Trpc6 (NM_013838) Mouse Tagged ORF Clone

CAT#: MR225311

Trpc6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6)

Product Images

Circular map for MR225311

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trpc6
• Synonyms: AV025995; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; Trrp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225311 representing NM_013838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGAGCCCGAGGTTCGTGACCCGGAGGGGCGGCTCTCTAAAGGCTGCCCCTGGAGCCGGCACCC
  GGCGCAACGAGAGCCAGGACTATTTGCTGATGGACGAGCTGGGAGACGACGGCTACCCGCAGCTCCCGCT
  GCCACCGTATGGCTACTACCCCAGCTTCCGGGGTAATGAAAACAGACTGACTCACCGGCGGCAGACGATT
  CTTCGTGAGAAGGGAAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAATGATCATTCAACAAGCC
  TGTCTATTGAGGAAGAACGCTTTCTAGATGCAGCTGAATATGGCAACATCCCAGTGGTGCGGAAGATGCT
  AGAAGAGTGTCATTCCCTCAATGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGCTGGCTGTG
  GCCAATGAGCACTTGGAAATCACAGAGCTGCTACTCAAGAAGGAAAACTTGTCTCGAGTTGGGGATGCTT
  TACTTTTAGCCATTAGTAAAGGTTATGTACGGATTGTGGAGGCAATCCTCAACCATCCAGCTTTTGCTGA
  AGGCAAAAGGTTAGCGACAAGCCCCAGCCAGTCTGAACTTCAGCAAGATGACTTTTATGCCTATGATGAA
  GATGGGACGCGGTTCTCCCATGATGTGACCCCAATCATTCTCGCTGCACATTGCCAGGAATATGAAATTG
  TGCATACCCTCCTGAGAAAGGGTGCCCGGATTGAGCGGCCTCATGATTACTTCTGCAAGTGTACAGAATG
  CAGCCAGAAGCAGAAGCATGATTCCTTCAGCCACTCTAGATCCAGGATCAATGCATACAAAGGTCTGGCA
  AGTCCAGCATACCTGTCATTGTCCAGTGAAGATCCAGTCATGACTGCTTTAGAACTTAGCAATGAGCTGG
  CAGTGCTTGCCAACATTGAGAAAGAGTTCAAGAATGACTACAGGAAGCTGTCTATGCAGTGCAAGGATTT
  CGTTGTTGGTCTCTTGGACCTCTGCAGAAACACAGAGGAAGTGGAGGCCATCCTGAATGGGGATGCAGAG
  ACTCGCCAGCCCGGGGACTTCGGCCGTCCAAATCTCAGCCGTTTAAAACTTGCTATTAAGTATGAAGTAA
  AAAAATTTGTGGCTCATCCAAACTGTCAGCAACAGCTCCTGTCCATATGGTATGAGAACCTCTCTGGTTT
  ACGGCAGCAGACCATGGCAGTGAAGTTCCTCGTGGTCCTTGCTGTTGCCATTGGATTGCCCTTCCTGGCT
  CTCATATACTGGTGTGCTCCTTGCAGCAAGATGGGGAAGATATTGCGAGGACCGTTCATGAAGTTTGTAG
  CACACGCAGCCTCCTTCACCATTTTCCTGGGGCTGCTCGTCATGAATGCAGCTGACAGATTTGAAGGCAC
  CAAGCTCCTCCCTAATGAAACCAGCACAGATAATGCAAGGCAGCTGTTCAGGATGAAAACATCCTGTTTC
  TCATGGATGGAGATGCTCATTATATCCTGGGTAATAGGCATGATATGGGCTGAATGTAAAGAAATCTGGA
  CTCAAGGCCCCAAAGAATACTTATTTGAGTTGTGGAATATGCTTGACTTTGGAATGCTGGCAATCTTTGC
  AGCATCATTCATTGCAAGATTTATGGCGTTCTGGCATGCATCCAAAGCTCAGAGCATCATTGATGCAAAT
  GATACTTTAAAGGATTTGACAAAAGTCACACTGGGGGACAACGTTAAATACTACAATCTGGCCAGGATAA
  AGTGGGACCCTACTGATCCTCAGATCATCTCTGAAGGTCTTTATGCAATCGCTGTGGTTTTAAGTTTCTC
  CAGAATAGCTTACATTTTACCAGCAAATGAAAGCTTTGGACCTCTGCAGATTTCACTTGGAAGAACAGTG
  AAAGATATCTTCAAATTCATGGTCATATTCATCATGGTGTTTGTAGCCTTTATGATTGGAATGTTCAACC
  TTTACTCCTACTACATTGGCGCAAAACAGAATGAAGCATTCACAACAGTTGAGGAAAGTTTTAAGACACT
  GTTCTGGGCTATCTTTGGTCTTTCTGAAGTGAAGTCAGTGGTCATTAACTACAATCACAAGTTCATTGAA
  AACATCGGCTACGTTCTGTATGGTGTCTATAATGTCACAATGGTCATTGTTTTGCTAAATATGTTAATTG
  CGATGATCAATAGTTCATTCCAGGAAATTGAGGATGATGCGGACGTGGAGTGGAAGTTTGCAAGGGCCAA
  ATTGTGGTTTTCCTACTTTGAGGAGGGGAGAACACTTCCTGTCCCCTTCAATCTTGTACCAAGTCCAAAA
  TCCTTGCTTTATCTCCTATTGAAATTTAAGAAATGGATGTGTGAGCTCATCCAGGGTCAAAAGCAAGGCT
  TCCAAGAAGATGCAGAGATGAACAAGAGAAATGAAGAAAAGAAATTTGGAATTTCAGGAAGTCACGAAGA
  CCTTTCAAAATTTTCACTTGACAAAAATCAGTTGGCACACAACAAACAATCAAGTACAAGGAGCTCAGAA
  GATTATCATTTAAATAGTTTCAGTAACCCTCCAAGACAATATCAGAAAATCATGAAGAGACTCATTAAAA
  GATATGTATTGCAGGCCCAGATTGATAAGGAGAGCGATGAGGTGAATGAAGGGGAATTGAAGGAAATTAA
  GCAAGACATCTCAAGTCTCCGTTATGAACTCCTTGAAGAGAAATCACAGAACACAGAAGACCTAGCAGAG
  CTCATTAGAAAACTCGGGGAGAGACTGTCGTTAGAGCCAAAGCTGGAGGAAAGCCGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225311 representing NM_013838
  Red=Cloning site Green=Tags(s)
  
  MSQSPRFVTRRGGSLKAAPGAGTRRNESQDYLLMDELGDDGYPQLPLPPYGYYPSFRGNENRLTHRRQTI
  LREKGRRLANRGPAYMFNDHSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAV
  ANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILNHPAFAEGKRLATSPSQSELQQDDFYAYDE
  DGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCTECSQKQKHDSFSHSRSRINAYKGLA
  SPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYRKLSMQCKDFVVGLLDLCRNTEEVEAILNGDAE
  TRQPGDFGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAVKFLVVLAVAIGLPFLA
  LIYWCAPCSKMGKILRGPFMKFVAHAASFTIFLGLLVMNAADRFEGTKLLPNETSTDNARQLFRMKTSCF
  SWMEMLIISWVIGMIWAECKEIWTQGPKEYLFELWNMLDFGMLAIFAASFIARFMAFWHASKAQSIIDAN
  DTLKDLTKVTLGDNVKYYNLARIKWDPTDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTV
  KDIFKFMVIFIMVFVAFMIGMFNLYSYYIGAKQNEAFTTVEESFKTLFWAIFGLSEVKSVVINYNHKFIE
  NIGYVLYGVYNVTMVIVLLNMLIAMINSSFQEIEDDADVEWKFARAKLWFSYFEEGRTLPVPFNLVPSPK
  SLLYLLLKFKKWMCELIQGQKQGFQEDAEMNKRNEEKKFGISGSHEDLSKFSLDKNQLAHNKQSSTRSSE
  DYHLNSFSNPPRQYQKIMKRLIKRYVLQAQIDKESDEVNEGELKEIKQDISSLRYELLEEKSQNTEDLAE
  LIRKLGERLSLEPKLEESRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013838
• ORF Size: 2790 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013838.2, NP_038866.2
• RefSeq Size: 3259 bp
• RefSeq ORF: 2793 bp
• Locus ID: 22068
• UniProt ID: Q61143
• Cytogenetics: 9 2.46 cM
• MW: 107.1 kDa
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.[UniProtKB/Swiss-Prot Function]