Syn2 (NM_013681) Mouse Tagged ORF Clone

CAT#: MR225315

Syn2 (Myc-DDK-tagged) - Mouse synapsin II (Syn2), transcript variant IIb

Product Images

Circular map for MR225315

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syn2
• Synonyms: 2900074L19Rik; AI836018; AI841723
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225315 representing NM_013681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAACTTCCTGAGGCGCCGGCTGTCGGACAGCAGCTTCATCGCCAACCTGCCCAATGGCTACATGA
  CTGACCTTCAGCGCCCGGAGCCGCAGCAGCCCCCTCCTGCCCCTGGCCCCGGTGCCGCTACGGCGTCGGC
  GGCCACCTCAGCTGCCTCGCCCGGTCCCGAGCGCAGGCCGCCACCCGCCCAGGCACCCGCGCCGCAGCCG
  GCGCCGCAGCCCGCGCCGACGCCGTCGGTGGGCAGCAGCTTCTTCAGCTCGCTGTCGCAAGCGGTGAAGC
  AGACGGCCGCCTCCGCCGGCCTGGTGGACGCGCCCGCACCTTCGGCCGCTTCCAGGAAGGCCAAGGTGCT
  GCTGGTGGTCGACGAGCCGCACACCGACTGGGCCAAATGCTTTCGGGGCAAAAAAATCCTTGGAGATTAT
  GACATCAAGGTGGAACAGGCAGAATTCTCAGAGCTTAACCTGGTGGCCCATGCAGATGGAACCTACGCCG
  TGGACATGCAGGTACTTCGGAATGGCACAAAGGTTGTCCGGTCCTTCAGACCAGACTTTGTGCTCATCCG
  ACAGCATGCATTTGGCATGGCAGAGAATGAAGACTTCCGCCACCTGGTCATTGGCATGCAGTACGCAGGC
  CTCCCCAGCATCAACTCTCTGGAGTCTATTTACAACTTCTGTGACAAGCCGTGGGTGTTTGCTCAGATGG
  TGGCCATCTTCAAGACACTGGGAGGGGAGAAATTCCCACTCATTGAGCAGACATACTACCCCAATCACCG
  AGAGATGCTTACGCTTCCTACGTTCCCTGTGGTGGTGAAGATTGGCCATGCTCACTCAGGCATGGGCAAG
  GTCAAAGTGGAAAATCACTACGACTTCCAGGACATTGCCAGTGTGGTGGCCCTCACCCAAACTTATGCCA
  CGGCAGAACCCTTCATCGACGCCAAGTATGACATCCGAGTTCAGAAGATTGGCAACAACTACAAGGCCTA
  CATGAGGACATCCATCTCAGGGAACTGGAAGACAAACACTGGCTCGGCGATGCTGGAGCAAATTGCCATG
  TCGGACCGGTACAAGCTCTGGGTAGATGCCTGCTCTGAAATGTTTGGTGGCCTGGACATCTGTGCAGTCA
  AAGCCGTGCATGGCAAAGATGGCAAAGACTACATTTTTGAGGTCATGGACTGCAGCATGCCATTGATTGG
  GGAACACCAAGTGGAGGACAGACAACTCATCACTGATCTAGTCATCAGCAAGATGAACCAGCTATTGTCC
  AGGACTCCTGCCCTGTCTCCACAGAGACCTTTAACCACCCAACAGCCACAGAGTGGAACACTTAAGGAAC
  CGGACTCCAGCAAGACCCCTCCTCAGCGGCCACCTCCCCAAGGTTGTTTACAGTATATTCTCGACTGTAA
  TGGCATTGCAGTAGGTCCAAAACAAGTCCAAGCTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225315 representing NM_013681
  Red=Cloning site Green=Tags(s)
  
  MMNFLRRRLSDSSFIANLPNGYMTDLQRPEPQQPPPAPGPGAATASAATSAASPGPERRPPPAQAPAPQP
  APQPAPTPSVGSSFFSSLSQAVKQTAASAGLVDAPAPSAASRKAKVLLVVDEPHTDWAKCFRGKKILGDY
  DIKVEQAEFSELNLVAHADGTYAVDMQVLRNGTKVVRSFRPDFVLIRQHAFGMAENEDFRHLVIGMQYAG
  LPSINSLESIYNFCDKPWVFAQMVAIFKTLGGEKFPLIEQTYYPNHREMLTLPTFPVVVKIGHAHSGMGK
  VKVENHYDFQDIASVVALTQTYATAEPFIDAKYDIRVQKIGNNYKAYMRTSISGNWKTNTGSAMLEQIAM
  SDRYKLWVDACSEMFGGLDICAVKAVHGKDGKDYIFEVMDCSMPLIGEHQVEDRQLITDLVISKMNQLLS
  RTPALSPQRPLTTQQPQSGTLKEPDSSKTPPQRPPPQGCLQYILDCNGIAVGPKQVQAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013681
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013681.3, NP_038709.1
• RefSeq Size: 3870 bp
• RefSeq ORF: 1440 bp
• Locus ID: 20965
• UniProt ID: Q64332
• Cytogenetics: 6 53.2 cM
• MW: 52.9 kDa
• Gene Summary: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.[UniProtKB/Swiss-Prot Function]