Pard3 (NM_001122850) Mouse Tagged ORF Clone

CAT#: MR225741

Pard3 (Myc-DDK-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 4

Product Images

Circular map for MR225741

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pard3
• Synonyms: AA960621; AI256638; Asip; D8Ertd580e; Par-3; Par3; Pard-3; Pard3a; Phip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225741 representing NM_001122850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGACCGTGTGCTTCGGGAGGACCCGGGTGGTCGTGCCGTGCGGAGATGGCCGCATGAAAGTTT
  TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCGTGGCCAAGGATCCAAACTACTGGATACA
  GGTGCATCGCTTGGAGCATGGAGATGGAGGGATTCTAGACCTGGATGACATCCTCTGTGACGTTGCTGAT
  GACAAAGACAGACTGGTAGCAGTATTTGATGAACAGGATCCCCACCATGGAGGAGATGGTACCAGCGCCA
  GCTCCACGGGAACCCAGAGTCCAGAGATATTCGGCAGTGAGCTGGGCACCAACAATGTTTCTGCTTTTCA
  GCCTTATCAAGCCACAAGTGAAATTGAGGTCACGCCTTCAGTTCTTCGGGCAAATATGCCTCTTCATGTC
  CGCCGGAGCAGCGACCCAGCTTTAACTGGCCTTTCCACTTCTGTCAGTGATAACAACTTTTCCTCAGAGG
  AGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCAGAACACCGCTGGAAG
  TCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTTCCACGGGATCCCAGTAGCTGGTCC
  AACCAGTTCCAGCGAGACAACGCCCGCTCCTCCCTGAGCGCCAGCCACCCAATGGTAGACCGGTGGCTGG
  AGAAGCAAGAACAGGATGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCCGGTTGGACATGCTGA
  TACCGGATTGGAGAACATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTACAAGTCCCCAACGAT
  GGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACATTGGGGTTGTTAGTGA
  AGCGGTTGGAGAAAGGCGGTAAGGCTGAGCAAGAAAACCTTTTCCATGAGAATGACTGCATTGTGAGGAT
  TAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAACATATGTTCCGCCAAGCTATGCGTGCG
  CGTGTCATTTGGTTCCATGTGGTCCCTGCAGCAAACAAGGAGCAATATGAACAACTGTCCCAACGCGAGA
  AGAACAACTACTCCCCAGGCCGCTTCAGCCCTGACAGCCACTGTGTGGCCAACAGGAGTGTGGCCAACAA
  TGCCCCTCAAGCATTGCCCAGAGCACCCAGACTGAGTCAGCCACCCGAGCAGCTGGATGCTCACCCCCGA
  CTACCTCATAGTGCTCACGCCTCAACCAAACCACCCGCAGCCCCGGCCTTGGCTCCACCCAGTGTGCTTA
  GTACCAACGTAGGCAGTGTGTACAACACGAAGAAAGTAGGCAAGAGGCTCAACATCCAGCTTAAGAAAGG
  TACAGAAGGACTGGGATTCAGCATCACCTCCCGGGACGTCACCATAGGTGGCTCAGCTCCCATTTATGTC
  AAGAATATCCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGACTCAAGGCAGGAGACCGGCTAATAGAGG
  TCAATGGAGTAGATTTAGCAGGCAAATCCCAGGAGGAAGTTGTTTCCCTGTTGAGAAGCACCAAGATGGA
  GGGGACTGTGAGCCTTCTGGTCTTTCGTCAGGAAGAGGCTTTCCACCCAAGGGAAATGAATGCTGAACCA
  AGCCAGATGCAGACTCCAAAAGAAACGAAAGCTGAAGATGAGGACGTTGTTCTCACACCCGATGGTACCA
  GGGAGTTTCTGACTTTTGAAGTTCCACTGAATGACTCAGGATCTGCAGGGCTTGGTGTCAGTGTCAAGGG
  GAACCGTTCCAAAGAGAACCACGCAGATTTGGGGATCTTCGTTAAATCCATTATCAATGGTGGAGCTGCA
  TCTAAAGATGGAAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAATGGAGAATCTCTACTGGGCAAAG
  CCAACCAGGAAGCCATGGAGACTCTACGGAGGTCCATGTCCACCGAGGGCAACAAGCGTGGCATGATCCA
  GCTCATTGTGGCGAGGCGGATCAGCAGATGTAACGAGCTGCGGTCTCCTGGGAGCCCTGCTGCACCTGAG
  CTGCCCATCGAGACAGAACTGGATGACCGAGAACGCAGGATCTCACACTCCCTCTACAGTGGGATCGAGG
  GGCTGGATGAGTCGCCCACCAGGAACGCAGCACTGAGCAGGATAATGGGAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225741 representing NM_001122850
  Red=Cloning site Green=Tags(s)
  
  MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVAD
  DKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHV
  RRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDPSSWS
  NQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPND
  GGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRA
  RVIWFHVVPAANKEQYEQLSQREKNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPR
  LPHSAHASTKPPAAPALAPPSVLSTNVGSVYNTKKVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYV
  KNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEP
  SQMQTPKETKAEDEDVVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAA
  SKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPE
  LPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122850
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122850.2
• RefSeq Size: 4178 bp
• RefSeq ORF: 2226 bp
• Locus ID: 93742
• Cytogenetics: 8 74.66 cM
• MW: 81.8 kDa
• Gene Summary: Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390).[UniProtKB/Swiss-Prot Function]