACACCTTGGAATTCTGGCCAGTTGAGATGGAGGATG
  AGGGAACGTACATTTCTCAAGTCGGAAATGATCGTCGCAATTGGACCTTAAATGTCACCAAAAGAAACAA
  ACACAGCTGTTTCTCTGACAAGCTCGTGACAAGCAGAGATGTTGAAGTTAACAAATCTCTGCATATCACT
  TGTAAGAATCCTAACTATGAAGAGCTGATCCAGGACACATGGCTGTATAAGAACTGTAAGGAAATATCCA
  AAACCCCAAGGATCCTGAAGGATGCCGAGTTTGGAGATGAGGGCTACTACTCCTGCGTGTTTTCTGTCCA
  CCATAATGGGACACGGTACAACATCACCAAGACTGTCAATATAACAGTTATTGAAGGAAGGAGTAAAGTA
  ACTCCAGCTATTTTAGGACCAAAGTGTGAGAAGGTTGGTGTAGAACTAGGAAAGGATGTGGAGTTGAACT
  GCAGTGCTTCATTGAATAAAGACGATCTGTTTTATTGGAGCATCAGGAAAGAGGACAGCTCAGACCCTAA
  TGTGCAAGAAGACAGGAAGGAGACGACAACATGGATTTCTGAAGGCAAACTGCATGCTTCAAAAATACTG
  AGATTTCAGAAAATTACTGAAAACTATCTCAATGTTTTATATAATTGCACCGTGGCCAACGAAGAAGCCA
  TAGACACCAAGAGCTTCGTCTTGGTGAGAAAAGAAATACCTGATATCCCAGGCCATGTCTTTACAGGAGG
  AGTAACTGTGCTTGTTCTCGCCTCTGTGGCAGCAGTGTGTATAGTGATTTTGTGTGTCATTTATAAAGTT
  GACTTGGTTCTGTTCTATAGGCGCATAGCGGAAAGAGACGAGACACTAACAGGTAATATGCTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225770 representing NM_001161842
  Red=Cloning site Green=Tags(s)
  
  MHHEELILTLCILIVKSASKSCIHRSQIHVVEGEPFYLKPCGISAPVHRNETATMRWFKGSASHEYRELN
  NRSSPRVTFHDHTLEFWPVEMEDEGTYISQVGNDRRNWTLNVTKRNKHSCFSDKLVTSRDVEVNKSLHIT
  CKNPNYEELIQDTWLYKNCKEISKTPRILKDAEFGDEGYYSCVFSVHHNGTRYNITKTVNITVIEGRSKV
  TPAILGPKCEKVGVELGKDVELNCSASLNKDDLFYWSIRKEDSSDPNVQEDRKETTTWISEGKLHASKIL
  RFQKITENYLNVLYNCTVANEEAIDTKSFVLVRKEIPDIPGHVFTGGVTVLVLASVAAVCIVILCVIYKV
  DLVLFYRRIAERDETLTGNMLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161842
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161842.1, NP_001155314.1
• RefSeq Size: 1919 bp
• RefSeq ORF: 1119 bp
• Locus ID: 16182
• Cytogenetics: 1 B
• MW: 43 kDa
• Gene Summary: Within the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (By similarity). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (PubMed:26099046).[UniProtKB/Swiss-Prot Function]