Fzd3 (NM_021458) Mouse Tagged ORF Clone

CAT#: MR225501

Fzd3 (Myc-DDK-tagged) - Mouse frizzled homolog 3 (Drosophila) (Fzd3)

Product Images

Circular map for MR225501

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fzd3
• Synonyms: AU020229; D930050A07Rik; Fz3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225501 representing NM_021458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGAGCTGGATTGTCTTTGATCTTTGGCTCTTGACTGTGTTTCTGGGGCAGATAGGTGGGCACA
  GTTTGTTTTCTTGTGAACCTATAACCTTGAGGATGTGCCAAGATTTGCCTTACAATACTACCTTCATGCC
  TAATCTTCTGAACCATTATGACCAACAGACTGCAGCTTTAGCAATGGAGCCCTTCCACCCTATGGTGAAC
  CTGGATTGTTCTCGGGATTTTCGGCCATTTCTTTGTGCACTCTATGCCCCTATTTGTATGGAATATGGAC
  GTGTCACACTTCCCTGCCGTAGGCTGTGTCAGCGTGCCTATAGCGAGTGTTCAAAACTCATGGAGATGTT
  TGGTGTCCCGTGGCCTGAAGATATGGAGTGCAGTAGGTTTCCAGATTGTGATGAGCCATATCCCCGACTT
  GTGGATTTGAATTTAGTTGGAGATCCAACTGAAGGAGCCCCAGTTGCAGTGCAGAGGGACTATGGTTTTT
  GGTGTCCCAGAGAGTTAAAAATTGATCCTGATCTTGGCTATTCCTTTCTGCACGTGCGAGATTGTTCGCC
  ACCATGTCCCAATATGTACTTCAGGAGAGAAGAACTGTCATTTGCTCGCTATTTCATAGGCCTGATTTCA
  ATCATTTGCCTCTCTGCCACATTGTTTACTTTTTTAACCTTTCTAATTGACGTCACAAGATTCCGTTACC
  CTGAAAGACCTATCATATTTTATGCAGTCTGCTACATGATGGTGTCATTAATTTTCTTCATTGGGTTTTT
  GCTGGAGGACCGAGTAGCCTGCAATGCATCTAGCCCTGCACAGTATAAGGCTTCTACAGTGACACAAGGA
  TCTCACAATAAGGCCTGTACCATGCTCTTTATGGTACTATATTTTTTCACTATGGCTGGCAGTGTATGGT
  GGGTAATTCTTACCATCACATGGTTTTTAGCAGCTGTGCCAAAGTGGGGCAGTGAAGCTATTGAGAAGAA
  AGCATTGCTGTTTCATGCCAGTGCCTGGGGCATCCCCGGAACTCTAACTATCATCCTTTTAGCGATGAAT
  AAAATTGAAGGTGACAATATTAGTGGCGTGTGTTTTGTCGGCCTCTACGACGTTGATGCATTAAGATATT
  TCGTTCTCGCTCCCCTCTGCCTGTATGTGGTAGTTGGGGTTTCTCTCCTTTTAGCCGGCATTATATCCCT
  AAACAGAGTTCGGATTGAGATCCCATTAGAAAAGGAAAACCAAGATAAGTTAGTGAAGTTCATGATCCGG
  ATTGGTGTTTTCAGCATTCTCTACCTTGTGCCACTCTTGGTTGTAATTGGATGTTACTTTTATGAGCAAG
  CTTACCGCGGCATCTGGGAGACAACATGGATCCAGGAACGCTGCAGAGAGTATCACATTCCATGTCCGTA
  CCAGGTTACTCAGATGAGTCGTCCAGACCTGATTCTCTTTCTGATGAAGTATCTCATGGCTCTCATAGTT
  GGGATTCCCTCTATATTTTGGGTTGGAAGCAAAAAGACATGCTTTGAATGGGCCAGTTTTTTCCATGGGC
  GTAGGAAAAAAGAGATAGTGAATGAGAGCCGGCAGGTGCTCCAGGAACCTGACTTTGCTCAGTCACTCCT
  GAGGGACCCAAATACTCCAATTATAAGAAAATCAAGAGGAACTTCCACTCAAGGGACATCCACACATGCT
  TCTTCAACTCAGCTGGCCATGGTGGATGACCAAAGAAGCAAAGCAGGGAGTGTCCACAGCAAAGTGAGCA
  GCTACCATGGCAGCCTCCACAGGTCACGGGATGGCAGGTACACTCCCTGCAGTTACCGAGGAATGGAGGA
  GAGACTACCTCACGGCAGCATGTCACGGCTGACGGATCATTCCAGGCACAGTAGTTCTCATCGGCTCAAC
  GAGCAGTCCCGACACAGCAGCATCCGAGACCTCAGTAACAACCCCATGACTCACATTACACATGGCACCA
  GCATGAACCGTGTTATTGAGGAGGATGGAACCAGTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225501 representing NM_021458
  Red=Cloning site Green=Tags(s)
  
  MAVSWIVFDLWLLTVFLGQIGGHSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHPMVN
  LDCSRDFRPFLCALYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDMECSRFPDCDEPYPRL
  VDLNLVGDPTEGAPVAVQRDYGFWCPRELKIDPDLGYSFLHVRDCSPPCPNMYFRREELSFARYFIGLIS
  IICLSATLFTFLTFLIDVTRFRYPERPIIFYAVCYMMVSLIFFIGFLLEDRVACNASSPAQYKASTVTQG
  SHNKACTMLFMVLYFFTMAGSVWWVILTITWFLAAVPKWGSEAIEKKALLFHASAWGIPGTLTIILLAMN
  KIEGDNISGVCFVGLYDVDALRYFVLAPLCLYVVVGVSLLLAGIISLNRVRIEIPLEKENQDKLVKFMIR
  IGVFSILYLVPLLVVIGCYFYEQAYRGIWETTWIQERCREYHIPCPYQVTQMSRPDLILFLMKYLMALIV
  GIPSIFWVGSKKTCFEWASFFHGRRKKEIVNESRQVLQEPDFAQSLLRDPNTPIIRKSRGTSTQGTSTHA
  SSTQLAMVDDQRSKAGSVHSKVSSYHGSLHRSRDGRYTPCSYRGMEERLPHGSMSRLTDHSRHSSSHRLN
  EQSRHSSIRDLSNNPMTHITHGTSMNRVIEEDGTSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021458
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021458.2, NP_067433.1
• RefSeq Size: 12742 bp
• RefSeq ORF: 2001 bp
• Locus ID: 14365
• UniProt ID: Q61086
• Cytogenetics: 14 34.09 cM
• MW: 76.7 kDa
• Gene Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Required for the development of major fiber tracts in the central nervous system, including: the anterior commissure, the corpus callosum, the thalamocortical, corticothalamic and nigrostriatal tracts, the corticospinal tract, the fasciculus retroflexus, the mammillothalamic tract, the medial lemniscus, and ascending fiber tracts from the spinal cord to the brain. In the peripheral nervous system, controls axon growth in distinct populations of cranial and spinal motor neurons, including the facial branchimotor nerve, the hypoglossal nerve, the phrenic nerve, and motor nerves innervating dorsal limbs. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner.[UniProtKB/Swiss-Prot Function]