Adrb3 (NM_013462) Mouse Tagged ORF Clone

CAT#: MR225687

Adrb3 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3)

Product Images

Circular map for MR225687

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adrb3
• Synonyms: Adrb-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225687 representing NM_013462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCGTGGCCTCACAGAAACGGCTCTCTGGCTTTGTGGTCGGACGCCCCTACCCTGGACCCCAGTG
  CAGCCAACACCAGTGGGTTGCCAGGAGTACCATGGGCAGCGGCATTGGCTGGGGCATTGCTGGCGCTGGC
  CACGGTGGGAGGCAACCTGCTGGTAATCATAGCCATCGCCCGCACGCCGAGACTACAGACCATAACCAAC
  GTGTTCGTGACTTCACTGGCCGCAGCTGACTTGGTAGTGGGACTCCTCGTAATGCCACCAGGGGCCACAT
  TGGCGCTGACTGGCCATTGGCCCTTGGGCGAAACTGGTTGCGAACTGTGGACGTCAGTGGACGTGCTCTG
  TGTAACTGCTAGCATCGAGACCTTGTGCGCCCTGGCTGTGGACCGCTACCTAGCTGTCACCAACCCTTTG
  CGTTACGGCACGCTGGTTACCAAGCGCCGCGCCCGCGCGGCAGTTGTCCTGGTGTGGATCGTGTCCGCTG
  CCGTGTCCTTTGCGCCCATCATGAGCCAGTGGTGGCGTGTAGGGGCAGATGCCGAGGCACAGGAATGCCA
  CTCCAATCCGCGCTGCTGTTCCTTTGCCTCCAACATGCCCTATGCGCTGCTCTCCTCCTCCGTCTCCTTC
  TACCTTCCCCTCCTTGTGATGCTCTTCGTCTATGCTCGAGTGTTCGTTGTGGCTAAGCGCCAACGGCATT
  TGCTGCGCCGGGAACTGGGCCGCTTCTCGCCCGAGGAGTCTCCGCCGTCTCCGTCGCGCTCTCCGTCCCC
  TGCCACAGGCGGGACACCCGCGGCACCGGATGGAGTGCCCCCCTGCGGCCGGCGGCCTGCGCGCCTCCTG
  CCACTCCGGGAACACCGCGCCCTGCGCACCTTAGGTCTCATTATGGGCATCTTCTCTCTGTGCTGGCTGC
  CCTTCTTCCTGGCCAACGTGCTGCGCGCACTCGCGGGGCCCTCTCTAGTTCCCAGCGGAGTTTTCATCGC
  CCTGAACTGGCTGGGCTATGCCAACTCCGCCTTCAACCCGGTCATCTACTGCCGCAGCCCGGACTTTCGC
  GACGCCTTCCGTCGTCTTCTGTGTAGCTACGGTGGCCGTGGACCGGAGGAGCCACGCGCAGTCACCTTCC
  CAGCCAGCCCTGTTGAAGCCAGGCAGAGTCCACCGCTCAACAGGTTTGATGGCTATGAAGGTGCGCGTCC
  GTTTCCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225687 representing NM_013462
  Red=Cloning site Green=Tags(s)
  
  MAPWPHRNGSLALWSDAPTLDPSAANTSGLPGVPWAAALAGALLALATVGGNLLVIIAIARTPRLQTITN
  VFVTSLAAADLVVGLLVMPPGATLALTGHWPLGETGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPL
  RYGTLVTKRRARAAVVLVWIVSAAVSFAPIMSQWWRVGADAEAQECHSNPRCCSFASNMPYALLSSSVSF
  YLPLLVMLFVYARVFVVAKRQRHLLRRELGRFSPEESPPSPSRSPSPATGGTPAAPDGVPPCGRRPARLL
  PLREHRALRTLGLIMGIFSLCWLPFFLANVLRALAGPSLVPSGVFIALNWLGYANSAFNPVIYCRSPDFR
  DAFRRLLCSYGGRGPEEPRAVTFPASPVEARQSPPLNRFDGYEGARPFPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013462
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013462.3, NP_038490.2
• RefSeq Size: 2795 bp
• RefSeq ORF: 1203 bp
• Locus ID: 11556
• UniProt ID: P25962
• Cytogenetics: 8 15.94 cM
• MW: 43.5 kDa
• Gene Summary: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.[UniProtKB/Swiss-Prot Function]